Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DM558_RS07320 Genome accession   NZ_CP029822
Coordinates   1549900..1551042 (-) Length   380 a.a.
NCBI ID   WP_127163103.1    Uniprot ID   A0A3S9XDT9
Organism   Entomomonas moraniae strain QZS01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1544900..1556042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM558_RS07290 (DM558_07370) - 1545320..1546096 (-) 777 WP_127163091.1 TSUP family transporter -
  DM558_RS07295 (DM558_07375) - 1546113..1546424 (-) 312 WP_127163093.1 SCP2 sterol-binding domain-containing protein -
  DM558_RS07300 (DM558_07380) sohB 1546591..1547619 (+) 1029 WP_127163095.1 protease SohB -
  DM558_RS07305 (DM558_07385) - 1547654..1548541 (-) 888 WP_127163097.1 alpha/beta fold hydrolase -
  DM558_RS07310 (DM558_07390) - 1548643..1549233 (+) 591 WP_127163099.1 YigZ family protein -
  DM558_RS07315 (DM558_07395) - 1549330..1549848 (+) 519 WP_127163101.1 hypothetical protein -
  DM558_RS07320 (DM558_07400) pilU 1549900..1551042 (-) 1143 WP_127163103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DM558_RS07325 (DM558_07405) pilT 1551101..1552120 (-) 1020 WP_127163105.1 type IV pilus twitching motility protein PilT Machinery gene
  DM558_RS07330 (DM558_07410) - 1552215..1552910 (+) 696 WP_127163107.1 YggS family pyridoxal phosphate-dependent enzyme -
  DM558_RS07335 (DM558_07415) - 1552996..1553571 (+) 576 WP_127163109.1 YggT family protein -
  DM558_RS07340 (DM558_07420) - 1553604..1554746 (+) 1143 WP_127163111.1 homoserine O-acetyltransferase -
  DM558_RS07345 (DM558_07425) metW 1554757..1555353 (+) 597 WP_127163113.1 methionine biosynthesis protein MetW -
  DM558_RS07350 (DM558_07430) rdgB 1555372..1555965 (+) 594 WP_127163115.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42418.95 Da        Isoelectric Point: 6.3128

>NTDB_id=296204 DM558_RS07320 WP_127163103.1 1549900..1551042(-) (pilU) [Entomomonas moraniae strain QZS01]
MEFEHLLQTIVDKKASDLFLTVGVPPCIKVDGKLSPLPLERLSGERVKELARKLMSERQQKEFVTTQECNFAVSYPGVGR
FRVSAYIQRNQVGMVLRRIVTDIPTIDQLGLPKMIKDLAMVKRGLVLFVGATGAGKSTSLAAMVDYRNTNSTGHIISIED
PIEYLFSHKNCIVTQREVGLDTESFEVALKNTLRQAPDVIMIGEVRTRQTMEYALAFAETGHLCLATLHANNANQALDRI
ISFFAPERHSQIWLDLSLNLRAIVAQQLIPDKSGMGRHAAVEILLGTPLVSDMIRTGQVTELKGIMGRSVELGMQTFDQA
LFSLFERGMITYDSALACADSVNDLRLMIKLHSEKQGTQKKNDFSGVKLRDYDEEDEDDI

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=296204 DM558_RS07320 WP_127163103.1 1549900..1551042(-) (pilU) [Entomomonas moraniae strain QZS01]
ATGGAGTTTGAACACCTATTACAGACTATTGTCGATAAAAAGGCCTCTGACTTATTTTTGACTGTTGGCGTGCCTCCTTG
TATTAAAGTGGATGGAAAGCTTTCTCCTTTGCCTTTGGAAAGGCTCTCGGGCGAAAGAGTAAAGGAGCTCGCACGTAAGC
TGATGAGTGAGCGCCAGCAAAAAGAGTTTGTTACAACTCAAGAGTGTAATTTCGCAGTCAGTTATCCAGGTGTTGGACGT
TTTCGTGTTAGTGCTTATATCCAGCGAAACCAAGTCGGAATGGTATTGCGTCGTATTGTGACAGATATTCCGACCATTGA
TCAGCTAGGTCTCCCTAAGATGATTAAAGATCTAGCCATGGTAAAGCGTGGTTTAGTATTATTTGTTGGGGCAACTGGGG
CAGGTAAATCTACATCATTAGCTGCCATGGTAGATTACCGTAATACCAATTCAACAGGTCATATTATTTCTATTGAAGAC
CCCATTGAGTATTTGTTTAGCCATAAAAACTGTATTGTGACCCAACGTGAAGTAGGGCTGGATACAGAATCGTTTGAAGT
TGCTCTAAAAAATACATTACGTCAAGCACCTGATGTGATTATGATTGGTGAGGTACGTACTCGTCAAACCATGGAGTATG
CCTTAGCATTTGCTGAAACGGGACATCTTTGTTTGGCGACGTTGCATGCGAATAATGCTAACCAAGCGTTGGACCGTATT
ATCAGTTTCTTTGCTCCAGAACGTCATTCACAAATATGGTTAGATTTATCTTTAAACTTACGTGCTATCGTTGCTCAACA
ATTAATTCCTGATAAGTCCGGTATGGGGCGACATGCTGCGGTGGAGATATTGTTAGGTACTCCACTTGTTTCGGATATGA
TTCGGACGGGGCAAGTTACTGAGCTAAAAGGCATTATGGGGCGTTCAGTTGAGTTAGGTATGCAGACGTTTGACCAAGCA
CTGTTTAGCTTGTTTGAGCGAGGAATGATTACTTATGATTCAGCCTTAGCATGCGCAGACTCAGTCAATGACTTACGTTT
GATGATTAAGTTACATTCCGAAAAACAGGGTACACAAAAGAAAAATGATTTCTCAGGCGTTAAGTTAAGGGACTATGATG
AAGAGGATGAAGACGATATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9XDT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.8

98.684

0.639
  pilU Acinetobacter baylyi ADP1

58.028

93.421

0.542
  pilU Vibrio cholerae strain A1552

55.256

97.632

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

89.211

0.395
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.158

0.366
  pilT Pseudomonas aeruginosa PAK

41.194

88.158

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.515

86.842

0.361
  pilT Vibrio cholerae strain A1552

41.515

86.842

0.361

Multiple sequence alignment