Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SPEA_RS05905 Genome accession   NC_009901
Coordinates   1366461..1367573 (+) Length   370 a.a.
NCBI ID   WP_012154384.1    Uniprot ID   A8H1L9
Organism   Shewanella pealeana ATCC 700345     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1361461..1372573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPEA_RS05870 (Spea_1122) rdgB 1361527..1362126 (-) 600 WP_012154377.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SPEA_RS05875 (Spea_1123) - 1362253..1362687 (-) 435 WP_012154378.1 DUF4426 domain-containing protein -
  SPEA_RS05880 (Spea_1124) yggU 1362771..1363043 (-) 273 WP_012154379.1 DUF167 family protein YggU -
  SPEA_RS05885 (Spea_1125) - 1363058..1363606 (-) 549 WP_012154380.1 YggT family protein -
  SPEA_RS05890 (Spea_1126) proC 1363642..1364460 (-) 819 WP_012154381.1 pyrroline-5-carboxylate reductase -
  SPEA_RS05895 (Spea_1127) - 1364663..1365364 (-) 702 WP_012154382.1 YggS family pyridoxal phosphate-dependent enzyme -
  SPEA_RS05900 (Spea_1128) pilT 1365400..1366437 (+) 1038 WP_012154383.1 type IV pilus twitching motility protein PilT Machinery gene
  SPEA_RS05905 (Spea_1129) pilU 1366461..1367573 (+) 1113 WP_012154384.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SPEA_RS05910 (Spea_1130) hemH 1367696..1368709 (+) 1014 WP_012154385.1 ferrochelatase -
  SPEA_RS05915 (Spea_1131) ruvX 1368868..1369293 (-) 426 WP_012154386.1 Holliday junction resolvase RuvX -
  SPEA_RS05920 (Spea_1132) - 1369332..1369892 (-) 561 WP_041410859.1 YqgE/AlgH family protein -
  SPEA_RS05925 (Spea_1133) yciH 1369917..1370246 (-) 330 WP_012154388.1 stress response translation initiation inhibitor YciH -
  SPEA_RS05930 (Spea_1134) - 1370648..1370968 (-) 321 WP_041410860.1 hypothetical protein -
  SPEA_RS05935 (Spea_1135) - 1371140..1371697 (+) 558 WP_012154390.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40978.84 Da        Isoelectric Point: 6.0489

>NTDB_id=29354 SPEA_RS05905 WP_012154384.1 1366461..1367573(+) (pilU) [Shewanella pealeana ATCC 700345]
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENAFTPAQSLDFVESLMTDVQKQEFHGTRECNFAFSAKDLGR
FRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVILVGGTGTGKSTSLAALVGYRNTHARGHILTIED
PVEFVHEHKQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHTLKETMAKSNEQGMQTFDQA
LLKLYSEGEISYADALHHADSPNDLRLMIKLQSSDTTSSSSMDGVTLNLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=29354 SPEA_RS05905 WP_012154384.1 1366461..1367573(+) (pilU) [Shewanella pealeana ATCC 700345]
ATGGATGTTCGTCCTTTTCTAAAGGTGATGATTGAGCGTAAGGCATCGGATCTATTTATTACCGCAGGTTTCCCACCGAG
CGCTAAAGTTGATGGTGAGCTGCGCCCCTTAGGTGAGAATGCTTTTACTCCTGCCCAGTCGTTAGATTTTGTTGAGTCAT
TGATGACTGATGTGCAAAAGCAGGAATTTCACGGGACTCGTGAGTGTAACTTTGCCTTTTCGGCTAAAGATCTTGGCCGA
TTTCGTGTCAGCGCCTTTTGGCAGCGTGAGTTTCCCGGCTGCGTGATGCGTCGAATCGAGACTCAGATCCCTGAGGTTGA
TGACTTAATGTTGCCGCCTATCTTAAAAGATTTGGTGATGAGTAAGCGCGGCTTAGTGATTTTGGTTGGTGGTACCGGTA
CAGGTAAGTCGACCTCGCTTGCGGCACTGGTCGGCTATCGAAATACCCATGCCCGCGGTCATATTCTGACGATTGAAGAT
CCGGTGGAATTTGTTCATGAGCATAAGCAGAGCATCATTACTCAGCGTGAGGTGGGGATCGATACCGAGTCTTTCGATGC
AGCGCTTAAAAGTTCTCTGCGTCAGGCGCCTGATGTGATTTTGATCGGTGAAATTCGTAGCCAGGAAACTATGGAGTTCG
CGCTGTCTTTTGCCGAGACGGGTCACCTTTGTATGGCGACTCTGCATGCTAATAACGCTAACCAAGCGCTCGACCGTATC
ATGCATTTAGTGCCGGAGAGTAAGCACCATCAACTGCTGTTTGATTTGTCGTTAAACTTACGCGGTATTGTGGCGCAGCA
ATTGGTGCCTAAGAGCGATGGCAGCGGAAGGCGTGCGGCGATTGAGGTATTGATTAATACCCCGCGTGTCGCCAGCTTGA
TTGCTAAAAATGAGCTGCATACCCTTAAAGAAACCATGGCAAAGTCAAATGAGCAGGGGATGCAGACCTTCGACCAAGCT
TTGTTAAAGCTCTACAGCGAGGGCGAGATCAGTTACGCCGATGCGCTGCATCATGCGGATTCACCAAACGACCTGCGACT
GATGATCAAGCTACAAAGCTCAGATACGACTAGCAGTAGCTCTATGGATGGGGTCACACTCAATTTAGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8H1L9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.459

100

0.595
  pilU Pseudomonas stutzeri DSM 10701

61.756

95.405

0.589
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.243

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter baylyi ADP1

39.766

92.432

0.368
  pilT Acinetobacter nosocomialis M2

39.766

92.432

0.368
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365

Multiple sequence alignment