Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SSED_RS06345 Genome accession   NC_009831
Coordinates   1467411..1468523 (+) Length   370 a.a.
NCBI ID   WP_012141581.1    Uniprot ID   A8FSM2
Organism   Shewanella sediminis HAW-EB3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1462411..1473523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SSED_RS06310 (Ssed_1227) rdgB 1462438..1463040 (-) 603 WP_012141574.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SSED_RS06315 (Ssed_1228) - 1463240..1463674 (-) 435 WP_012141575.1 DUF4426 domain-containing protein -
  SSED_RS06320 (Ssed_1229) yggU 1463777..1464064 (-) 288 WP_012141576.1 DUF167 family protein YggU -
  SSED_RS06325 (Ssed_1230) - 1464064..1464612 (-) 549 WP_012141577.1 YggT family protein -
  SSED_RS06330 (Ssed_1231) proC 1464657..1465475 (-) 819 WP_012141578.1 pyrroline-5-carboxylate reductase -
  SSED_RS06335 (Ssed_1232) - 1465609..1466313 (-) 705 WP_012141579.1 YggS family pyridoxal phosphate-dependent enzyme -
  SSED_RS06340 (Ssed_1233) pilT 1466350..1467384 (+) 1035 WP_012141580.1 type IV pilus twitching motility protein PilT Machinery gene
  SSED_RS06345 (Ssed_1234) pilU 1467411..1468523 (+) 1113 WP_012141581.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SSED_RS06350 (Ssed_1235) hemH 1468858..1469895 (+) 1038 WP_012141582.1 ferrochelatase -
  SSED_RS06355 (Ssed_1236) ruvX 1469964..1470383 (-) 420 WP_012141583.1 Holliday junction resolvase RuvX -
  SSED_RS06360 (Ssed_1237) - 1470468..1471028 (-) 561 WP_012141584.1 YqgE/AlgH family protein -
  SSED_RS06365 (Ssed_1238) yciH 1471075..1471404 (-) 330 WP_012141585.1 stress response translation initiation inhibitor YciH -
  SSED_RS06370 (Ssed_1239) - 1471938..1472255 (-) 318 WP_012141586.1 hypothetical protein -
  SSED_RS06375 (Ssed_1240) - 1472427..1472987 (+) 561 WP_012141587.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41043.06 Da        Isoelectric Point: 5.8282

>NTDB_id=29199 SSED_RS06345 WP_012141581.1 1467411..1468523(+) (pilU) [Shewanella sediminis HAW-EB3]
MDVRPFLKIMVERKASDLFITAGFPPSAKIDGELRPLSESSFTPAQALDFVESLMTDVQKTEFHESRECNFAFAAKELGR
FRVSAFWQREAPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNANSRGHILTIED
PVEFVHEHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRISSLIAKNELHSLKETMAKSNEQGMQTFDQA
LLKLYSEGEISYADALHYADSPNDLRLMIKLQGSDPSSSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=29199 SSED_RS06345 WP_012141581.1 1467411..1468523(+) (pilU) [Shewanella sediminis HAW-EB3]
ATGGATGTTCGTCCCTTCTTAAAAATAATGGTGGAGCGTAAAGCATCGGATCTATTTATCACTGCAGGCTTCCCACCCAG
TGCAAAAATTGATGGTGAGTTGAGACCCTTAAGTGAGAGTTCATTTACCCCCGCACAGGCATTGGATTTTGTTGAATCTT
TGATGACGGATGTGCAGAAAACGGAGTTTCATGAGAGTCGGGAGTGTAACTTTGCTTTTGCCGCAAAAGAGCTGGGTCGT
TTTCGTGTCAGCGCCTTCTGGCAAAGAGAAGCGCCGGGCTGTGTCATGCGCAGGATTGAAACTAAAATTCCGGAAGTGGA
AGATCTGAAGCTCCCACCCATTTTAAAAGACCTCGTGATGAGTAAACGCGGGCTTATCATCATGGTCGGTGGTACGGGAA
CGGGTAAATCGACCTCGTTGGCGGCGCTGGTGGGTTATCGTAATGCAAACTCTCGAGGTCATATCCTGACAATTGAGGAT
CCCGTCGAGTTTGTTCACGAACACCGCAAGAGTATTATTACTCAGCGAGAAGTCGGTATCGATACCGAGTCATTCGATGC
CGCCCTTAAAAGTTCGCTTCGTCAGGCTCCGGATGTGATCTTAATCGGTGAGATCCGAAGCCAGGAGACGATGGAGTTTG
CACTCTCATTTGCAGAAACCGGTCACCTGTGTATGGCAACGTTGCACGCAAACAATGCAAACCAAGCACTGGACCGTATC
ATGCACTTAGTGCCGGAGAGTAAGCATCAACAGCTGTTATTCGACCTTTCATTAAACTTACGAGGTATCGTCGCACAACA
GTTGGTTCCCAAAGCGGACGGCACTGGTCGACGTGCAGCCATTGAAATTTTGATCAACACGCCAAGGATCTCAAGTTTGA
TTGCGAAAAATGAGCTGCACTCGCTAAAAGAGACGATGGCTAAGTCAAACGAGCAGGGGATGCAGACTTTCGATCAAGCG
TTACTCAAACTCTATAGTGAAGGGGAGATAAGTTACGCCGATGCGCTTCATTACGCCGATTCACCTAATGATCTTCGCTT
GATGATTAAACTTCAAGGCTCTGACCCCTCCAGTTCAGGTTTCATGGAAGGGGTTACACTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FSM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Acinetobacter baylyi ADP1

61.364

95.135

0.584
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

94.054

0.392
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.03

89.189

0.384
  pilT Vibrio cholerae strain A1552

43.03

89.189

0.384
  pilT Acinetobacter nosocomialis M2

40.936

92.432

0.378
  pilT Legionella pneumophila strain ERS1305867

41.994

89.459

0.376
  pilT Legionella pneumophila strain Lp02

41.994

89.459

0.376
  pilT Acinetobacter baumannii D1279779

40.643

92.432

0.376
  pilT Acinetobacter baumannii strain A118

40.643

92.432

0.376
  pilT Acinetobacter baylyi ADP1

39.766

92.432

0.368
  pilT Pseudomonas aeruginosa PAK

39.13

93.243

0.365
  pilT Pseudomonas stutzeri DSM 10701

38.841

93.243

0.362

Multiple sequence alignment