Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DCD74_RS00825 Genome accession   NZ_CP029556
Coordinates   178341..179378 (+) Length   345 a.a.
NCBI ID   WP_112925647.1    Uniprot ID   A0A344J315
Organism   Solilutibacter oculi strain 83-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 173341..184378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCD74_RS00785 (DCD74_00785) - 173692..173895 (+) 204 WP_112927591.1 dodecin family protein -
  DCD74_RS00790 (DCD74_00790) pdxH 173929..174531 (+) 603 WP_112927592.1 pyridoxamine 5'-phosphate oxidase -
  DCD74_RS00795 (DCD74_00795) - 174531..174887 (+) 357 WP_112925642.1 hypothetical protein -
  DCD74_RS00800 (DCD74_00800) - 174884..175708 (+) 825 WP_112925643.1 cobalamin-binding protein -
  DCD74_RS00805 (DCD74_00805) - 175678..176196 (-) 519 WP_237049676.1 GNAT family N-acetyltransferase -
  DCD74_RS00810 (DCD74_00810) - 176196..176684 (-) 489 WP_162615819.1 DUF4426 domain-containing protein -
  DCD74_RS00815 (DCD74_00815) proC 176687..177529 (-) 843 WP_112925645.1 pyrroline-5-carboxylate reductase -
  DCD74_RS00820 (DCD74_00820) - 177558..178184 (-) 627 WP_237049628.1 YggS family pyridoxal phosphate-dependent enzyme -
  DCD74_RS00825 (DCD74_00825) pilT 178341..179378 (+) 1038 WP_112925647.1 type IV pilus twitching motility protein PilT Machinery gene
  DCD74_RS00830 (DCD74_00830) pilU 179421..180554 (+) 1134 WP_112925648.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DCD74_RS00835 (DCD74_00835) - 180603..181169 (+) 567 WP_112925649.1 YqgE/AlgH family protein -
  DCD74_RS00840 (DCD74_00840) ruvX 181166..181630 (+) 465 WP_112925650.1 Holliday junction resolvase RuvX -
  DCD74_RS00845 (DCD74_00845) - 181632..182540 (+) 909 WP_162615820.1 aspartate carbamoyltransferase catalytic subunit -
  DCD74_RS00850 (DCD74_00850) - 182559..183104 (-) 546 WP_112925652.1 Dps family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38515.23 Da        Isoelectric Point: 6.7805

>NTDB_id=293477 DCD74_RS00825 WP_112925647.1 178341..179378(+) (pilT) [Solilutibacter oculi strain 83-4]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPPLEHKTVHGLIYDIMSDKQRRDFEEFLETDFSFEIPGLAR
FRVNAFNHNRGAGAVFRTIPSEVLTLDQLGCPPLFRELVNQPQGLILVTGPTGSGKSTTLAAMLDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVIAQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVARSQITKQQAREYAKDKKLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=293477 DCD74_RS00825 WP_112925647.1 178341..179378(+) (pilT) [Solilutibacter oculi strain 83-4]
ATGGATATCGCAGAACTGTTGGCTTTCTCGGTCAAGAACAAGGCATCCGACTTGCACCTCTCGGCGGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGCATCAACATCCCGCCGCTCGAGCACAAGACCGTGCACGGCCTGATCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTTCGAGGAATTCCTCGAAACCGACTTCTCCTTCGAAATCCCGGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCACAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCGGAAGTGCTGACGCTCGA
CCAGCTCGGCTGCCCGCCGCTGTTCCGCGAGCTGGTGAACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGCTCGACCACATCAACAAGAACGAATACGGCCACATCCTCTCGGTCGAGGAC
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTCCACCGCGACACCCACGGCTTCAATGA
GGCGCTGCGTTCGGCGCTGCGTGAAGACCCGGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACGTCCAGCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGTTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATCGCGCAGGC
GCTGCTGAAGAAGGTGGGCGGCGGTCGTACCGCGGCCTGGGAAATCATGGTCGGCATCCCGGCCATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGAATGATGACCCTCGACCAGCACCTG
CAGGACCTGGTGGCGCGCAGCCAGATCACCAAGCAGCAGGCCCGCGAATACGCGAAGGACAAGAAGCTGTTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A344J315

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Pseudomonas aeruginosa PAK

78.171

98.261

0.768
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.71

0.762
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.955

97.101

0.67
  pilT Vibrio cholerae strain A1552

68.955

97.101

0.67
  pilT Neisseria meningitidis 8013

62.682

99.42

0.623
  pilT Neisseria gonorrhoeae MS11

62.391

99.42

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Pseudomonas stutzeri DSM 10701

40.118

98.261

0.394
  pilU Acinetobacter baylyi ADP1

39.118

98.551

0.386
  pilU Vibrio cholerae strain A1552

39.701

97.101

0.386

Multiple sequence alignment