Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HMF8227_RS12035 Genome accession   NZ_CP029347
Coordinates   2563406..2564443 (-) Length   345 a.a.
NCBI ID   WP_109340408.1    Uniprot ID   A0A2S2E5E2
Organism   Saliniradius amylolyticus strain HMF8227     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2558406..2569443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMF8227_RS12005 (HMF8227_02416) gshB 2559269..2560198 (+) 930 WP_420820557.1 glutathione synthase -
  HMF8227_RS12010 (HMF8227_02417) - 2560218..2560775 (+) 558 WP_109340404.1 YqgE/AlgH family protein -
  HMF8227_RS12015 (HMF8227_02418) ruvX 2560779..2561195 (+) 417 WP_109340405.1 Holliday junction resolvase RuvX -
  HMF8227_RS12020 (HMF8227_02419) - 2561231..2561986 (+) 756 WP_109340406.1 substrate-binding periplasmic protein -
  HMF8227_RS12025 (HMF8227_02420) - 2561931..2562251 (-) 321 WP_239421301.1 DUF2007 domain-containing protein -
  HMF8227_RS12030 (HMF8227_02421) pilU 2562252..2563364 (-) 1113 WP_109340407.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HMF8227_RS12035 (HMF8227_02422) pilT 2563406..2564443 (-) 1038 WP_109340408.1 type IV pilus twitching motility protein PilT Machinery gene
  HMF8227_RS12040 (HMF8227_02423) - 2564554..2565240 (+) 687 WP_109340409.1 YggS family pyridoxal phosphate-dependent enzyme -
  HMF8227_RS12045 (HMF8227_02424) proC 2565251..2566099 (+) 849 WP_275425516.1 pyrroline-5-carboxylate reductase -
  HMF8227_RS12050 (HMF8227_02425) - 2566103..2566648 (+) 546 WP_109340411.1 YggT family protein -
  HMF8227_RS12055 (HMF8227_02426) - 2566818..2567243 (+) 426 WP_109340412.1 DUF4426 domain-containing protein -
  HMF8227_RS12060 (HMF8227_02427) - 2567317..2568108 (+) 792 WP_338056574.1 substrate-binding periplasmic protein -
  HMF8227_RS12065 (HMF8227_02428) yaaA 2568173..2568952 (-) 780 WP_109340414.1 peroxide stress protein YaaA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38277.06 Da        Isoelectric Point: 6.3473

>NTDB_id=291842 HMF8227_RS12035 WP_109340408.1 2563406..2564443(-) (pilT) [Saliniradius amylolyticus strain HMF8227]
MDITELLAFSIKNNASDLHLSAGLPPMIRVDGDMRRLNVPEMDDKQVHNLIYEIMNDKQIKEYEENLETDFSFEVTGLCR
FRVNAFNQNRGPAAVLRTIPSKVLTLDDLGAPEIFKKIIDQPTGIVLVTGATGSGKSTTLAAMIDHINTNKRQHILTIED
PIEFVHDNKMSLVNQREVHRDTQGFEKALRSALREDPDVILVGELRDLETIRLAITAAETGHLVFATLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAGHEIMVGIPAIRNLIREDKVPQMYSVIQTGQSHGMQTMDQCL
QKLVAAGQITPQDAAAKAVDKQPKF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=291842 HMF8227_RS12035 WP_109340408.1 2563406..2564443(-) (pilT) [Saliniradius amylolyticus strain HMF8227]
GTGGATATTACCGAACTACTCGCCTTTAGCATTAAAAATAATGCCTCTGACTTGCATCTTTCTGCTGGTCTGCCGCCGAT
GATCCGTGTGGACGGCGATATGCGTCGCCTGAATGTACCGGAAATGGACGATAAGCAGGTACATAATCTGATCTACGAGA
TCATGAACGACAAACAGATCAAGGAATATGAGGAAAACCTGGAAACAGATTTCTCTTTCGAAGTCACCGGCTTATGTCGT
TTCCGGGTCAATGCCTTTAATCAGAACCGAGGTCCTGCAGCGGTGCTCAGGACCATTCCTAGTAAGGTATTGACTCTGGA
TGATCTGGGCGCGCCGGAGATATTTAAAAAAATCATCGACCAGCCCACAGGTATTGTGTTGGTCACGGGGGCGACCGGCT
CGGGTAAGAGTACGACCCTGGCAGCTATGATTGATCATATCAACACCAACAAACGCCAGCATATTCTGACCATCGAAGAT
CCCATCGAATTTGTGCACGACAACAAGATGAGCCTGGTCAACCAGCGAGAGGTTCATCGGGACACTCAGGGGTTTGAAAA
GGCCCTGCGCTCGGCGCTACGGGAAGACCCCGATGTGATTTTGGTGGGGGAGCTTCGGGATCTGGAAACCATTCGTCTGG
CCATCACGGCAGCGGAAACCGGTCACCTGGTATTTGCTACACTGCATACCAACTCAGCCCCCAAAACCATCGACAGGATT
ATCGATGTTTTCCCGGCCGAGGAGAAGGCCATGGTGCGCTCCATGCTGTCAGAGTCATTGCGCGCGGTCATCTCCCAGAC
CTTGCTTAAGAAGGTGGGTGGCGGACGTGTTGCCGGCCACGAGATTATGGTGGGCATACCGGCGATTCGAAATCTCATCC
GTGAAGACAAGGTGCCGCAGATGTACTCGGTGATTCAGACCGGTCAGTCCCACGGTATGCAGACGATGGATCAGTGCCTG
CAGAAGCTGGTGGCTGCGGGACAGATTACGCCTCAGGATGCGGCCGCTAAAGCGGTGGATAAGCAGCCTAAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2E5E2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.71

0.768
  pilT Acinetobacter baumannii strain A118

75.581

99.71

0.754
  pilT Acinetobacter baumannii D1279779

75.581

99.71

0.754
  pilT Acinetobacter nosocomialis M2

75.291

99.71

0.751
  pilT Pseudomonas aeruginosa PAK

76.331

97.971

0.748
  pilT Acinetobacter baylyi ADP1

74.419

99.71

0.742
  pilT Legionella pneumophila strain Lp02

69.795

98.841

0.69
  pilT Legionella pneumophila strain ERS1305867

69.795

98.841

0.69
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.681

0.678
  pilT Vibrio cholerae strain A1552

69.436

97.681

0.678
  pilT Neisseria meningitidis 8013

67.055

99.42

0.667
  pilT Neisseria gonorrhoeae MS11

66.764

99.42

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.603

99.42

0.513
  pilU Pseudomonas stutzeri DSM 10701

41.888

98.261

0.412
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4

Multiple sequence alignment