Detailed information NTG0296001

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DM292_RS20680
• Genome accession: NZ_CP029772
• Coordinates: 4412122..4413267 (+)
• Length: 381 a.a.
• NCBI ID: WP_026006585.1
• Uniprot ID: A0AA47I1L4
• Organism: Stutzerimonas frequens strain R2A2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4407122..4418267

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DM292_RS20660 (DM292_20660)	-	4408836..4409426 (-)	591	WP_110778824.1	YggT family protein	-
DM292_RS20665 (DM292_20665)	proC	4409436..4410260 (-)	825	WP_110778825.1	pyrroline-5-carboxylate reductase	-
DM292_RS20670 (DM292_20670)	-	4410271..4410960 (-)	690	WP_110778826.1	YggS family pyridoxal phosphate-dependent enzyme	-
DM292_RS20675 (DM292_20675)	pilT	4411027..4412061 (+)	1035	WP_017245436.1	type IV pilus twitching motility protein PilT	Machinery gene
DM292_RS20680 (DM292_20680)	pilU	4412122..4413267 (+)	1146	WP_026006585.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DM292_RS20685 (DM292_20685)	-	4413274..4414098 (-)	825	WP_110778827.1	TIGR03915 family putative DNA repair protein	-
DM292_RS20690 (DM292_20690)	-	4414098..4415312 (-)	1215	WP_110778828.1	putative DNA modification/repair radical SAM protein	-
DM292_RS20695 (DM292_20695)	-	4415443..4415838 (+)	396	WP_110778829.1	TM2 domain-containing protein	-
DM292_RS20700 (DM292_20700)	-	4415893..4417167 (-)	1275	WP_110778830.1	dihydroorotase	-
DM292_RS20705 (DM292_20705)	-	4417167..4418183 (-)	1017	WP_017245430.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42540.84 Da
• Isoelectric Point: 6.8856

>NTDB_id=296001 DM292_RS20680 WP_026006585.1 4412122..4413267(+) (pilU) [Stutzerimonas frequens strain R2A2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTTVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=296001 DM292_RS20680 WP_026006585.1 4412122..4413267(+) (pilU) [Stutzerimonas frequens strain R2A2]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATTACGGCGGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACCGCCATGTCACCGGAGATGACCCGCGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAAAATCACGAATGCAACTTCGCCATCAGTGCCCGCGGGATCGGTCGC
TTCCGTGTCAGTGCGTTCTATCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATTCCGACCATCGA
AGAGCTGAAGCTGCCTGACGTTCTCAAGAAGCTGTCGATGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACGATGGACTATG
CCGTAGCCTTCGCCGAGACCGGACACCTGTGTCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGATCGGCACAACCAGGTCTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACACCCGACGGCAAGGGTCGTCGCGCGGTGATCGAGGTGTTGATCAACACCCCGCTGGCCGCGGACCTTA
TCCGCAAGGGCGAGGTGCATGAACTCAAGTCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCGCTGTTGCACGCCGACTCGGCCAACGACCTGCGACT
GTTGATCAAGCTGGGTTCGGAAACCGACGGCGAGCACCTGACCACTGTTTCGCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388