Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DM292_RS20675 Genome accession   NZ_CP029772
Coordinates   4411027..4412061 (+) Length   344 a.a.
NCBI ID   WP_017245436.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain R2A2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4406027..4417061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM292_RS20655 (DM292_20655) - 4406597..4408564 (-) 1968 WP_110778823.1 dynamin-like GTPase family protein -
  DM292_RS20660 (DM292_20660) - 4408836..4409426 (-) 591 WP_110778824.1 YggT family protein -
  DM292_RS20665 (DM292_20665) proC 4409436..4410260 (-) 825 WP_110778825.1 pyrroline-5-carboxylate reductase -
  DM292_RS20670 (DM292_20670) - 4410271..4410960 (-) 690 WP_110778826.1 YggS family pyridoxal phosphate-dependent enzyme -
  DM292_RS20675 (DM292_20675) pilT 4411027..4412061 (+) 1035 WP_017245436.1 type IV pilus twitching motility protein PilT Machinery gene
  DM292_RS20680 (DM292_20680) pilU 4412122..4413267 (+) 1146 WP_026006585.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DM292_RS20685 (DM292_20685) - 4413274..4414098 (-) 825 WP_110778827.1 TIGR03915 family putative DNA repair protein -
  DM292_RS20690 (DM292_20690) - 4414098..4415312 (-) 1215 WP_110778828.1 putative DNA modification/repair radical SAM protein -
  DM292_RS20695 (DM292_20695) - 4415443..4415838 (+) 396 WP_110778829.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38075.85 Da        Isoelectric Point: 6.8359

>NTDB_id=296000 DM292_RS20675 WP_017245436.1 4411027..4412061(+) (pilT) [Stutzerimonas frequens strain R2A2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEDLNMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLLAKGLISRDSAREKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=296000 DM292_RS20675 WP_017245436.1 4411027..4412061(+) (pilT) [Stutzerimonas frequens strain R2A2]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGCATCAACCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAAACCGACTTCTCGTTCGAGGTGCCGGGCGTGGCGCGT
TTCCGCGTCAACGCGTTCAATCAGAACCGCGGCTCGGGCGCGGTATTCCGGACCATTCCTTCCAAGGTGCTGACCATGGA
AGACCTGAACATGGGCGAGGTCTTCCGCAAGATCACCGACGTGCCGCGCGGGTTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGTAAGTCGACTACCCTGGCGGCGATGCTCGACTATCTAAACAACACCAAATACCACCATATCCTTACCATCGAAGAC
CCCATCGAATTCGTTCACGAATCGAAGAAATGTCTGGTCAACCAGCGCGAGGTACACCGCGATACCCTCGGCTTCTCCGA
AGCCCTACGCTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGGGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCCGCCAAGACCATCGACCGTGTG
GTCGACGTGTTCCCGGCCGAAGAGAAGTCCATGGTCCGTTCGATGCTCTCCGAATCCCTGCAGGCTGTGATCTCGCAGAC
GCTGCTGAAGAAGGTTGGCGGCGGTCGCGTGGCGGCCCACGAGATCATGATCGGTACGCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGTTCGCTGGGCATGCAGACCCTGGACATGTGCCTC
AAGGGGCTGCTGGCCAAGGGCCTGATCAGCCGCGACAGCGCGCGCGAGAAGGCCAAGCAGCCAGAGAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

97.965

100

0.98
  pilT Pseudomonas aeruginosa PAK

94.477

100

0.945
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

73.964

98.256

0.727
  pilT Legionella pneumophila strain ERS1305867

73.964

98.256

0.727
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment