Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HMF8227_RS12030 Genome accession   NZ_CP029347
Coordinates   2562252..2563364 (-) Length   370 a.a.
NCBI ID   WP_109340407.1    Uniprot ID   A0A2S2E5G2
Organism   Saliniradius amylolyticus strain HMF8227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2557252..2568364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMF8227_RS12000 (HMF8227_02415) - 2558076..2559155 (+) 1080 WP_109340402.1 hypothetical protein -
  HMF8227_RS12005 (HMF8227_02416) gshB 2559269..2560198 (+) 930 WP_420820557.1 glutathione synthase -
  HMF8227_RS12010 (HMF8227_02417) - 2560218..2560775 (+) 558 WP_109340404.1 YqgE/AlgH family protein -
  HMF8227_RS12015 (HMF8227_02418) ruvX 2560779..2561195 (+) 417 WP_109340405.1 Holliday junction resolvase RuvX -
  HMF8227_RS12020 (HMF8227_02419) - 2561231..2561986 (+) 756 WP_109340406.1 substrate-binding periplasmic protein -
  HMF8227_RS12025 (HMF8227_02420) - 2561931..2562251 (-) 321 WP_239421301.1 DUF2007 domain-containing protein -
  HMF8227_RS12030 (HMF8227_02421) pilU 2562252..2563364 (-) 1113 WP_109340407.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HMF8227_RS12035 (HMF8227_02422) pilT 2563406..2564443 (-) 1038 WP_109340408.1 type IV pilus twitching motility protein PilT Machinery gene
  HMF8227_RS12040 (HMF8227_02423) - 2564554..2565240 (+) 687 WP_109340409.1 YggS family pyridoxal phosphate-dependent enzyme -
  HMF8227_RS12045 (HMF8227_02424) proC 2565251..2566099 (+) 849 WP_275425516.1 pyrroline-5-carboxylate reductase -
  HMF8227_RS12050 (HMF8227_02425) - 2566103..2566648 (+) 546 WP_109340411.1 YggT family protein -
  HMF8227_RS12055 (HMF8227_02426) - 2566818..2567243 (+) 426 WP_109340412.1 DUF4426 domain-containing protein -
  HMF8227_RS12060 (HMF8227_02427) - 2567317..2568108 (+) 792 WP_338056574.1 substrate-binding periplasmic protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40890.80 Da        Isoelectric Point: 5.1432

>NTDB_id=291841 HMF8227_RS12030 WP_109340407.1 2562252..2563364(-) (pilU) [Saliniradius amylolyticus strain HMF8227]
MQLAPYLKTATDKDASDIFITVGFPVSAKINGELTSLGGDKLSKDDAQALVHSVMEERYIKELDETKECNFAVGIDGVGR
FRCSAFWQRDSIGMVVRRIVTEIPKADDLGLPPILKDIIMSKRGLVLFVGATGTGKSTSLAALIGHRNHHSRGHILTIED
PVEFIHQHDNCMVTQREVGVDTNTFDDALKSSLRQAPDMILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERI
MHLAPPEQHEKLRFDLALNMRAIVAQQLVPTADGKGRVAAIEVLLNSPLVSDLIARNDIGELKEVMQKSQEMGMQTFDMA
LYQLYKEQKITKDQALHHADSPNDLRLMIKLQSDDGPDLGSLSDVSIDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=291841 HMF8227_RS12030 WP_109340407.1 2562252..2563364(-) (pilU) [Saliniradius amylolyticus strain HMF8227]
ATGCAACTGGCTCCTTATTTAAAAACCGCCACCGATAAAGATGCTTCGGATATCTTTATTACCGTGGGTTTTCCGGTGAG
TGCCAAGATTAACGGTGAACTGACGTCCTTAGGTGGCGATAAGCTCAGTAAAGATGATGCCCAGGCGCTCGTTCACAGCG
TGATGGAAGAGCGTTACATTAAAGAGTTGGATGAGACCAAAGAATGTAATTTTGCGGTGGGCATCGATGGTGTTGGGCGC
TTCCGTTGCAGTGCCTTCTGGCAAAGAGATTCCATTGGTATGGTGGTGCGCCGGATTGTCACCGAGATCCCGAAAGCCGA
CGATTTGGGCCTGCCGCCAATCCTCAAAGACATCATCATGAGCAAACGCGGCCTGGTGTTGTTTGTGGGGGCCACCGGTA
CCGGTAAGTCCACATCACTGGCGGCCCTGATCGGCCACCGAAACCACCATTCCCGCGGCCATATTCTGACTATTGAGGAC
CCCGTGGAGTTTATTCACCAGCACGACAACTGCATGGTGACCCAACGGGAAGTGGGCGTCGATACCAATACCTTCGATGA
CGCTTTGAAAAGCAGTTTGCGTCAGGCTCCGGATATGATCCTAATCGGTGAGATTCGCTCCATGGAGACTATGGAGTACG
CCATGTCCTTTGCCGATACCGGCCATCTGTGTGTGGCCACCTTGCACGCCAATAATGCCAACCAGGCCATAGAACGAATT
ATGCATCTGGCACCACCGGAGCAACATGAAAAACTGCGTTTTGATTTAGCGCTCAATATGCGCGCCATTGTTGCCCAGCA
ATTGGTGCCCACCGCCGATGGTAAAGGGCGGGTGGCGGCCATAGAAGTGCTACTCAACTCGCCACTGGTGAGTGACCTAA
TCGCTCGTAATGACATCGGTGAGCTTAAGGAGGTAATGCAAAAAAGCCAAGAAATGGGCATGCAAACCTTCGATATGGCG
CTGTATCAGCTGTACAAAGAACAGAAGATCACCAAAGATCAGGCGTTGCACCATGCAGACTCGCCCAATGACTTGCGTCT
GATGATTAAGCTGCAAAGTGATGATGGGCCGGATTTAGGCTCGCTTTCCGATGTCTCCATTGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2E5G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.242

98.378

0.573
  pilU Vibrio cholerae strain A1552

57.297

100

0.573
  pilU Acinetobacter baylyi ADP1

58.358

92.162

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.417

96.486

0.419
  pilT Legionella pneumophila strain Lp02

40.597

90.541

0.368
  pilT Legionella pneumophila strain ERS1305867

40.597

90.541

0.368

Multiple sequence alignment