Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SHAL_RS05980 Genome accession   NC_010334
Coordinates   1425413..1426450 (+) Length   345 a.a.
NCBI ID   WP_012276284.1    Uniprot ID   B0TJV5
Organism   Shewanella halifaxensis HAW-EB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1420413..1431450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHAL_RS05945 (Shal_1166) hemW 1420455..1421591 (-) 1137 WP_012276277.1 radical SAM family heme chaperone HemW -
  SHAL_RS05950 (Shal_1167) rdgB 1421591..1422190 (-) 600 WP_012276278.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SHAL_RS05955 (Shal_1168) - 1422316..1422750 (-) 435 WP_012276279.1 DUF4426 domain-containing protein -
  SHAL_RS05960 (Shal_1169) yggU 1422834..1423121 (-) 288 WP_190273624.1 DUF167 family protein YggU -
  SHAL_RS05965 (Shal_1170) - 1423121..1423669 (-) 549 WP_012276281.1 YggT family protein -
  SHAL_RS05970 (Shal_1171) proC 1423705..1424523 (-) 819 WP_012276282.1 pyrroline-5-carboxylate reductase -
  SHAL_RS05975 (Shal_1172) - 1424676..1425377 (-) 702 WP_012276283.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHAL_RS05980 (Shal_1173) pilT 1425413..1426450 (+) 1038 WP_012276284.1 type IV pilus twitching motility protein PilT Machinery gene
  SHAL_RS05985 (Shal_1174) pilU 1426489..1427601 (+) 1113 WP_012276285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHAL_RS05990 (Shal_1175) hemH 1427710..1428723 (+) 1014 WP_012276286.1 ferrochelatase -
  SHAL_RS05995 (Shal_1176) ruvX 1428823..1429245 (-) 423 WP_012276287.1 Holliday junction resolvase RuvX -
  SHAL_RS06000 (Shal_1177) - 1429298..1429858 (-) 561 WP_041415865.1 YqgE/AlgH family protein -
  SHAL_RS06005 (Shal_1178) yciH 1429882..1430211 (-) 330 WP_012276289.1 stress response translation initiation inhibitor YciH -
  SHAL_RS06010 (Shal_1179) - 1430341..1430661 (-) 321 WP_012276290.1 hypothetical protein -
  SHAL_RS06015 (Shal_1180) - 1430826..1431386 (+) 561 WP_012276291.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38284.78 Da        Isoelectric Point: 6.7842

>NTDB_id=29944 SHAL_RS05980 WP_012276284.1 1425413..1426450(+) (pilT) [Shewanella halifaxensis HAW-EB4]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPGLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKRISSFPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQITREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=29944 SHAL_RS05980 WP_012276284.1 1425413..1426450(+) (pilT) [Shewanella halifaxensis HAW-EB4]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAATGCGTCAGATCTACACCTTTCAGCGGGTGTTTCTCCCAT
GATCCGTGTTGATGGTGAAGTGAGAAAGATTAATTTACCCGCTTTAGACCACCAAGGTGTGCATAGCTTAGTCTATGACA
TCATGAATGATAAGCAGCGTAAGGATTATGAAGAACATTTAGAAATCGATTTTTCGTTTGAAGTTCCTGGTCTTGCACGT
TTTCGTGTTAACGCTTTTAATCAGTCCCGTGGTGCTGCGGCAGTTTTCCGTACTATTCCTAGCGATATTTTGAGTTTAGA
ACAGTTAGGTGCGCCAGAGATCTTCAAGAGAATATCAAGTTTTCCCCGTGGTTTAGTATTAGTGACCGGTCCAACGGGGT
CAGGTAAGAGTACGACCTTGGCGGCGATGATCGATTATGTGAATGAAAACCGCCATGACCACATTCTAACCATTGAAGAT
CCGATAGAATTTGTCCATCAAAACAAGCAGTGTTTGATTAACCAACGTGAAGTCCATCGTCACACTCACAGCTTTAATGC
CGCACTTCGAAGCGCTCTTCGTGAAGATCCTGATGTGATCCTAGTCGGTGAGATGCGAGATCTTGAGACCATTCGGCTAG
CGATGACGGCGGCTGAAACCGGTCACTTAGTCTTTGGAACCTTGCATACCACTTCTGCGGCGAAAACTATTGACCGTGTG
GTCGATGTATTCCCTGAAGGTGAAAAGGGCATGGTACGTACCATGTTGTCAGAATCTTTACAGGCGGTTATCTCTCAGAC
CTTGATTAAGAAAGTGGGTGGTGGCCGAGTGGCAGCGCACGAAATTATGATGGGCACGCCTGCAATTCGTAACCTTATCC
GTGAAGATAAGGTCGCGCAGATGTATTCGGCTATCCAAACGGGTATGGCCCATGGCATGCAGACACTAGATCAGTGCTTG
CAAAACTTGGTTAATCGCGGCCAGATCACTCGTGAAGATGCCATGGCCAAGAGCTCGAATAAGCAAGCAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0TJV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.825

99.13

0.791
  pilT Acinetobacter baumannii D1279779

79.825

99.13

0.791
  pilT Acinetobacter baumannii strain A118

79.825

99.13

0.791
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.101

100

0.771
  pilT Acinetobacter baylyi ADP1

76.968

99.42

0.765
  pilT Legionella pneumophila strain Lp02

74.487

98.841

0.736
  pilT Legionella pneumophila strain ERS1305867

74.487

98.841

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.681

0.704
  pilT Vibrio cholerae strain A1552

72.107

97.681

0.704
  pilT Neisseria meningitidis 8013

68.314

99.71

0.681
  pilT Neisseria gonorrhoeae MS11

68.023

99.71

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

41.108

99.42

0.409
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment