Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DJ533_RS15140 Genome accession   NZ_CP029397
Coordinates   2648990..2650027 (+) Length   345 a.a.
NCBI ID   WP_065994224.1    Uniprot ID   A0A2S2FFX5
Organism   Acinetobacter defluvii strain WCHA30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2643990..2655027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ533_RS15120 (DJ533_15155) - 2646058..2646780 (+) 723 WP_065994221.1 folate-binding protein YgfZ -
  DJ533_RS15125 (DJ533_15160) - 2646860..2647852 (+) 993 WP_065994222.1 hypothetical protein -
  DJ533_RS15130 (DJ533_15165) - 2647890..2648107 (-) 218 Protein_2464 glucose/sorbosone dehydrogenase -
  DJ533_RS15135 (DJ533_15170) - 2648182..2648868 (-) 687 WP_065994223.1 YggS family pyridoxal phosphate-dependent enzyme -
  DJ533_RS15140 (DJ533_15175) pilT 2648990..2650027 (+) 1038 WP_065994224.1 type IV pilus twitching motility protein PilT Machinery gene
  DJ533_RS15145 (DJ533_15180) pilU 2650054..2651172 (+) 1119 WP_065994225.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DJ533_RS15150 (DJ533_15185) fur 2651253..2651690 (-) 438 WP_065994226.1 ferric iron uptake transcriptional regulator -
  DJ533_RS15155 (DJ533_15190) - 2651803..2652201 (+) 399 WP_065994227.1 outer membrane protein assembly factor BamE -
  DJ533_RS15160 (DJ533_15195) - 2652285..2652629 (-) 345 WP_065994228.1 RnfH family protein -
  DJ533_RS15165 (DJ533_15200) - 2652629..2653693 (-) 1065 WP_065994229.1 hypothetical protein -
  DJ533_RS15170 (DJ533_15205) - 2653846..2654301 (-) 456 WP_065994230.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38319.86 Da        Isoelectric Point: 6.5051

>NTDB_id=292233 DJ533_RS15140 WP_065994224.1 2648990..2650027(+) (pilT) [Acinetobacter defluvii strain WCHA30]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPAFEHKDVHRLVYDIMNDKQRRDYEEQLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGVPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGTISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=292233 DJ533_RS15140 WP_065994224.1 2648990..2650027(+) (pilT) [Acinetobacter defluvii strain WCHA30]
ATGGATATTACAGAATTACTAGCTTTTTCCGTTAAAAACGGTGCATCAGATTTACACTTATCAGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAGGTTCGTCGCATCAACCTTCCTGCTTTCGAACATAAAGATGTACATCGTCTTGTTTATGACA
TCATGAATGATAAACAGCGTCGTGATTATGAAGAACAACTGGAAACTGACTTTTCATTTGAAGTACCTGGCGTCGCACGT
TTCCGTGTCAACGCATTTAACCAAAACCGGGGTGCTGGTGCGGTATTTCGTACCATTCCATCCAAAGTATTAACGATGGA
AGATTTAGGTCTGGGACAAATCTTTAAAGATATTTGTGATTATCCACGTGGAATTGTGTTAGTGACAGGTCCTACAGGTT
CTGGTAAATCCACCACATTGGCAGCCATGCTGGATTATATCAACGACAACCGCTATGACCATATCTTAACAGTTGAAGAC
CCAATCGAGTTTGTACATCAGTCTAAAAAATGTCTGATCAACCAGCGTGAAGTACATCGTGATACACACGGTTTTAATGA
AGCACTCCGTTCAGCGCTGCGTGAAGACCCTGATATTATCTTGGTTGGTGAGATGCGTGACTTAGAAACGATTCGCTTGG
CGCTTACTGCGGCGGAAACAGGTCACTTGGTGTTTGGTACACTGCATACTACCTCTGCGGCAAAAACGATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTACGTGCCATGCTCTCTGAGTCTTTACAAGCGGTAATCTCACAGAC
GCTTTTAAAGAAAAATGGCGGTGGTCGTGTAGCAGCTCATGAGATCATGATCGGTGTACCTGCGATTCGTAACTTGATTC
GTGAAAACAAAGTAGCACAGATGTATTCAGCCATTCAAACCGGTGCAAACTATGGTATGACGACATTGGATCAGAGCTTA
AAGACTTTGGTATCCAAAGGCACGATCAGTCCACAAGTAGCACGTACTGCGGCAAAACAGCCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2FFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

97.101

100

0.971
  pilT Acinetobacter baumannii D1279779

96.812

100

0.968
  pilT Acinetobacter baumannii strain A118

96.812

100

0.968
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.084

96.232

0.684
  pilT Vibrio cholerae strain A1552

71.084

96.232

0.684
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.942

0.394
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386

Multiple sequence alignment