Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DGN21_RS13605 Genome accession   NZ_CP029263
Coordinates   3231544..3232581 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas sp. MLO165     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3226544..3237581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DGN21_RS13585 (DGN21_13870) - 3226818..3228662 (+) 1845 WP_126965876.1 DUF4153 domain-containing protein -
  DGN21_RS13590 (DGN21_13875) - 3228659..3229204 (+) 546 WP_054591431.1 DNA-3-methyladenine glycosylase I -
  DGN21_RS13595 (DGN21_13880) - 3229341..3230015 (-) 675 WP_054591432.1 YitT family protein -
  DGN21_RS13600 (DGN21_13885) pilU 3230301..3231431 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DGN21_RS13605 (DGN21_13890) pilT 3231544..3232581 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  DGN21_RS13610 (DGN21_13895) - 3232904..3233596 (+) 693 WP_215824949.1 YggS family pyridoxal phosphate-dependent enzyme -
  DGN21_RS13615 (DGN21_13900) proC 3233658..3234503 (+) 846 WP_215824950.1 pyrroline-5-carboxylate reductase -
  DGN21_RS13620 (DGN21_13905) - 3235081..3235506 (+) 426 WP_026064288.1 HU family DNA-binding protein -
  DGN21_RS13625 (DGN21_13910) - 3235640..3236260 (+) 621 WP_160954510.1 M23 family metallopeptidase -
  DGN21_RS13630 (DGN21_13915) - 3236353..3236661 (+) 309 WP_024940752.1 DUF3861 family protein -
  DGN21_RS13635 (DGN21_13920) - 3236932..3237252 (+) 321 WP_080639692.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=291463 DGN21_RS13605 WP_003489919.1 3231544..3232581(-) (pilT) [Xanthomonas sp. MLO165]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=291463 DGN21_RS13605 WP_003489919.1 3231544..3232581(-) (pilT) [Xanthomonas sp. MLO165]
ATGGATATCGCTGAACTATTGGCGTTTTCCGTCAAAAACAAGGCATCGGACCTGCACCTGTCGGCCGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCCCTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCTTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGTTGATCGACCAGCCGCAGGGTTTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACGGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAAAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGATCCGGACATCATCCTGGTCGGCGAATTACGCGATCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGACGCACCGCAGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAGTACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCACGCGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment