Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DS731_RS15710 Genome accession   NZ_CP031010
Coordinates   3539405..3540520 (-) Length   371 a.a.
NCBI ID   WP_119502226.1    Uniprot ID   -
Organism   Alteromonas sp. RKMC-009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3534405..3545520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DS731_RS21980 - 3534529..3534693 (+) 165 WP_161599167.1 hypothetical protein -
  DS731_RS15680 (DS731_15790) - 3534788..3535516 (+) 729 WP_119502220.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DS731_RS15685 (DS731_15795) - 3535586..3537016 (+) 1431 WP_119502221.1 GAF domain-containing protein -
  DS731_RS15690 (DS731_15800) gshB 3537119..3538075 (+) 957 WP_119502222.1 glutathione synthase -
  DS731_RS15695 (DS731_15805) - 3538082..3538648 (+) 567 WP_119502223.1 YqgE/AlgH family protein -
  DS731_RS15700 (DS731_15810) ruvX 3538650..3539066 (+) 417 WP_119502224.1 Holliday junction resolvase RuvX -
  DS731_RS15705 (DS731_15815) - 3539076..3539396 (-) 321 WP_119502225.1 DUF2007 domain-containing protein -
  DS731_RS15710 (DS731_15820) pilU 3539405..3540520 (-) 1116 WP_119502226.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DS731_RS15715 (DS731_15825) pilT 3540531..3541568 (-) 1038 WP_119503450.1 type IV pilus twitching motility protein PilT Machinery gene
  DS731_RS15720 (DS731_15830) - 3541597..3542283 (+) 687 WP_119502227.1 YggS family pyridoxal phosphate-dependent enzyme -
  DS731_RS15725 (DS731_15835) proC 3542334..3543155 (+) 822 WP_119502228.1 pyrroline-5-carboxylate reductase -
  DS731_RS15730 (DS731_15840) - 3543183..3543728 (+) 546 WP_119502229.1 YggT family protein -
  DS731_RS15735 (DS731_15845) - 3543844..3544248 (+) 405 WP_119503451.1 DUF4426 domain-containing protein -
  DS731_RS15740 (DS731_15850) - 3544277..3544912 (-) 636 WP_119502230.1 glutathione S-transferase family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41142.33 Da        Isoelectric Point: 5.6126

>NTDB_id=302805 DS731_RS15710 WP_119502226.1 3539405..3540520(-) (pilU) [Alteromonas sp. RKMC-009]
MKLQGYFEKATELGASDIFITVGFPVSAKIDGQLTPLGDEPLSQFQAKELVQSVMEERYIRELNELKESNFAVGITNVGR
FRCSAFWQRDSIGMVARRIVTEIPKVDELGLPAVLKDIIMSKRGLVLMVGATGTGKSTSLAALIDHRNHNSKGHILTIED
PIEFIHRHGSCVVTQREVGIDTMSFDDALKSSLRQAPDVILLGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERI
MHLAPKEMHNKLLFDLSLNLRAIVAQQLVPKKDGKGRVAAIEILLNSPFVADLIQTNRTGELKDAMKKGKEMGMQTFDMA
LYELFKEDRITYDQAIHHADSPNDLRLMIKLDSSEGNELGSLSGVSIDMDD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=302805 DS731_RS15710 WP_119502226.1 3539405..3540520(-) (pilU) [Alteromonas sp. RKMC-009]
GTGAAACTGCAGGGGTATTTTGAGAAAGCGACAGAACTCGGGGCTTCGGATATTTTTATTACCGTAGGTTTCCCTGTCAG
TGCCAAAATAGACGGGCAACTGACCCCGTTAGGCGATGAGCCGTTATCTCAGTTTCAGGCCAAGGAACTGGTTCAGAGTG
TGATGGAAGAACGTTACATCCGCGAACTTAATGAACTGAAAGAAAGTAACTTTGCAGTTGGTATTACAAATGTAGGACGT
TTTCGTTGCAGCGCATTCTGGCAACGGGACTCCATTGGTATGGTCGCCCGCCGGATTGTCACAGAAATTCCTAAAGTGGA
TGAACTGGGACTGCCTGCGGTGCTTAAAGACATTATCATGAGCAAGCGTGGTCTGGTGCTGATGGTGGGAGCTACCGGTA
CCGGTAAGTCTACCTCGCTGGCGGCCCTGATAGACCACCGCAATCACAATTCCAAAGGGCATATTCTTACTATTGAAGAT
CCCATCGAGTTTATTCACCGGCACGGTTCCTGTGTGGTTACCCAACGTGAAGTGGGCATTGATACCATGAGTTTTGATGA
CGCATTGAAAAGCTCTCTGCGTCAGGCGCCCGATGTGATCCTGCTGGGCGAAATCCGCTCCATGGAAACCATGGAATATG
CCATGTCATTTGCTGATACCGGCCACCTGTGTGTCGCGACTCTGCACGCCAATAACGCTAACCAGGCGATAGAGCGCATC
ATGCACCTTGCACCTAAAGAAATGCATAACAAGCTGCTGTTCGATCTGAGTTTGAACCTCAGGGCCATCGTTGCCCAGCA
ACTGGTGCCGAAAAAAGACGGCAAAGGCCGGGTAGCTGCCATTGAGATTCTGCTTAATTCCCCGTTTGTGGCTGATCTTA
TTCAGACAAACCGTACCGGTGAGTTGAAAGATGCCATGAAGAAAGGCAAAGAAATGGGCATGCAGACATTTGATATGGCG
CTGTATGAATTGTTTAAAGAAGACCGGATTACCTATGACCAGGCCATTCACCATGCCGATTCACCGAATGATTTACGTCT
GATGATCAAACTGGACAGCAGTGAAGGCAATGAACTGGGTTCTTTGTCCGGCGTGTCCATCGATATGGATGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.373

100

0.577
  pilU Vibrio cholerae strain A1552

55.135

99.73

0.55
  pilU Acinetobacter baylyi ADP1

54.783

92.992

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.245

93.531

0.423
  pilT Legionella pneumophila strain Lp02

41.745

86.523

0.361
  pilT Legionella pneumophila strain ERS1305867

41.745

86.523

0.361

Multiple sequence alignment