Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DLK06_RS15300 Genome accession   NZ_CP029610
Coordinates   3127919..3128956 (+) Length   345 a.a.
NCBI ID   WP_002121749.1    Uniprot ID   A0A009I411
Organism   Acinetobacter pittii strain ST220     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3122919..3133956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLK06_RS15290 (DLK06_15310) sbcC 3123303..3126899 (+) 3597 WP_032057668.1 SbcC/MukB-like Walker B domain-containing protein -
  DLK06_RS15295 (DLK06_15315) yggS 3127100..3127792 (-) 693 WP_032057669.1 YggS family pyridoxal phosphate-dependent enzyme -
  DLK06_RS15300 (DLK06_15320) pilT 3127919..3128956 (+) 1038 WP_002121749.1 type IV pilus twitching motility protein PilT Machinery gene
  DLK06_RS15305 (DLK06_15325) pilU 3128976..3130094 (+) 1119 WP_032057670.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DLK06_RS15310 (DLK06_15330) fur 3130164..3130601 (-) 438 WP_002121758.1 ferric iron uptake transcriptional regulator -
  DLK06_RS15315 (DLK06_15335) omlA 3130713..3131111 (+) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  DLK06_RS15320 (DLK06_15340) - 3131176..3131490 (-) 315 WP_017481335.1 RnfH family protein -
  DLK06_RS15325 (DLK06_15345) - 3131490..3132557 (-) 1068 WP_031944146.1 membrane protein -
  DLK06_RS15335 (DLK06_15355) - 3132711..3133166 (-) 456 WP_002121698.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38389.97 Da        Isoelectric Point: 6.7269

>NTDB_id=294195 DLK06_RS15300 WP_002121749.1 3127919..3128956(+) (pilT) [Acinetobacter pittii strain ST220]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=294195 DLK06_RS15300 WP_002121749.1 3127919..3128956(+) (pilT) [Acinetobacter pittii strain ST220]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACACCTATCTGCTGGCATGCCACCAAT
GATTCGCGTTGATGGTGAAGTCCGTCGCATTAACTTACCTGCTCTAGATCATAAAGATGTTCATCGTTTGGTCTACGACA
TCATGAATGATAAACAGCGTCGTGACTACGAAGAAAAACTCGAAACCGACTTTTCTTTTGAAGTGCCCAACGTTGCTCGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCGGGTGCAGTGTTTCGTACCATTCCATCCAAAGTACTCACCATGGA
AGATTTAGGCTTAGGACAAATCTTTAAAGATATTTGTGACTATCCTCGCGGGATTGTACTGGTGACAGGCCCAACAGGTT
CTGGTAAATCGACCACACTTGCAGCGATGATGGATTATATCAACGAAAACCGTTATGACCATATTTTAACGGTTGAAGAC
CCTATCGAATTTGTACACCAGTCTAAGAAATGTCTCATCAATCAACGTGAAGTGCATCGTGATACACATGGCTTTAATGA
GGCGCTACGTTCGGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAGACTATTCGTTTAG
CACTTACTGCTGCCGAAACAGGTCATTTGGTGTTTGGTACGCTTCATACCACTTCTGCTGCAAAAACGATTGACCGTGTA
ATTGACGTATTCCCAGCCGAAGAAAAAGATATGGTTCGTGCCATGCTCTCTGAATCATTGCAGGCTGTTATTTCACAAAC
CTTGCTTAAAAAGAATGGCGGCGGTCGTGTCGCCGCTCATGAAATCATGATTGGTATTCCTGCGATTCGTAACTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCTATTCAGACTGGCGCAAATCATGGCATGACCACCCTTGACCAAAGCTTA
AAGAATTTGGTGTCTCGCGGAGTGATCAGCCCGCAAACGGCTCGTACTGCTGCTAAACAGCCTGAATCATTTCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009I411

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

98.841

100

0.988
  pilT Acinetobacter baumannii strain A118

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment