Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VIBHAR_RS16750 Genome accession   NC_009783
Coordinates   3625644..3626684 (-) Length   346 a.a.
NCBI ID   WP_012128942.1    Uniprot ID   A7MTP9
Organism   Vibrio campbellii ATCC BAA-1116     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3620644..3631684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBHAR_RS16720 (VIBHAR_03571) endA 3620740..3621435 (+) 696 WP_005425828.1 deoxyribonuclease I -
  VIBHAR_RS16725 (VIBHAR_03572) rsmE 3621582..3622313 (+) 732 WP_005425825.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VIBHAR_RS16730 (VIBHAR_03573) gshB 3622328..3623278 (+) 951 WP_005425823.1 glutathione synthase -
  VIBHAR_RS16735 (VIBHAR_03574) - 3623408..3623971 (+) 564 WP_038892068.1 YqgE/AlgH family protein -
  VIBHAR_RS16740 (VIBHAR_03575) ruvX 3624019..3624444 (+) 426 WP_021017977.1 Holliday junction resolvase RuvX -
  VIBHAR_RS16745 (VIBHAR_03576) pilU 3624506..3625612 (-) 1107 WP_005425812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VIBHAR_RS16750 (VIBHAR_03577) pilT 3625644..3626684 (-) 1041 WP_012128942.1 type IV pilus twitching motility protein PilT Machinery gene
  VIBHAR_RS16755 (VIBHAR_03578) - 3626713..3627423 (+) 711 WP_012128943.1 YggS family pyridoxal phosphate-dependent enzyme -
  VIBHAR_RS16760 (VIBHAR_03579) proC 3627554..3628372 (+) 819 WP_012128944.1 pyrroline-5-carboxylate reductase -
  VIBHAR_RS16765 (VIBHAR_03580) - 3628426..3628983 (+) 558 WP_005425806.1 YggT family protein -
  VIBHAR_RS16770 (VIBHAR_03581) yggU 3628983..3629273 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  VIBHAR_RS16775 (VIBHAR_03582) - 3629333..3629764 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  VIBHAR_RS16780 (VIBHAR_03583) - 3629870..3630472 (+) 603 WP_012128945.1 XTP/dITP diphosphatase -
  VIBHAR_RS16785 (VIBHAR_03584) hemW 3630491..3631663 (+) 1173 WP_009705540.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38650.12 Da        Isoelectric Point: 6.5043

>NTDB_id=29044 VIBHAR_RS16750 WP_012128942.1 3625644..3626684(-) (pilT) [Vibrio campbellii ATCC BAA-1116]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANSEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=29044 VIBHAR_RS16750 WP_012128942.1 3625644..3626684(-) (pilT) [Vibrio campbellii ATCC BAA-1116]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTAAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGACTTTTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCGGTGTTCCGTACCATCCCAAGTAGCATTCCAACACTGGA
AGAGCTAGAAGCTCCTGAGATCTTCAGAAAAATCGCCAATTCGGAAAAAGGTTTGGTACTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACTACCTTGGCTGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACGTCCTAACAATTGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCGCTACGCAGCGCGCTGCGTGAAGACCCGGATGTGATCCTAGTTGGTGAGATGCGTGATAAAGAAACTATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGTTGCACACTAGCTCGGCAGCGAAAACCATCGACCGTATT
ATTGACGTCTTCCCTGGTAGCGACAAAGACATGGTGCGTTCGATGCTGTCTGAATCATTGCGTTCGGTTATCGCGCAAAA
ACTTCTGAAACGTAACGGCGGCGGTCGAATCGCGTGTCACGAAATCATGATGGCTACGCCTGCGATCCGTAACCTTATCC
GTGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGTGCGGCGCATGGTATGCAGACCATGGAGCAGAATGCT
CGTCAATTGATGGCGCAAGGCATGGTATCGCGTGAAGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7MTP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.377

99.711

0.861
  pilT Vibrio cholerae strain A1552

86.377

99.711

0.861
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

68.824

98.266

0.676
  pilT Pseudomonas stutzeri DSM 10701

69.027

97.977

0.676
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

64.412

98.266

0.633
  pilT Neisseria gonorrhoeae MS11

64.118

98.266

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.57

100

0.5
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361

Multiple sequence alignment