Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DGN21_RS13600 Genome accession   NZ_CP029263
Coordinates   3230301..3231431 (-) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas sp. MLO165     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3225301..3236431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DGN21_RS13575 (DGN21_13860) ruvX 3225712..3226179 (-) 468 WP_024939388.1 Holliday junction resolvase RuvX -
  DGN21_RS13580 (DGN21_13865) - 3226172..3226738 (-) 567 WP_011051924.1 YqgE/AlgH family protein -
  DGN21_RS13585 (DGN21_13870) - 3226818..3228662 (+) 1845 WP_126965876.1 DUF4153 domain-containing protein -
  DGN21_RS13590 (DGN21_13875) - 3228659..3229204 (+) 546 WP_054591431.1 DNA-3-methyladenine glycosylase I -
  DGN21_RS13595 (DGN21_13880) - 3229341..3230015 (-) 675 WP_054591432.1 YitT family protein -
  DGN21_RS13600 (DGN21_13885) pilU 3230301..3231431 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DGN21_RS13605 (DGN21_13890) pilT 3231544..3232581 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  DGN21_RS13610 (DGN21_13895) - 3232904..3233596 (+) 693 WP_215824949.1 YggS family pyridoxal phosphate-dependent enzyme -
  DGN21_RS13615 (DGN21_13900) proC 3233658..3234503 (+) 846 WP_215824950.1 pyrroline-5-carboxylate reductase -
  DGN21_RS13620 (DGN21_13905) - 3235081..3235506 (+) 426 WP_026064288.1 HU family DNA-binding protein -
  DGN21_RS13625 (DGN21_13910) - 3235640..3236260 (+) 621 WP_160954510.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=291462 DGN21_RS13600 WP_016902355.1 3230301..3231431(-) (pilU) [Xanthomonas sp. MLO165]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=291462 DGN21_RS13600 WP_016902355.1 3230301..3231431(-) (pilU) [Xanthomonas sp. MLO165]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGTCCGATCACCCAGACCCCGCTGACCGCGCAACAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATTGGCGTGTCCGGG
GTCGGGCGCTTTCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACGGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCATGCCGTGGCCTTCGCCGAAACCGGCCATCTGGTGTTGTGCACCCTGCACGCCAACAACGCAAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGTTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGTTGATTCCGACCCCGGATGGGCGCGGCCGCCGCGTGGCCATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGTCGCAGGGTGGCGACGCCAAGACCCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCCG
AGGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment