Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DEH84_RS15250 Genome accession   NZ_CP029210
Coordinates   3378938..3380074 (-) Length   378 a.a.
NCBI ID   WP_109037618.1    Uniprot ID   -
Organism   Aquabacterium olei strain NBRC 110486     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3373938..3385074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEH84_RS15220 (DEH84_15220) - 3374284..3375234 (-) 951 WP_109038428.1 triacylglycerol lipase -
  DEH84_RS15225 (DEH84_15225) rsmI 3375407..3376312 (-) 906 WP_109037613.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  DEH84_RS15230 (DEH84_15230) - 3376311..3376691 (+) 381 WP_109037614.1 YraN family protein -
  DEH84_RS15235 (DEH84_15235) - 3376736..3377317 (+) 582 WP_159098989.1 SIS domain-containing protein -
  DEH84_RS15240 (DEH84_15240) - 3377314..3377982 (+) 669 WP_109037616.1 BON domain-containing protein -
  DEH84_RS15245 (DEH84_15245) - 3378022..3378924 (-) 903 WP_109037617.1 NAD(P)-dependent oxidoreductase -
  DEH84_RS15250 (DEH84_15250) pilU 3378938..3380074 (-) 1137 WP_109037618.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DEH84_RS15255 (DEH84_15255) - 3380096..3380773 (-) 678 WP_109037619.1 Crp/Fnr family transcriptional regulator -
  DEH84_RS15260 (DEH84_15260) pilT 3380782..3381825 (-) 1044 WP_109037620.1 type IV pilus twitching motility protein PilT Machinery gene
  DEH84_RS15265 (DEH84_15265) - 3381863..3382549 (+) 687 WP_109037621.1 YggS family pyridoxal phosphate-dependent enzyme -
  DEH84_RS15270 (DEH84_15270) - 3382587..3383306 (+) 720 WP_109037622.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41940.26 Da        Isoelectric Point: 6.8328

>NTDB_id=291245 DEH84_RS15250 WP_109037618.1 3378938..3380074(-) (pilU) [Aquabacterium olei strain NBRC 110486]
MERDQASKFINDLLKLMVARGGSDLFLTSDFPPAIKVDGKVTKVSPQALTSQHTVSLARAIMNDKQAAEFERTKECNFAI
SPGGIGRFRVNAFIQQGNVGLVLRTIPQNLPTVDGMGLPQILKEVVLTKRGLVILVGGTGSGKSTTLAAMVDHRNENTHG
HIITLEDPVEFVHPHKNCIITQREIGIDTDGWEMALKNTLRQAPDVILMGEIRDRETMEHAVQFAETGHLCMATLHANSA
NQALDRIINFFPEERRPQLLMDLSLNLKALVSQRLLPRQEGKGRVAAVEILLNTPLIGDLIFKGEVGEIKEIMKKSRELG
MQTFDQALYDLYESNYVTYEDALRNADSVNDLRLQIKLNSTRARTSDLAAGTEHLKIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=291245 DEH84_RS15250 WP_109037618.1 3378938..3380074(-) (pilU) [Aquabacterium olei strain NBRC 110486]
ATGGAACGCGATCAGGCCTCCAAATTCATCAACGACCTGCTGAAGCTCATGGTCGCCCGGGGGGGCTCCGACCTGTTCCT
CACGTCGGACTTCCCGCCCGCCATCAAGGTCGACGGCAAGGTCACCAAGGTCTCGCCGCAGGCCCTCACGTCGCAGCACA
CCGTGTCGCTGGCCCGCGCGATCATGAACGACAAGCAGGCGGCCGAGTTCGAGCGCACAAAGGAGTGCAACTTCGCGATC
TCGCCCGGCGGCATCGGCCGCTTCCGCGTCAATGCCTTCATCCAGCAGGGCAATGTGGGCCTGGTGCTGCGGACCATCCC
GCAGAACCTGCCCACCGTCGACGGCATGGGCCTGCCCCAGATCCTCAAGGAGGTCGTGCTGACCAAGCGCGGACTGGTGA
TCCTGGTGGGTGGCACGGGCTCTGGCAAGTCGACCACGCTGGCGGCCATGGTCGACCATCGCAACGAGAACACGCACGGC
CACATCATCACGCTGGAAGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCATCATCACCCAGCGTGAGATCGGCAT
CGACACCGACGGCTGGGAGATGGCGCTGAAGAACACCCTGCGCCAGGCGCCCGATGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGCAGTTTGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AATCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGGCCGCAGCTGCTGATGGACCTGTCGCTCAACCT
GAAGGCACTGGTCTCGCAACGCCTGCTGCCGCGTCAGGAAGGCAAGGGCCGCGTGGCCGCGGTCGAGATCCTGCTCAACA
CGCCGCTGATCGGCGACCTCATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTACGACCTCTACGAGAGCAACTACGTGACCTACGAAGATGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCACCCGCGCGCGCACCAGCGACCTGGCCGCCGGCACCGAAC
ACCTGAAGATTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.218

92.063

0.582
  pilU Acinetobacter baylyi ADP1

56.98

92.857

0.529
  pilU Vibrio cholerae strain A1552

52.342

96.032

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.588

89.153

0.415
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

44.72

85.185

0.381
  pilT Legionella pneumophila strain Lp02

42.342

88.095

0.373
  pilT Legionella pneumophila strain ERS1305867

42.342

88.095

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.164

83.862

0.37
  pilT Vibrio cholerae strain A1552

44.164

83.862

0.37

Multiple sequence alignment