Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DEH84_RS15260 Genome accession   NZ_CP029210
Coordinates   3380782..3381825 (-) Length   347 a.a.
NCBI ID   WP_109037620.1    Uniprot ID   A0A2U8FU88
Organism   Aquabacterium olei strain NBRC 110486     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3375782..3386825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEH84_RS15230 (DEH84_15230) - 3376311..3376691 (+) 381 WP_109037614.1 YraN family protein -
  DEH84_RS15235 (DEH84_15235) - 3376736..3377317 (+) 582 WP_159098989.1 SIS domain-containing protein -
  DEH84_RS15240 (DEH84_15240) - 3377314..3377982 (+) 669 WP_109037616.1 BON domain-containing protein -
  DEH84_RS15245 (DEH84_15245) - 3378022..3378924 (-) 903 WP_109037617.1 NAD(P)-dependent oxidoreductase -
  DEH84_RS15250 (DEH84_15250) pilU 3378938..3380074 (-) 1137 WP_109037618.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DEH84_RS15255 (DEH84_15255) - 3380096..3380773 (-) 678 WP_109037619.1 Crp/Fnr family transcriptional regulator -
  DEH84_RS15260 (DEH84_15260) pilT 3380782..3381825 (-) 1044 WP_109037620.1 type IV pilus twitching motility protein PilT Machinery gene
  DEH84_RS15265 (DEH84_15265) - 3381863..3382549 (+) 687 WP_109037621.1 YggS family pyridoxal phosphate-dependent enzyme -
  DEH84_RS15270 (DEH84_15270) - 3382587..3383306 (+) 720 WP_109037622.1 response regulator transcription factor -
  DEH84_RS15275 (DEH84_15275) - 3383303..3385177 (+) 1875 WP_109037623.1 ATP-binding protein -
  DEH84_RS15280 (DEH84_15280) proC 3385186..3386007 (+) 822 WP_109037624.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38207.85 Da        Isoelectric Point: 7.0669

>NTDB_id=291246 DEH84_RS15260 WP_109037620.1 3380782..3381825(-) (pilT) [Aquabacterium olei strain NBRC 110486]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKQVHDMVYDIMNDAQRKTYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNAPKVFADLALRPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPHTMSFANALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGNSMGMQTLDQN
LTELVRRNVISPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=291246 DEH84_RS15260 WP_109037620.1 3380782..3381825(-) (pilT) [Aquabacterium olei strain NBRC 110486]
ATGGATATCACCCAGCTCCTTGCGTTCTCCGTCAAGAACAAGGCATCCGACCTTCACCTGTCGGCAGGGTTGCCCCCCAT
GATCCGGGTCCACGGCGATGTGCGCCGCATCAACGTCGACGCGCTGGACCACAAGCAGGTCCACGACATGGTGTACGACA
TCATGAACGACGCCCAGCGCAAGACCTATGAAGAAACGCTGGAGTGCGACTTCTCGTTCGAGATCCAGGGGCTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGTGCCGGCGCCGTGTTCCGGACGATTCCGTCCAAGATCCTGACGCTCGA
GCAGCTCAACGCACCCAAGGTGTTTGCCGACCTGGCCTTGAGGCCGCGCGGGCTGGTGCTCGTCACGGGGCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGAACCACCTCAACGAGACCGAGTACGGCCACATCCTGACGGTCGAGGAC
CCGATCGAGTTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGCACACCATGAGCTTTGCCAA
CGCGCTGCGCTCGGCCCTGCGCGAGGACCCGGATGCGATTCTGGTGGGTGAAATGCGCGACCTGGAAACCATCCGCCTCG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGCGTG
GTCGATGTCTTCCCCGCCGCCGAGAAGGAAATGGTGCGCGCCATGCTGTCCGAGTCGCTGCAGGCGGTGATCTCCCAGAC
GCTGTGCAAGACGAAGGACGGCTCGGGCCGGGTGGCCGCGCACGAAATCATGATCGGCACCCCCGCGATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGTCGATCCAGACCGGCAACAGCATGGGCATGCAGACGCTGGATCAGAAC
CTGACCGAACTGGTGCGACGCAACGTCATCTCGCCCGCCGAGGCCCGCGCCAAGGCCAAGATTCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8FU88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Pseudomonas stutzeri DSM 10701

77.681

99.424

0.772
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain ERS1305867

72.271

97.695

0.706
  pilT Legionella pneumophila strain Lp02

72.271

97.695

0.706
  pilT Neisseria meningitidis 8013

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment