Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DKK66_RS07445 Genome accession   NZ_CP029539
Coordinates   1573938..1575074 (+) Length   378 a.a.
NCBI ID   WP_131356737.1    Uniprot ID   -
Organism   Aquitalea sp. USM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1568938..1580074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKK66_RS07415 (DKK66_07420) dksA 1569559..1569969 (-) 411 WP_045845893.1 RNA polymerase-binding protein DksA -
  DKK66_RS07420 (DKK66_07425) - 1570171..1570476 (-) 306 WP_062788988.1 c-type cytochrome -
  DKK66_RS07425 (DKK66_07430) - 1570551..1571132 (-) 582 WP_062788987.1 YggT family protein -
  DKK66_RS07430 (DKK66_07435) proC 1571139..1571942 (-) 804 WP_062788986.1 pyrroline-5-carboxylate reductase -
  DKK66_RS07435 (DKK66_07440) - 1572007..1572702 (-) 696 WP_062788985.1 YggS family pyridoxal phosphate-dependent enzyme -
  DKK66_RS07440 (DKK66_07445) pilT 1572863..1573900 (+) 1038 WP_045845898.1 type IV pilus twitching motility protein PilT Machinery gene
  DKK66_RS07445 (DKK66_07450) pilU 1573938..1575074 (+) 1137 WP_131356737.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DKK66_RS07450 (DKK66_07455) - 1575137..1576753 (+) 1617 WP_131356740.1 response regulator -
  DKK66_RS07455 (DKK66_07460) - 1576776..1577987 (-) 1212 WP_131356743.1 EAL and HDOD domain-containing protein -
  DKK66_RS07460 (DKK66_07465) - 1578240..1578974 (-) 735 WP_045845900.1 amino acid ABC transporter ATP-binding protein -
  DKK66_RS07465 (DKK66_07470) - 1578980..1579651 (-) 672 WP_045845901.1 ABC transporter permease subunit -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42351.52 Da        Isoelectric Point: 6.1381

>NTDB_id=293358 DKK66_RS07445 WP_131356737.1 1573938..1575074(+) (pilU) [Aquitalea sp. USM4]
MEKDQASKFIHDLLKHAVGKNASDIFISAEFPPAMKIDGKITPVAPQPLSAQHTKELVRSVMNDRQTEEFESKKEANFAI
NPPGIGRFRVSAFIQQGCAGMVLRKINTEIPTFDKLNMPEVLQDISLIKRGLVIFVGGTGSGKSTSLAALVDWRNSHNQD
HIITIEDPIEYVHQHKKCIVTQREVGVDTEDWEIALKNTLRQAPDVILMGEIRDRETMGYALQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDMSLNMRAIISQRLLPLKSGRGRVAAVEILLNTPLVSDMIFKGEVAGLKEVMSRSRESG
MQTFDQSLFDLFEAGYVSYEDALRNADSINDLRLKIKLYSESSKGRDPLDGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=293358 DKK66_RS07445 WP_131356737.1 1573938..1575074(+) (pilU) [Aquitalea sp. USM4]
ATGGAAAAAGACCAGGCATCAAAATTCATTCACGATCTGCTCAAGCATGCGGTGGGCAAGAACGCTTCGGACATCTTCAT
TTCGGCGGAATTTCCGCCCGCCATGAAGATTGACGGCAAGATCACCCCGGTGGCACCGCAACCTTTGTCGGCCCAGCACA
CCAAGGAGCTGGTGCGCTCGGTGATGAACGACCGGCAGACCGAAGAATTCGAGTCGAAAAAAGAGGCCAACTTTGCCATC
AACCCGCCGGGCATCGGCCGTTTCCGGGTGAGTGCCTTCATCCAGCAAGGCTGTGCCGGCATGGTGCTGCGCAAGATCAA
CACCGAAATCCCGACCTTCGACAAGCTGAACATGCCGGAGGTATTGCAGGATATTTCGCTGATCAAGCGTGGTCTGGTGA
TTTTTGTCGGCGGTACCGGTTCGGGCAAATCCACGTCGCTGGCAGCACTGGTGGACTGGCGCAACAGCCACAACCAGGAC
CACATCATCACCATCGAGGACCCGATCGAGTACGTGCATCAGCACAAGAAGTGCATCGTGACCCAGCGTGAAGTGGGGGT
GGATACCGAAGACTGGGAAATCGCCCTGAAAAACACCCTGCGTCAGGCACCGGATGTGATCCTGATGGGGGAAATCCGCG
ATCGGGAAACCATGGGCTACGCCTTGCAGTTTGCCGAAACCGGCCACCTGTGCCTGGCTACCCTGCATGCCAACAATGCC
AACCAGGCGCTGGACCGGATTCTGAACTTCTTCCCGGAAGAACGTCACCAGCAAGTGCTGATGGATATGTCGCTGAACAT
GCGCGCCATCATTTCGCAGCGCCTGCTGCCGCTCAAGTCGGGCCGCGGCCGGGTGGCGGCAGTGGAAATCCTGCTCAATA
CCCCGCTGGTTTCCGACATGATCTTCAAGGGCGAGGTGGCCGGGCTCAAGGAGGTGATGAGCCGCTCGCGTGAGTCGGGC
ATGCAAACCTTCGACCAGTCGCTGTTCGACCTGTTTGAAGCCGGCTATGTCAGCTACGAGGATGCGCTGCGCAATGCGGA
CTCCATCAACGACCTGCGCCTGAAAATCAAACTGTATAGCGAAAGCTCCAAGGGACGTGACCCGCTGGATGGCATCGACC
ATCTGGATATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62

92.593

0.574
  pilU Acinetobacter baylyi ADP1

56.787

95.503

0.542
  pilU Vibrio cholerae strain A1552

51.639

96.825

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas aeruginosa PAK

42.73

89.153

0.381
  pilT Vibrio cholerae strain A1552

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

41.888

89.683

0.376
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376
  pilT Acinetobacter baumannii strain A118

42.342

88.095

0.373
  pilT Acinetobacter nosocomialis M2

42.342

88.095

0.373
  pilT Acinetobacter baumannii D1279779

42.342

88.095

0.373
  pilT Neisseria meningitidis 8013

40.882

89.947

0.368

Multiple sequence alignment