Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SSED_RS06340 Genome accession   NC_009831
Coordinates   1466350..1467384 (+) Length   344 a.a.
NCBI ID   WP_012141580.1    Uniprot ID   A8FSM1
Organism   Shewanella sediminis HAW-EB3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1461350..1472384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SSED_RS06310 (Ssed_1227) rdgB 1462438..1463040 (-) 603 WP_012141574.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SSED_RS06315 (Ssed_1228) - 1463240..1463674 (-) 435 WP_012141575.1 DUF4426 domain-containing protein -
  SSED_RS06320 (Ssed_1229) yggU 1463777..1464064 (-) 288 WP_012141576.1 DUF167 family protein YggU -
  SSED_RS06325 (Ssed_1230) - 1464064..1464612 (-) 549 WP_012141577.1 YggT family protein -
  SSED_RS06330 (Ssed_1231) proC 1464657..1465475 (-) 819 WP_012141578.1 pyrroline-5-carboxylate reductase -
  SSED_RS06335 (Ssed_1232) - 1465609..1466313 (-) 705 WP_012141579.1 YggS family pyridoxal phosphate-dependent enzyme -
  SSED_RS06340 (Ssed_1233) pilT 1466350..1467384 (+) 1035 WP_012141580.1 type IV pilus twitching motility protein PilT Machinery gene
  SSED_RS06345 (Ssed_1234) pilU 1467411..1468523 (+) 1113 WP_012141581.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SSED_RS06350 (Ssed_1235) hemH 1468858..1469895 (+) 1038 WP_012141582.1 ferrochelatase -
  SSED_RS06355 (Ssed_1236) ruvX 1469964..1470383 (-) 420 WP_012141583.1 Holliday junction resolvase RuvX -
  SSED_RS06360 (Ssed_1237) - 1470468..1471028 (-) 561 WP_012141584.1 YqgE/AlgH family protein -
  SSED_RS06365 (Ssed_1238) yciH 1471075..1471404 (-) 330 WP_012141585.1 stress response translation initiation inhibitor YciH -
  SSED_RS06370 (Ssed_1239) - 1471938..1472255 (-) 318 WP_012141586.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38216.81 Da        Isoelectric Point: 6.9449

>NTDB_id=29198 SSED_RS06340 WP_012141580.1 1466350..1467384(+) (pilT) [Shewanella sediminis HAW-EB3]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHGLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQARGAAAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMIDYINDARHEHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQITREDAQHKSATKQTF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=29198 SSED_RS06340 WP_012141580.1 1466350..1467384(+) (pilT) [Shewanella sediminis HAW-EB3]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAGCACAAAGCGTCAGATCTACACCTTTCAGCGGGCGTTTCGCCTAT
GATACGTGTTGATGGCGAAGTCAGGAAGATCAATTTACCCGCTTTAGATCATCAAGGTGTTCATGGACTCGTCTATGACA
TCATGAATGATAAGCAGCGTAAAGATTATGAAGAACATTTAGAGATAGATTTTTCGTTCGAAGTGCCAAATTTAGCGCGT
TTTCGTGTCAATGCCTTCAATCAGGCTCGAGGTGCTGCGGCCGTATTCCGTACTATCCCCAGTGACATCTTAAGCCTTGA
GCAACTCGGCGCGCCGGAGATTTTTAAGAAGATCTCCAGCTTTCCCCGTGGGCTTGTGTTGGTAACCGGACCAACAGGTT
CGGGTAAAAGTACTACCTTAGCGGCCATGATCGATTATATAAACGATGCTCGTCACGAGCATATTCTGACCATCGAAGAC
CCGATAGAATTCGTTCACCAAAATAAGCAATGCCTGATTAACCAGCGTGAGGTTCACCGCCATACTCACAGCTTTAATGC
GGCTTTGCGTAGTGCACTTCGTGAAGATCCCGATGTTATTCTGGTGGGTGAGATGCGAGATCTCGAGACTATTCGTCTTG
CGATGACTGCGGCCGAGACGGGTCATCTGGTTTTCGGCACCTTGCATACCACGTCGGCGGCTAAAACCATTGACCGTATA
GTCGATGTGTTCCCCGAAGGAGAAAAGGGCATGGTCAGAACTATGTTGTCTGAGTCGCTACAGGCGGTAATTTCTCAAAC
CCTGATTAAGAAGGTGGGTGGCGGCCGTGTCGCAGCTCACGAGATCATGATGGGAACCCCGGCGATTCGTAACCTTATTC
GAGAAGACAAAGTCGCTCAAATGTATTCAGCGATTCAAACGGGTATGGCTCACGGTATGCAGACCTTAGATCAATGCCTG
CAGAATCTGGTTAATCGTGGTCAAATTACTCGTGAAGATGCACAACATAAGAGTGCAACCAAGCAAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FSM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Pseudomonas aeruginosa PAK

77.326

100

0.773
  pilT Pseudomonas stutzeri DSM 10701

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

75

100

0.75
  pilT Legionella pneumophila strain ERS1305867

75

100

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.997

97.965

0.715
  pilT Vibrio cholerae strain A1552

72.997

97.965

0.715
  pilT Neisseria meningitidis 8013

68.986

100

0.692
  pilT Neisseria gonorrhoeae MS11

68.696

100

0.689
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

41.593

98.547

0.41
  pilU Acinetobacter baylyi ADP1

40.476

97.674

0.395

Multiple sequence alignment