Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DCD74_RS00830 Genome accession   NZ_CP029556
Coordinates   179421..180554 (+) Length   377 a.a.
NCBI ID   WP_112925648.1    Uniprot ID   A0A344J316
Organism   Solilutibacter oculi strain 83-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 174421..185554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCD74_RS00795 (DCD74_00795) - 174531..174887 (+) 357 WP_112925642.1 hypothetical protein -
  DCD74_RS00800 (DCD74_00800) - 174884..175708 (+) 825 WP_112925643.1 cobalamin-binding protein -
  DCD74_RS00805 (DCD74_00805) - 175678..176196 (-) 519 WP_237049676.1 GNAT family N-acetyltransferase -
  DCD74_RS00810 (DCD74_00810) - 176196..176684 (-) 489 WP_162615819.1 DUF4426 domain-containing protein -
  DCD74_RS00815 (DCD74_00815) proC 176687..177529 (-) 843 WP_112925645.1 pyrroline-5-carboxylate reductase -
  DCD74_RS00820 (DCD74_00820) - 177558..178184 (-) 627 WP_237049628.1 YggS family pyridoxal phosphate-dependent enzyme -
  DCD74_RS00825 (DCD74_00825) pilT 178341..179378 (+) 1038 WP_112925647.1 type IV pilus twitching motility protein PilT Machinery gene
  DCD74_RS00830 (DCD74_00830) pilU 179421..180554 (+) 1134 WP_112925648.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DCD74_RS00835 (DCD74_00835) - 180603..181169 (+) 567 WP_112925649.1 YqgE/AlgH family protein -
  DCD74_RS00840 (DCD74_00840) ruvX 181166..181630 (+) 465 WP_112925650.1 Holliday junction resolvase RuvX -
  DCD74_RS00845 (DCD74_00845) - 181632..182540 (+) 909 WP_162615820.1 aspartate carbamoyltransferase catalytic subunit -
  DCD74_RS00850 (DCD74_00850) - 182559..183104 (-) 546 WP_112925652.1 Dps family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41958.25 Da        Isoelectric Point: 6.7725

>NTDB_id=293478 DCD74_RS00830 WP_112925648.1 179421..180554(+) (pilU) [Solilutibacter oculi strain 83-4]
MTTPTEFTSLLKLMAAQRASDLFITAGMPPSMKVNGKLSPVTEEPLTPQQSRDLVLSVMTPPQREEFEKTHECNFAIGLS
GVGRFRVSAFYQRNQVGMVLRRIETKIPTVEELSLPPIIKQLAMTKRGIILFVGATGTGKTTSLASMIGHRNKNSTGHII
TIEDPIEFVHRHEGCIVTQREVGVDTDSWENALKNTLRQAPDVIMIGEIRTREGMDHAIAFAETGHLVLCTLHANNANQA
MDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLVPRSDGKGRRVAMEILLGTPLVQDYIRDGEVHKLKEVMKDSTQLGMKT
FDQSLFELYQAGEISYDDALRYADSQNEVRLKIKLAQSGDARTLAQGMDGVEVAEVK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=293478 DCD74_RS00830 WP_112925648.1 179421..180554(+) (pilU) [Solilutibacter oculi strain 83-4]
ATGACCACGCCCACCGAATTCACCTCGCTGCTCAAGCTGATGGCCGCGCAGCGCGCCTCCGACCTGTTCATCACCGCCGG
CATGCCGCCGTCGATGAAGGTCAACGGCAAGCTCTCGCCGGTCACCGAGGAGCCGCTCACGCCGCAGCAGAGCCGTGACC
TGGTGTTGTCGGTGATGACGCCGCCGCAGCGCGAGGAGTTCGAGAAGACCCATGAGTGCAACTTCGCGATCGGGCTGTCG
GGTGTGGGCCGCTTCCGTGTGTCCGCGTTCTACCAGCGCAATCAGGTGGGCATGGTGCTGCGCCGGATCGAGACCAAGAT
CCCGACGGTGGAAGAACTCAGCCTGCCGCCGATCATCAAGCAGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCG
GCGCCACCGGTACCGGCAAGACGACCTCGCTGGCGTCGATGATCGGCCACCGCAACAAGAACTCGACCGGCCACATCATC
ACCATCGAGGACCCGATCGAATTCGTGCACCGCCACGAGGGCTGCATCGTCACCCAGCGCGAGGTCGGCGTGGATACCGA
CAGCTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGATCCGGACCCGCGAAG
GCATGGACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCG
ATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGTTGCTGATGGATCTTTCGCTCAACCTCAAGGGCGT
GGTCGCGCAGCAGCTGGTGCCGCGTTCGGACGGCAAGGGCCGCCGGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGG
TGCAGGACTACATCCGCGACGGCGAGGTCCACAAGCTGAAGGAAGTGATGAAGGACTCCACCCAGCTCGGGATGAAGACC
TTCGACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGTTACGACGACGCGCTGCGCTACGCCGACTCGCAGAA
CGAGGTCCGCCTGAAGATCAAGCTGGCGCAGTCGGGCGATGCCCGCACGCTGGCGCAGGGCATGGATGGCGTGGAAGTCG
CCGAGGTCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A344J316

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.579

97.082

0.666
  pilU Acinetobacter baylyi ADP1

64.045

94.43

0.605
  pilU Vibrio cholerae strain A1552

53.803

94.164

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.318

93.369

0.414
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.143

92.838

0.363
  pilT Vibrio cholerae strain A1552

39.143

92.838

0.363
  pilT Acinetobacter nosocomialis M2

39.65

90.981

0.361
  pilT Acinetobacter baumannii D1279779

39.65

90.981

0.361
  pilT Acinetobacter baumannii strain A118

39.65

90.981

0.361

Multiple sequence alignment