Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DJ533_RS15145 Genome accession   NZ_CP029397
Coordinates   2650054..2651172 (+) Length   372 a.a.
NCBI ID   WP_065994225.1    Uniprot ID   A0A2S2FFP1
Organism   Acinetobacter defluvii strain WCHA30     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2645054..2656172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ533_RS15120 (DJ533_15155) - 2646058..2646780 (+) 723 WP_065994221.1 folate-binding protein YgfZ -
  DJ533_RS15125 (DJ533_15160) - 2646860..2647852 (+) 993 WP_065994222.1 hypothetical protein -
  DJ533_RS15130 (DJ533_15165) - 2647890..2648107 (-) 218 Protein_2464 glucose/sorbosone dehydrogenase -
  DJ533_RS15135 (DJ533_15170) - 2648182..2648868 (-) 687 WP_065994223.1 YggS family pyridoxal phosphate-dependent enzyme -
  DJ533_RS15140 (DJ533_15175) pilT 2648990..2650027 (+) 1038 WP_065994224.1 type IV pilus twitching motility protein PilT Machinery gene
  DJ533_RS15145 (DJ533_15180) pilU 2650054..2651172 (+) 1119 WP_065994225.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DJ533_RS15150 (DJ533_15185) fur 2651253..2651690 (-) 438 WP_065994226.1 ferric iron uptake transcriptional regulator -
  DJ533_RS15155 (DJ533_15190) - 2651803..2652201 (+) 399 WP_065994227.1 outer membrane protein assembly factor BamE -
  DJ533_RS15160 (DJ533_15195) - 2652285..2652629 (-) 345 WP_065994228.1 RnfH family protein -
  DJ533_RS15165 (DJ533_15200) - 2652629..2653693 (-) 1065 WP_065994229.1 hypothetical protein -
  DJ533_RS15170 (DJ533_15205) - 2653846..2654301 (-) 456 WP_065994230.1 bacteriohemerythrin -
  DJ533_RS15175 (DJ533_15210) - 2654681..2655529 (-) 849 WP_065994231.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41784.06 Da        Isoelectric Point: 6.3492

>NTDB_id=292234 DJ533_RS15145 WP_065994225.1 2650054..2651172(+) (pilU) [Acinetobacter defluvii strain WCHA30]
MDFNDLLNMMIEQKASDLFITADVEPSMKINSQIVPVVKTKLTGELVGQIIHSIMTDKQRKEFADTRECNFAIVNKDKTA
RFRVSAFQQRDQPGMILRRIETTIPTMEDLKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASLIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANNKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=292234 DJ533_RS15145 WP_065994225.1 2650054..2651172(+) (pilU) [Acinetobacter defluvii strain WCHA30]
ATGGATTTTAATGATTTGCTCAACATGATGATTGAGCAAAAAGCATCGGATTTGTTTATTACTGCCGATGTTGAACCATC
AATGAAAATTAATAGTCAAATTGTACCTGTGGTCAAAACCAAATTAACTGGAGAGTTAGTTGGGCAGATCATTCATTCGA
TTATGACGGATAAACAGCGCAAAGAATTTGCCGACACACGGGAATGTAACTTTGCGATTGTGAATAAAGACAAAACCGCA
CGTTTCCGTGTCAGCGCATTTCAACAACGTGACCAACCTGGAATGATTTTACGTCGTATCGAAACTACGATTCCGACGAT
GGAAGATCTAAAACTGCCACCGATTTTAAAAGATCTTGCGATGACCAAACGTGGCATCATCATTTTTGTTGGGGCGACAG
GTACAGGTAAATCGACCTCTTTGGCATCACTGATTGGTTATCGAAATCAAAATTCCAAAGGGCATATCATTACCATCGAA
GATCCGATTGAGTTTGTGCATGAACATGCAGGCTGTATTATCACGCAGCGTGAAGTCGGTATTGATACGGATTCTTTTGA
AATCGCACTAAAAAATACCTTACGTCAAGCGCCTGATGTGATTTTAATCGGTGAGATTCGCTCTCGTGAAACCATGGATT
ATGCCATTGCCTTTGCTGAAACGGGGCACTTGGTTTTTGCAACCCTACATGCCAACAACGCTAACCAAGCGATTGACCGT
ATTATTCACTTCTTTGAGTCCGATCGGCATAGCCAATTGTTTATGGACTTATCCTTAAACTTAAAAGCAATGATTGCACA
GCAATTGATTCCAACACCCGATGGTAATTCACGCCGTGCTGCTATTGAGATTCTCATTAACTCACCTTTAATTGCTGATT
TAATTCGTAAAGGTGATGTACATGAAATCAAAGATTTGATGAAGCGCTCACGTGAACTGGGTATGCAGACATTTGACCAA
GCACTCTTTGATCTCTATAAAGCAGGTCAAATTACCTATAAAGATGCACTGAAACACGCAGACTCACCAAACGACTTACG
TCTACAAATTAAATTAGATGAAGAAGGTGGCGCACAGTTGTTAAATGCCAACAACAAACTTACGTTCGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2FFP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.132

100

0.763
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.731

0.677
  pilU Vibrio cholerae strain A1552

55.342

98.118

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.618

93.011

0.387
  pilT Legionella pneumophila strain Lp02

41.716

90.86

0.379
  pilT Legionella pneumophila strain ERS1305867

41.716

90.86

0.379
  pilT Acinetobacter nosocomialis M2

40.233

92.204

0.371
  pilT Acinetobacter baumannii D1279779

40.233

92.204

0.371
  pilT Acinetobacter baumannii strain A118

40.233

92.204

0.371
  pilT Pseudomonas aeruginosa PAK

39.595

93.011

0.368
  pilT Acinetobacter baylyi ADP1

39.65

92.204

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.306

93.011

0.366
  pilT Neisseria meningitidis 8013

39.306

93.011

0.366
  pilT Neisseria gonorrhoeae MS11

39.306

93.011

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.483

88.978

0.36
  pilT Vibrio cholerae strain A1552

40.483

88.978

0.36

Multiple sequence alignment