Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DAUD_RS04875 Genome accession   NC_010424
Coordinates   1026759..1027856 (+) Length   365 a.a.
NCBI ID   WP_012302067.1    Uniprot ID   -
Organism   Candidatus Desulforudis audaxviator MP104C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1021759..1032856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAUD_RS04855 (Daud_0965) - 1022158..1023405 (-) 1248 WP_012302063.1 STN domain-containing protein -
  DAUD_RS04860 (Daud_0966) - 1023420..1023917 (-) 498 WP_041570828.1 hypothetical protein -
  DAUD_RS04865 (Daud_0967) - 1024221..1024973 (+) 753 WP_166485120.1 prepilin peptidase -
  DAUD_RS04870 (Daud_0968) - 1025042..1026727 (+) 1686 WP_012302066.1 GspE/PulE family protein -
  DAUD_RS04875 (Daud_0969) pilT 1026759..1027856 (+) 1098 WP_012302067.1 type IV pilus twitching motility protein PilT Machinery gene
  DAUD_RS04880 (Daud_0970) - 1027908..1029161 (+) 1254 WP_012302068.1 type II secretion system F family protein -
  DAUD_RS04885 (Daud_0971) - 1029257..1029778 (+) 522 WP_012302069.1 GspH/FimT family pseudopilin -
  DAUD_RS04890 (Daud_0972) - 1029805..1030248 (+) 444 WP_012302070.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DAUD_RS11555 (Daud_0973) - 1030245..1030715 (+) 471 WP_012302071.1 PilW family protein -
  DAUD_RS04900 (Daud_0974) - 1030732..1031961 (+) 1230 WP_012302072.1 pilus assembly PilX N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40566.76 Da        Isoelectric Point: 7.2370

>NTDB_id=30166 DAUD_RS04875 WP_012302067.1 1026759..1027856(+) (pilT) [Candidatus Desulforudis audaxviator MP104C]
MQIDEILRLAFKLGVSDVHLTVDSPPAFRLHGALLPFDAPEWQGRLEIDPSYVRALTPADTDGLARQLIAPERYNDFLRV
GDLDFSYAIEGVGRFRVNAYKQKGNVALALRLINSRILSFSELGLPEILAYLSRRPRGMVLVTGPTGSGKSTTLASMIDL
INRERHDHIITLEDPIEFVHVHKKCRVNQREIGLDTGSFASALRAAMREDPDVILVGEMRDPETVGIALTAAETGHLVLA
TLHTSSAAQTIDRIIDVFPPHQQQQIRVQLANTIQGVVAQQLIPRIDKPGRVAAIEIMVATPAIRNLIREGKTYQIISQI
QTGAKYGMQSLDMSLRTLYQKNMISKQEALNRAADPESLRKMIEE

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=30166 DAUD_RS04875 WP_012302067.1 1026759..1027856(+) (pilT) [Candidatus Desulforudis audaxviator MP104C]
ATGCAGATAGACGAGATTCTAAGACTGGCCTTCAAACTGGGCGTGTCCGACGTGCACCTGACGGTGGATAGCCCGCCGGC
CTTCCGCCTGCACGGCGCCCTCCTGCCGTTTGATGCTCCGGAATGGCAGGGGCGGCTGGAAATCGACCCATCGTACGTGC
GGGCCTTGACTCCGGCCGATACCGACGGGCTGGCCCGGCAACTCATCGCTCCGGAGCGGTACAATGACTTTCTCCGGGTC
GGGGATTTGGACTTTTCGTACGCTATCGAGGGCGTGGGGCGCTTCCGGGTGAACGCGTACAAGCAGAAGGGCAACGTGGC
GTTGGCGCTCCGCCTGATCAACTCCAGGATTCTGTCATTCAGCGAACTCGGCCTGCCGGAAATTCTGGCCTACCTGTCCC
GCCGGCCCCGGGGTATGGTCCTGGTGACCGGTCCCACCGGCAGCGGCAAGTCGACCACCCTGGCGTCCATGATTGACCTG
ATCAACCGGGAGCGGCACGACCACATCATCACCCTGGAAGACCCGATCGAGTTTGTGCACGTGCACAAGAAATGCCGGGT
CAACCAGCGGGAGATCGGACTGGATACCGGATCCTTCGCCAGCGCCCTGCGCGCCGCTATGCGCGAGGACCCCGACGTCA
TCCTGGTGGGTGAAATGCGCGACCCGGAGACGGTCGGGATCGCCCTGACGGCGGCCGAGACCGGCCACCTGGTTTTGGCT
ACCCTGCACACCTCCAGCGCGGCCCAGACCATCGATCGGATCATCGATGTCTTCCCCCCGCACCAGCAGCAGCAGATCCG
GGTTCAACTGGCCAACACGATCCAGGGAGTGGTCGCGCAGCAACTCATCCCCCGGATCGACAAGCCGGGACGGGTGGCGG
CCATTGAAATCATGGTGGCCACGCCGGCAATACGAAACCTGATTCGCGAAGGCAAGACCTACCAGATCATCAGCCAGATT
CAGACCGGGGCGAAGTACGGAATGCAGTCGCTGGATATGAGCCTGCGCACGCTGTACCAGAAGAACATGATCAGCAAGCA
GGAGGCCCTGAACCGGGCGGCTGACCCGGAGAGTCTCAGGAAGATGATCGAGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.411

100

0.504
  pilT Acinetobacter nosocomialis M2

49.304

98.356

0.485
  pilT Acinetobacter baumannii D1279779

49.304

98.356

0.485
  pilT Acinetobacter baumannii strain A118

49.304

98.356

0.485
  pilT Pseudomonas aeruginosa PAK

49.025

98.356

0.482
  pilT Legionella pneumophila strain Lp02

49.02

97.808

0.479
  pilT Legionella pneumophila strain ERS1305867

49.02

97.808

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.468

98.356

0.477
  pilT Acinetobacter baylyi ADP1

48.189

98.356

0.474
  pilT Neisseria meningitidis 8013

46.797

98.356

0.46
  pilT Neisseria gonorrhoeae MS11

46.518

98.356

0.458
  pilT Vibrio cholerae strain A1552

46.591

96.438

0.449
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.591

96.438

0.449
  pilU Vibrio cholerae strain A1552

39.011

99.726

0.389
  pilU Pseudomonas stutzeri DSM 10701

38.462

99.726

0.384
  pilU Acinetobacter baylyi ADP1

38.356

100

0.384

Multiple sequence alignment