Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SPEA_RS05900 Genome accession   NC_009901
Coordinates   1365400..1366437 (+) Length   345 a.a.
NCBI ID   WP_012154383.1    Uniprot ID   A8H1L8
Organism   Shewanella pealeana ATCC 700345     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1360400..1371437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPEA_RS05870 (Spea_1122) rdgB 1361527..1362126 (-) 600 WP_012154377.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SPEA_RS05875 (Spea_1123) - 1362253..1362687 (-) 435 WP_012154378.1 DUF4426 domain-containing protein -
  SPEA_RS05880 (Spea_1124) yggU 1362771..1363043 (-) 273 WP_012154379.1 DUF167 family protein YggU -
  SPEA_RS05885 (Spea_1125) - 1363058..1363606 (-) 549 WP_012154380.1 YggT family protein -
  SPEA_RS05890 (Spea_1126) proC 1363642..1364460 (-) 819 WP_012154381.1 pyrroline-5-carboxylate reductase -
  SPEA_RS05895 (Spea_1127) - 1364663..1365364 (-) 702 WP_012154382.1 YggS family pyridoxal phosphate-dependent enzyme -
  SPEA_RS05900 (Spea_1128) pilT 1365400..1366437 (+) 1038 WP_012154383.1 type IV pilus twitching motility protein PilT Machinery gene
  SPEA_RS05905 (Spea_1129) pilU 1366461..1367573 (+) 1113 WP_012154384.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SPEA_RS05910 (Spea_1130) hemH 1367696..1368709 (+) 1014 WP_012154385.1 ferrochelatase -
  SPEA_RS05915 (Spea_1131) ruvX 1368868..1369293 (-) 426 WP_012154386.1 Holliday junction resolvase RuvX -
  SPEA_RS05920 (Spea_1132) - 1369332..1369892 (-) 561 WP_041410859.1 YqgE/AlgH family protein -
  SPEA_RS05925 (Spea_1133) yciH 1369917..1370246 (-) 330 WP_012154388.1 stress response translation initiation inhibitor YciH -
  SPEA_RS05930 (Spea_1134) - 1370648..1370968 (-) 321 WP_041410860.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38302.79 Da        Isoelectric Point: 6.7842

>NTDB_id=29353 SPEA_RS05900 WP_012154383.1 1365400..1366437(+) (pilT) [Shewanella pealeana ATCC 700345]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPGLAR
FRVNAFNQSRGSAAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQISREDAMAKSSNKQTNF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=29353 SPEA_RS05900 WP_012154383.1 1365400..1366437(+) (pilT) [Shewanella pealeana ATCC 700345]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAATGCATCAGATCTACACCTTTCAGCGGGTGTTTCTCCCAT
GATCCGTGTTGATGGTGAAGTGAGAAAGATTAATTTACCCGCTTTAGATCACCAAGGTGTACATAGCTTAGTCTACGACA
TCATGAACGATAAGCAGCGTAAGGATTATGAAGAGCACCTAGAGATAGATTTCTCGTTTGAAGTGCCGGGTCTTGCACGT
TTTCGTGTTAATGCTTTTAACCAGTCTCGTGGTTCGGCGGCGGTTTTCCGTACCATTCCCAGTGACATCTTGAGTCTGGA
GCAATTAGGCGCACCGGAGATCTTCAAAAAAATCTCAAGTTTCCCCCGTGGTCTAGTATTGGTCACCGGCCCAACTGGCT
CGGGAAAGAGTACCACCTTGGCGGCAATGATCGATTATGTGAACGAAAACCGTCACGACCACATTCTGACCATTGAAGAT
CCGATAGAATTTGTTCATCAAAATAAGCAGTGCTTGATTAACCAACGTGAAGTGCATCGCCATACCCACAGCTTTAACGC
GGCACTGAGAAGTGCACTGCGTGAAGATCCAGACGTGATCTTAGTCGGTGAGATGCGAGATCTTGAAACCATTCGTCTGG
CGATGACAGCGGCGGAAACGGGTCACTTAGTCTTTGGAACCCTGCATACCACCTCTGCGGCTAAAACCATTGACCGTATT
GTCGACGTTTTCCCTGAAGGTGAGAAAGGCATGGTAAGAACCATGTTGTCTGAATCTTTGCAGGCGGTTATCTCTCAGAC
CTTGATTAAGAAAGTGGGCGGCGGCCGAGTGGCGGCGCATGAGATCATGATGGGGACGCCTGCGATTCGTAACCTTATTC
GTGAGGATAAAGTCGCGCAGATGTATTCGGCAATTCAAACGGGTATGGCCCACGGAATGCAGACGCTGGACCAATGTTTA
CAGAACTTGGTTAATCGCGGCCAAATCTCCCGTGAAGATGCCATGGCTAAGAGCTCGAATAAACAAACCAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8H1L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.532

99.13

0.788
  pilT Acinetobacter baumannii D1279779

79.532

99.13

0.788
  pilT Acinetobacter baumannii strain A118

79.532

99.13

0.788
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.101

100

0.771
  pilT Acinetobacter baylyi ADP1

76.968

99.42

0.765
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.356

97.681

0.394

Multiple sequence alignment