Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DKK66_RS07440 Genome accession   NZ_CP029539
Coordinates   1572863..1573900 (+) Length   345 a.a.
NCBI ID   WP_045845898.1    Uniprot ID   -
Organism   Aquitalea sp. USM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1567863..1578900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKK66_RS07410 (DKK66_07415) aroG 1568324..1569400 (-) 1077 WP_062788989.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  DKK66_RS07415 (DKK66_07420) dksA 1569559..1569969 (-) 411 WP_045845893.1 RNA polymerase-binding protein DksA -
  DKK66_RS07420 (DKK66_07425) - 1570171..1570476 (-) 306 WP_062788988.1 c-type cytochrome -
  DKK66_RS07425 (DKK66_07430) - 1570551..1571132 (-) 582 WP_062788987.1 YggT family protein -
  DKK66_RS07430 (DKK66_07435) proC 1571139..1571942 (-) 804 WP_062788986.1 pyrroline-5-carboxylate reductase -
  DKK66_RS07435 (DKK66_07440) - 1572007..1572702 (-) 696 WP_062788985.1 YggS family pyridoxal phosphate-dependent enzyme -
  DKK66_RS07440 (DKK66_07445) pilT 1572863..1573900 (+) 1038 WP_045845898.1 type IV pilus twitching motility protein PilT Machinery gene
  DKK66_RS07445 (DKK66_07450) pilU 1573938..1575074 (+) 1137 WP_131356737.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DKK66_RS07450 (DKK66_07455) - 1575137..1576753 (+) 1617 WP_131356740.1 response regulator -
  DKK66_RS07455 (DKK66_07460) - 1576776..1577987 (-) 1212 WP_131356743.1 EAL and HDOD domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38370.99 Da        Isoelectric Point: 6.5047

>NTDB_id=293357 DKK66_RS07440 WP_045845898.1 1572863..1573900(+) (pilT) [Aquitalea sp. USM4]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDVRRINLPPMDHHDVHDMVYDIMNDYQRKIFEDEFECDFSFELPGIAR
FRVNAFVQNRGMGAVFRVIPSKVLTLEQLGAPKIFQDISSYPRGLVLVTGPTGSGKSTTLAAMVDYVNENNFSHILTVED
PIEFVHESKKSLINQRELGLQTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIRENKIAQINSMIQTGQQYGMQTLDQC
LQELVRRNIVAPSEARQKAASKDAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=293357 DKK66_RS07440 WP_045845898.1 1572863..1573900(+) (pilT) [Aquitalea sp. USM4]
ATGGAAATTTCCGAGCTCTTGGCCTTTACCGTCAAGAACAAGGCATCCGACTTGCACCTGTCGGCCGGTTTGCCTCCGAT
GATCCGGGTCAACGGCGATGTACGCCGTATCAACCTGCCCCCGATGGATCATCATGATGTACACGATATGGTGTACGACA
TCATGAACGACTATCAGCGCAAGATTTTCGAGGATGAGTTCGAATGCGACTTTTCCTTCGAACTGCCCGGCATCGCCCGC
TTCCGGGTAAATGCCTTTGTGCAGAACCGTGGCATGGGCGCGGTATTCCGTGTGATTCCGTCCAAGGTGCTGACGCTGGA
GCAACTGGGCGCACCCAAGATTTTCCAGGACATTTCCAGCTACCCGCGTGGCCTGGTACTGGTGACCGGCCCTACCGGTT
CGGGTAAGTCCACCACGCTGGCGGCCATGGTGGACTATGTGAACGAAAACAATTTTTCGCACATCCTCACCGTGGAAGAC
CCGATCGAATTCGTGCATGAAAGCAAGAAATCGCTGATCAACCAGCGTGAACTGGGTTTGCAGACCCACAGTTTTTCCAA
TGCCCTGCGTTCGGCCTTGCGTGAAGACCCGGACGTGATTCTGGTGGGTGAATTGCGCGATCTGGAAACCATCCGGCTGG
CGCTGACTGCGGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCATACCAGCTCGGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGGTGAAAAGGAAATGGTGCGCTCCATGCTGTCCGAATCGGTGCGGGCGGTGATTTCGCAAAC
CCTGCTCAAGACCAAGGATGGCAGTGGCCGGGTGGCTGCGCATGAAATCATGATCGGCACGCCGGCCATTCGTAACCTGA
TTCGCGAAAACAAGATTGCGCAGATCAACTCGATGATCCAGACCGGGCAGCAATACGGCATGCAAACGCTGGACCAGTGT
CTGCAGGAGCTGGTACGGCGCAATATTGTTGCCCCCAGCGAGGCACGCCAGAAAGCAGCGTCCAAGGATGCGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

74.203

100

0.742
  pilT Acinetobacter baumannii D1279779

74.203

100

0.742
  pilT Acinetobacter baumannii strain A118

74.203

100

0.742
  pilT Pseudomonas aeruginosa PAK

73.333

100

0.733
  pilT Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilT Acinetobacter baylyi ADP1

73.043

100

0.73
  pilT Neisseria meningitidis 8013

71.884

100

0.719
  pilT Neisseria gonorrhoeae MS11

71.594

100

0.716
  pilT Legionella pneumophila strain Lp02

69.565

100

0.696
  pilT Legionella pneumophila strain ERS1305867

69.565

100

0.696
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.971

0.664
  pilT Vibrio cholerae strain A1552

67.751

97.971

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Vibrio cholerae strain A1552

40.525

99.42

0.403
  pilU Pseudomonas stutzeri DSM 10701

42.284

93.913

0.397
  pilU Acinetobacter baylyi ADP1

39.181

99.13

0.388

Multiple sequence alignment