Detailed information NTG0294196

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DLK06_RS15305
• Genome accession: NZ_CP029610
• Coordinates: 3128976..3130094 (+)
• Length: 372 a.a.
• NCBI ID: WP_032057670.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain ST220
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3123976..3135094

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DLK06_RS15295 (DLK06_15315)	yggS	3127100..3127792 (-)	693	WP_032057669.1	YggS family pyridoxal phosphate-dependent enzyme	-
DLK06_RS15300 (DLK06_15320)	pilT	3127919..3128956 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
DLK06_RS15305 (DLK06_15325)	pilU	3128976..3130094 (+)	1119	WP_032057670.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DLK06_RS15310 (DLK06_15330)	fur	3130164..3130601 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
DLK06_RS15315 (DLK06_15335)	omlA	3130713..3131111 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
DLK06_RS15320 (DLK06_15340)	-	3131176..3131490 (-)	315	WP_017481335.1	RnfH family protein	-
DLK06_RS15325 (DLK06_15345)	-	3131490..3132557 (-)	1068	WP_031944146.1	membrane protein	-
DLK06_RS15335 (DLK06_15355)	-	3132711..3133166 (-)	456	WP_002121698.1	bacteriohemerythrin	-
DLK06_RS15340 (DLK06_15360)	-	3133418..3134266 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41975.16 Da
• Isoelectric Point: 6.5062

>NTDB_id=294196 DLK06_RS15305 WP_032057670.1 3128976..3130094(+) (pilU) [Acinetobacter pittii strain ST220]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEDPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=294196 DLK06_RS15305 WP_032057670.1 3128976..3130094(+) (pilU) [Acinetobacter pittii strain ST220]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAATTTTGCGATTATGAACCGCGATAAAAGTGCG
CGCTTTCGTGTCAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCTTTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGTTGCATCATTACCCAACGTGAAGTCGGGATTGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCTCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTCGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGATCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363