Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DM558_RS07325 Genome accession   NZ_CP029822
Coordinates   1551101..1552120 (-) Length   339 a.a.
NCBI ID   WP_127163105.1    Uniprot ID   A0A3S9XDU7
Organism   Entomomonas moraniae strain QZS01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1546101..1557120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM558_RS07295 (DM558_07375) - 1546113..1546424 (-) 312 WP_127163093.1 SCP2 sterol-binding domain-containing protein -
  DM558_RS07300 (DM558_07380) sohB 1546591..1547619 (+) 1029 WP_127163095.1 protease SohB -
  DM558_RS07305 (DM558_07385) - 1547654..1548541 (-) 888 WP_127163097.1 alpha/beta fold hydrolase -
  DM558_RS07310 (DM558_07390) - 1548643..1549233 (+) 591 WP_127163099.1 YigZ family protein -
  DM558_RS07315 (DM558_07395) - 1549330..1549848 (+) 519 WP_127163101.1 hypothetical protein -
  DM558_RS07320 (DM558_07400) pilU 1549900..1551042 (-) 1143 WP_127163103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DM558_RS07325 (DM558_07405) pilT 1551101..1552120 (-) 1020 WP_127163105.1 type IV pilus twitching motility protein PilT Machinery gene
  DM558_RS07330 (DM558_07410) - 1552215..1552910 (+) 696 WP_127163107.1 YggS family pyridoxal phosphate-dependent enzyme -
  DM558_RS07335 (DM558_07415) - 1552996..1553571 (+) 576 WP_127163109.1 YggT family protein -
  DM558_RS07340 (DM558_07420) - 1553604..1554746 (+) 1143 WP_127163111.1 homoserine O-acetyltransferase -
  DM558_RS07345 (DM558_07425) metW 1554757..1555353 (+) 597 WP_127163113.1 methionine biosynthesis protein MetW -
  DM558_RS07350 (DM558_07430) rdgB 1555372..1555965 (+) 594 WP_127163115.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  DM558_RS07355 (DM558_07435) hemW 1555965..1557113 (+) 1149 WP_127163117.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 37609.34 Da        Isoelectric Point: 6.8896

>NTDB_id=296205 DM558_RS07325 WP_127163105.1 1551101..1552120(-) (pilT) [Entomomonas moraniae strain QZS01]
MEITELLAFSAEHKASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDRQRKDYEEKFETDFSVEVPGLAR
FRVNAFNQNRGAAAVFRTIPSKVLTMTELGMGDVFEKICNNDRGLVLVTGPTGSGKSTTLAAMVDYVNATRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKSMIRSMLSESLQAVISQRLVKKIGGGRAAAHEIMIGTSAIRNLIREDKVAQMASAIQTGAQVGMKTMEGSL
RLLVEKGLIERVHLRKFES

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=296205 DM558_RS07325 WP_127163105.1 1551101..1552120(-) (pilT) [Entomomonas moraniae strain QZS01]
ATGGAAATTACAGAGTTATTAGCCTTTAGTGCAGAGCATAAGGCGTCGGACTTACACCTCTCTGCTGGTTTACCACCAAT
GATTCGTGTAGATGGTGATGTGCGCCGTATTAATTTACCACCGTTGGATCACAAACAAGTCCACGCGCTCATTTATGACA
TTATGAATGACCGTCAACGTAAGGACTATGAAGAAAAGTTTGAAACAGACTTTTCTGTAGAGGTTCCAGGTCTTGCACGT
TTTCGGGTAAATGCTTTTAATCAAAACCGTGGTGCCGCGGCTGTATTTCGTACCATTCCCTCAAAAGTTTTGACCATGAC
TGAACTTGGTATGGGTGATGTATTTGAGAAAATCTGTAATAATGACCGTGGACTTGTATTAGTTACAGGGCCTACAGGGT
CCGGTAAGTCAACAACGTTAGCTGCAATGGTCGATTATGTGAATGCAACACGTTACGAACATATTTTAACCATTGAAGAC
CCTATTGAGTTCGTGCATGAATCAAAAAAATGCCTTGTCAATCAGCGTGAAGTACACCGTGATACATTGGGCTTTAATGA
AGCGCTTCGTAGTGCATTGCGTGAAGACCCCGATATTATTCTAGTGGGTGAGTTGCGTGACTTAGAAACCATTCGATTGG
CATTAACAGCGGCAGAAACAGGACACTTGGTCTTCGGTACTTTGCATACCAGTTCTGCGGCTAAAACGATTGACCGTATT
ATTGACGTATTCCCAGCTGCTGAAAAATCAATGATTCGTTCGATGTTATCAGAGTCTTTGCAAGCCGTAATTTCTCAGCG
TTTAGTTAAAAAGATTGGTGGCGGGCGAGCGGCGGCTCATGAAATTATGATTGGAACCTCGGCCATTCGTAACCTTATTC
GTGAAGATAAAGTGGCTCAAATGGCTTCTGCAATTCAAACGGGCGCGCAAGTCGGTATGAAGACAATGGAAGGTAGTTTA
AGGCTACTGGTAGAAAAAGGATTGATTGAACGCGTTCATCTAAGAAAATTTGAATCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9XDU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

83.333

99.115

0.826
  pilT Pseudomonas stutzeri DSM 10701

83.686

97.64

0.817
  pilT Acinetobacter baylyi ADP1

80.547

97.05

0.782
  pilT Acinetobacter baumannii D1279779

80.243

97.05

0.779
  pilT Acinetobacter nosocomialis M2

80.243

97.05

0.779
  pilT Acinetobacter baumannii strain A118

80.243

97.05

0.779
  pilT Legionella pneumophila strain Lp02

74.924

97.64

0.732
  pilT Legionella pneumophila strain ERS1305867

74.924

97.64

0.732
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.394

97.345

0.676
  pilT Vibrio cholerae strain A1552

69.394

97.345

0.676
  pilT Neisseria meningitidis 8013

66.667

97.345

0.649
  pilT Neisseria gonorrhoeae MS11

66.364

97.345

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.121

97.345

0.507
  pilU Vibrio cholerae strain A1552

41.538

95.87

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.303

97.345

0.392
  pilU Acinetobacter baylyi ADP1

38.509

94.985

0.366

Multiple sequence alignment