Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SHAL_RS05985 Genome accession   NC_010334
Coordinates   1426489..1427601 (+) Length   370 a.a.
NCBI ID   WP_012276285.1    Uniprot ID   B0TJV6
Organism   Shewanella halifaxensis HAW-EB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1421489..1432601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHAL_RS05950 (Shal_1167) rdgB 1421591..1422190 (-) 600 WP_012276278.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SHAL_RS05955 (Shal_1168) - 1422316..1422750 (-) 435 WP_012276279.1 DUF4426 domain-containing protein -
  SHAL_RS05960 (Shal_1169) yggU 1422834..1423121 (-) 288 WP_190273624.1 DUF167 family protein YggU -
  SHAL_RS05965 (Shal_1170) - 1423121..1423669 (-) 549 WP_012276281.1 YggT family protein -
  SHAL_RS05970 (Shal_1171) proC 1423705..1424523 (-) 819 WP_012276282.1 pyrroline-5-carboxylate reductase -
  SHAL_RS05975 (Shal_1172) - 1424676..1425377 (-) 702 WP_012276283.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHAL_RS05980 (Shal_1173) pilT 1425413..1426450 (+) 1038 WP_012276284.1 type IV pilus twitching motility protein PilT Machinery gene
  SHAL_RS05985 (Shal_1174) pilU 1426489..1427601 (+) 1113 WP_012276285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHAL_RS05990 (Shal_1175) hemH 1427710..1428723 (+) 1014 WP_012276286.1 ferrochelatase -
  SHAL_RS05995 (Shal_1176) ruvX 1428823..1429245 (-) 423 WP_012276287.1 Holliday junction resolvase RuvX -
  SHAL_RS06000 (Shal_1177) - 1429298..1429858 (-) 561 WP_041415865.1 YqgE/AlgH family protein -
  SHAL_RS06005 (Shal_1178) yciH 1429882..1430211 (-) 330 WP_012276289.1 stress response translation initiation inhibitor YciH -
  SHAL_RS06010 (Shal_1179) - 1430341..1430661 (-) 321 WP_012276290.1 hypothetical protein -
  SHAL_RS06015 (Shal_1180) - 1430826..1431386 (+) 561 WP_012276291.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41054.89 Da        Isoelectric Point: 5.5814

>NTDB_id=29945 SHAL_RS05985 WP_012276285.1 1426489..1427601(+) (pilU) [Shewanella halifaxensis HAW-EB4]
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENPFTPAQSLDFVESLMTEAQKQEFHGTRECNFAFSAKDLGR
FRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVILVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHEHKQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHLLKDTMAKSNEQGMQTFDQA
LLKLYSDDEISYADALHYADSPNDLRLMIKLQSSDPSSNSSMDGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=29945 SHAL_RS05985 WP_012276285.1 1426489..1427601(+) (pilU) [Shewanella halifaxensis HAW-EB4]
ATGGATGTTCGTCCTTTTCTAAAGGTGATGATTGAGCGTAAGGCATCGGATCTGTTTATTACAGCAGGTTTTCCGCCAAG
CGCAAAAGTTGATGGTGAGCTGCGCCCACTCGGTGAGAATCCATTTACTCCTGCGCAGTCGTTAGACTTTGTTGAGTCGT
TAATGACTGAGGCACAGAAGCAAGAGTTTCATGGAACTCGCGAGTGTAACTTTGCCTTTTCCGCAAAAGATCTTGGTCGC
TTCCGTGTCAGTGCCTTTTGGCAGCGTGAGTTTCCCGGCTGCGTAATGCGTCGTATTGAGACACAGATCCCTGAGGTTGA
TGACTTAATGTTGCCGCCTATCTTAAAAGACTTAGTGATGAGTAAGCGCGGCCTAGTGATCTTGGTTGGTGGTACGGGAA
CGGGTAAATCGACTTCGCTTGCTGCACTGGTCGGTTATCGAAATTCCCACGCCCGTGGTCATATTCTTACCATCGAAGAT
CCGGTTGAATTTGTTCATGAACACAAGCAGAGCATTATTACTCAGCGTGAGGTGGGGATTGATACTGAATCTTTTGATGC
TGCGCTCAAAAGCTCACTGCGTCAGGCGCCGGATGTGATTTTGATTGGTGAAATTCGTAGTCAAGAAACCATGGAGTTTG
CACTGTCGTTTGCTGAAACCGGCCACCTTTGTATGGCGACCTTGCACGCTAACAACGCTAACCAAGCACTGGATCGCATC
ATGCATTTAGTACCTGAGAGCAAGCATCATCAACTGCTGTTTGATTTGTCGTTAAACCTGCGTGGTATTGTGGCGCAGCA
GCTGGTGCCCAAGAGCGATGGTAGCGGACGGCGTGCGGCGATAGAGGTGTTAATTAACACGCCGCGTGTGGCAAGCTTGA
TCGCCAAGAATGAGCTTCACTTGCTTAAAGACACCATGGCAAAGTCAAACGAGCAAGGGATGCAGACCTTCGACCAAGCG
TTATTAAAGCTCTATAGCGATGATGAGATTAGTTACGCCGATGCCTTGCATTATGCTGACTCACCAAACGATCTGCGCTT
GATGATTAAACTGCAAAGCTCCGATCCAAGCAGTAATAGCTCGATGGATGGAGTCACACTCGATTTAGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0TJV6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Vibrio cholerae strain A1552

59.73

100

0.597
  pilU Acinetobacter baylyi ADP1

58.84

97.838

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.739

93.243

0.389
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.633

86.216

0.368
  pilT Vibrio cholerae strain A1552

42.633

86.216

0.368

Multiple sequence alignment