Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DS731_RS15715 Genome accession   NZ_CP031010
Coordinates   3540531..3541568 (-) Length   345 a.a.
NCBI ID   WP_119503450.1    Uniprot ID   -
Organism   Alteromonas sp. RKMC-009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3535531..3546568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DS731_RS15685 (DS731_15795) - 3535586..3537016 (+) 1431 WP_119502221.1 GAF domain-containing protein -
  DS731_RS15690 (DS731_15800) gshB 3537119..3538075 (+) 957 WP_119502222.1 glutathione synthase -
  DS731_RS15695 (DS731_15805) - 3538082..3538648 (+) 567 WP_119502223.1 YqgE/AlgH family protein -
  DS731_RS15700 (DS731_15810) ruvX 3538650..3539066 (+) 417 WP_119502224.1 Holliday junction resolvase RuvX -
  DS731_RS15705 (DS731_15815) - 3539076..3539396 (-) 321 WP_119502225.1 DUF2007 domain-containing protein -
  DS731_RS15710 (DS731_15820) pilU 3539405..3540520 (-) 1116 WP_119502226.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DS731_RS15715 (DS731_15825) pilT 3540531..3541568 (-) 1038 WP_119503450.1 type IV pilus twitching motility protein PilT Machinery gene
  DS731_RS15720 (DS731_15830) - 3541597..3542283 (+) 687 WP_119502227.1 YggS family pyridoxal phosphate-dependent enzyme -
  DS731_RS15725 (DS731_15835) proC 3542334..3543155 (+) 822 WP_119502228.1 pyrroline-5-carboxylate reductase -
  DS731_RS15730 (DS731_15840) - 3543183..3543728 (+) 546 WP_119502229.1 YggT family protein -
  DS731_RS15735 (DS731_15845) - 3543844..3544248 (+) 405 WP_119503451.1 DUF4426 domain-containing protein -
  DS731_RS15740 (DS731_15850) - 3544277..3544912 (-) 636 WP_119502230.1 glutathione S-transferase family protein -
  DS731_RS15745 (DS731_15855) - 3545059..3546057 (+) 999 WP_119502231.1 NADP-dependent oxidoreductase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38335.15 Da        Isoelectric Point: 6.6978

>NTDB_id=302806 DS731_RS15715 WP_119503450.1 3540531..3541568(-) (pilT) [Alteromonas sp. RKMC-009]
MDITELLAFSVKNKASDLHLSAGLPPIIRVDGEMRRLNIPELDHKQVHSLIYEIMNDKQRKEYEENLEVDFSFEVKDLSR
FRVNAFVQNRGAAAVLRTIPSKVLSLDDIAAPPIFRDLIDQPTGLVLVTGATGSGKSTTLAAMIDHINNTKRDHILTIED
PIEFVHENKMSVLNQREVHRDTHSFSNALRSALREDPDVVLVGELRDLETIRLAMTAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMIRSMLSESLRAVISQTLMKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQAL
QKLVALGVVSHQDAAAKSTDKNKMM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=302806 DS731_RS15715 WP_119503450.1 3540531..3541568(-) (pilT) [Alteromonas sp. RKMC-009]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAAAATAAAGCTTCCGATTTGCACTTGTCGGCAGGACTGCCGCCGAT
TATTCGTGTCGACGGGGAAATGCGCAGACTGAATATCCCTGAGCTTGATCACAAGCAGGTACATTCGCTCATTTATGAAA
TCATGAATGACAAGCAGCGCAAGGAATATGAAGAGAATCTGGAAGTTGACTTTTCCTTTGAAGTAAAGGATTTATCCCGT
TTCCGTGTTAACGCCTTTGTACAGAATCGTGGTGCTGCTGCGGTGCTTCGTACCATCCCCAGTAAAGTATTATCACTGGA
TGATATTGCTGCCCCGCCCATTTTCCGTGACCTGATTGACCAGCCTACCGGCCTTGTTCTGGTGACCGGTGCGACAGGTT
CGGGTAAAAGTACCACGCTGGCAGCCATGATTGATCACATCAATAACACCAAGCGGGACCACATTCTTACCATTGAAGAT
CCCATTGAATTTGTTCATGAAAACAAGATGAGTGTTCTGAACCAGCGTGAAGTGCACCGCGATACACACAGCTTTTCCAA
CGCGTTACGTTCGGCGCTTCGTGAAGATCCCGACGTGGTGCTGGTGGGTGAGTTACGGGACCTGGAAACCATCCGTCTTG
CGATGACTGCGGCGGAAACCGGTCACTTGGTATTTGGTACGTTGCACACCAACTCAGCGCCGAAAACCATCGACCGTATT
ATTGATGTTTTCCCGGCGGAAGAAAAAGCAATGATCCGCTCCATGTTGTCAGAATCATTGCGGGCAGTTATTTCGCAAAC
CCTGATGAAAAAAGTCGGCGGTGGCCGGGTAGCGGCACATGAGATCATGGTGGGCATACCGGCAATCCGCAACCTTATCC
GTGAAGACAAAGTGCCTCAGATGTATTCGGTTATCCAGACCGGTCAGGCGCATGGTATGCAAACCATGGATCAGGCACTG
CAAAAACTGGTTGCATTAGGCGTCGTGAGCCATCAGGATGCAGCGGCGAAATCCACCGATAAAAATAAAATGATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

75.221

98.261

0.739
  pilT Acinetobacter baumannii D1279779

75.221

98.261

0.739
  pilT Acinetobacter nosocomialis M2

74.926

98.261

0.736
  pilT Pseudomonas stutzeri DSM 10701

74.556

97.971

0.73
  pilT Pseudomonas aeruginosa PAK

74.26

97.971

0.728
  pilT Acinetobacter baylyi ADP1

74.702

97.391

0.728
  pilT Legionella pneumophila strain Lp02

69.883

99.13

0.693
  pilT Legionella pneumophila strain ERS1305867

69.883

99.13

0.693
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.681

0.681
  pilT Vibrio cholerae strain A1552

69.733

97.681

0.681
  pilT Neisseria meningitidis 8013

64.928

100

0.649
  pilT Neisseria gonorrhoeae MS11

64.638

100

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.092

100

0.414
  pilU Acinetobacter baylyi ADP1

41.329

100

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.391

Multiple sequence alignment