Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DUN60_RS12060 Genome accession   NZ_CP031055
Coordinates   2747975..2749084 (-) Length   369 a.a.
NCBI ID   WP_114634024.1    Uniprot ID   -
Organism   Vibrio splendidus strain Vibrio sp     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2742975..2754084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DUN60_RS12030 - 2743305..2743805 (+) 501 WP_114634021.1 SprT family zinc-dependent metalloprotease -
  DUN60_RS12035 - 2743903..2744634 (+) 732 WP_114634022.1 endonuclease -
  DUN60_RS12040 rsmE 2744794..2745525 (+) 732 WP_065205683.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DUN60_RS12045 gshB 2745535..2746485 (+) 951 WP_004735482.1 glutathione synthase -
  DUN60_RS12050 - 2746602..2747165 (+) 564 WP_114634023.1 YqgE/AlgH family protein -
  DUN60_RS12055 ruvX 2747221..2747643 (+) 423 WP_017075420.1 Holliday junction resolvase RuvX -
  DUN60_RS12060 pilU 2747975..2749084 (-) 1110 WP_114634024.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DUN60_RS12065 pilT 2749099..2750136 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  DUN60_RS12070 - 2750163..2750867 (+) 705 WP_114634025.1 YggS family pyridoxal phosphate-dependent enzyme -
  DUN60_RS12075 proC 2750952..2751770 (+) 819 WP_114634026.1 pyrroline-5-carboxylate reductase -
  DUN60_RS12080 - 2751846..2752403 (+) 558 WP_004735475.1 YggT family protein -
  DUN60_RS12085 yggU 2752433..2752723 (+) 291 WP_050621014.1 DUF167 family protein YggU -
  DUN60_RS12090 - 2752752..2753183 (+) 432 WP_004735472.1 DUF4426 domain-containing protein -
  DUN60_RS12095 - 2753232..2753831 (+) 600 WP_114634027.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41285.20 Da        Isoelectric Point: 6.0645

>NTDB_id=302971 DUN60_RS12060 WP_114634024.1 2747975..2749084(-) (pilU) [Vibrio splendidus strain Vibrio sp]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGGKLNSAQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRKGELHELKSTMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHCADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=302971 DUN60_RS12060 WP_114634024.1 2747975..2749084(-) (pilU) [Vibrio splendidus strain Vibrio sp]
ATGGAATTGAATCAAATCCTTGAGGGGATGCTTTCTCAAAAGGCTTCGGATCTTTATATCACTGTCGATGCTCCGATCTT
GTTTCGTGTGGATGGTGAACTTCGCCCTCAAGGAGGTAAGCTGAATTCGGCTCAGGTCGCACAGTTGCTTGACGCGATGA
TGGATCAAGACCGACGTGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCCATAGTGCGTGATTTTGGTCGTTTTCGT
GTGAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTCATTCGACGTATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTACTGCAAGACCTGTCAATTGCAAAGCGTGGACTTGTGCTGGTGGTTGGGGCGACGGGCTCCGGTA
AATCAACCTCGATGGCTGCGATGACAGGTTATCGCAATACTAATCGTTCCGGGCATATTTTGACGGTTGAAGACCCAATT
GAATTCGTTCACGAACATAAAAAGTGTATCGTGACTCAACGTGAGGTTGGGCTAGACACTGAGAGTTACGAAGTTGCACT
TAAGAACTCGTTACGTCAAGCGCCAGATATGATCTTGATTGGCGAAATCCGGAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCATTTGTGTATGGCAACCTTGCACGCCAATAACGCTAACCAAGCGTTAGAGCGTATTCTCCAC
TTGGTGCCTAAAGAGCAGAAAGAGCAGTTCTTGTTTGATCTGTCGATGAATCTACGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGCAGCGGTCGTCATGGTGTGTTTGAGATTCTACTCAACAGCCCACGAGTATCTGATTTGATTCGTA
AAGGTGAGCTACATGAACTCAAGTCGACAATGGCTAAATCAAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GACTTAGTCGTAGCAGGCAAGATTAGTGAAGAGGATGCGTTCCACTGTGCTGATTCTGCTAATGACTTACGCTTAATGCT
AAAAACCAGACGCGGTGATGATGACTACGGAACTGGTGCTTTGGCTGGCGTGAAGATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.696

99.729

0.835
  pilU Pseudomonas stutzeri DSM 10701

56.873

100

0.572
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain Lp02

42.478

91.87

0.39
  pilT Legionella pneumophila strain ERS1305867

42.478

91.87

0.39
  pilT Acinetobacter baumannii strain A118

42.857

91.057

0.39
  pilT Acinetobacter baumannii D1279779

42.857

91.057

0.39
  pilT Acinetobacter nosocomialis M2

42.857

91.057

0.39
  pilT Acinetobacter baylyi ADP1

42.105

92.683

0.39
  pilT Pseudomonas aeruginosa PAK

42.09

90.786

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

87.805

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.515

0.369
  pilT Vibrio cholerae strain A1552

40.719

90.515

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.597

90.786

0.369
  pilT Neisseria gonorrhoeae MS11

39.645

91.599

0.363
  pilT Neisseria meningitidis 8013

39.645

91.599

0.363

Multiple sequence alignment