Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BTJ40_RS01140 Genome accession   NZ_CP029064
Coordinates   232881..233915 (-) Length   344 a.a.
NCBI ID   WP_108731395.1    Uniprot ID   -
Organism   Microbulbifer sp. A4B17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 227881..238915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTJ40_RS01115 (BTJ40_01115) - 229152..230309 (+) 1158 WP_108731391.1 sensor histidine kinase -
  BTJ40_RS01120 (BTJ40_01120) - 230306..231052 (+) 747 WP_108731392.1 LytTR family DNA-binding domain-containing protein -
  BTJ40_RS01130 (BTJ40_01130) - 231124..231432 (-) 309 WP_108731393.1 SDR family NAD(P)-dependent oxidoreductase -
  BTJ40_RS01135 (BTJ40_01135) pilU 231669..232820 (-) 1152 WP_108731394.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BTJ40_RS01140 (BTJ40_01140) pilT 232881..233915 (-) 1035 WP_108731395.1 type IV pilus twitching motility protein PilT Machinery gene
  BTJ40_RS01145 (BTJ40_01145) - 233985..234689 (+) 705 WP_108731396.1 YggS family pyridoxal phosphate-dependent enzyme -
  BTJ40_RS01150 (BTJ40_01150) - 234727..235014 (+) 288 WP_157953851.1 hypothetical protein -
  BTJ40_RS01155 (BTJ40_01155) proC 235335..236210 (+) 876 WP_369974266.1 pyrroline-5-carboxylate reductase -
  BTJ40_RS01160 (BTJ40_01160) - 236276..236884 (+) 609 WP_108731398.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38345.04 Da        Isoelectric Point: 6.5788

>NTDB_id=289783 BTJ40_RS01140 WP_108731395.1 232881..233915(-) (pilT) [Microbulbifer sp. A4B17]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPSMEHKQVHGLIYEIMNDKQRKDYEELLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVISQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
EDLVARRIISREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=289783 BTJ40_RS01140 WP_108731395.1 232881..233915(-) (pilT) [Microbulbifer sp. A4B17]
ATGGATATCACTGAACTCCTCGCCTTTAGCGCCAAGCAAAACGCCTCGGACCTACACCTCTCGGCGGGCCTGCCGCCGAT
GATTCGTGTCGACGGCGACGTGCGTCGTATCAACCTGCCCTCGATGGAGCACAAGCAGGTACACGGCCTGATCTACGAGA
TCATGAACGATAAGCAGCGCAAGGATTACGAAGAACTACTGGAAACAGACTTCTCTTTTGAAGTCCCCGGTGTCGCCCGC
TTTCGTGTAAACGCCTTTAACCACAACCGGGGTGCCGGCGCCGTATTCCGGACCATTCCCTCCAAGGTTCTCACTATGGA
AGACCTGGGAATGGGCCAGGTTTTCAAGCAGATTTCAGATACTCCCCGCGGCTTGGTGCTGGTAACCGGGCCCACGGGTT
CCGGTAAGTCCACTACCCTGGCGGCGATGATGGACTACATCAACGACAATAAATACGAGCATATCCTCACCATTGAGGAC
CCGATCGAATTTGTGCACGAATCCAAGAAGTGCCTGGTGAACCAGCGTGAGGTACACCGGGATACCCACGGCTTTGCCGA
GGCACTGCGCTCTGCACTGCGTGAAGACCCGGACATTATTCTCGTGGGTGAGATGCGGGACCTGGAGACCATTCGCCTGG
CGCTGACTGCGGCAGAGACCGGTCACCTGGTATTCGGTACCCTGCACACCACTTCCGCCGCCAAAACCATTGACCGTGTG
GTGGATGTATTCCCGGCGCAGGAGAAAGCCATGGTGCGTTCAATGCTGTCGGAATCCCTACAGGCCGTTATCTCCCAGAC
GCTAATGAAGAAAAACGGCGGTGGCCGGGTCGCTGCCCACGAAATTATGCGTGGTACCCCAGCCATCCGTAACCTGATCC
GCGAAGACAAAATCGCGCAGATGTACTCGGCCATCCAGACCGGAGCCAGCGTCGGCATGCAGACCATGGACCAATGTCTG
GAAGACCTGGTGGCACGCCGCATTATCAGCCGCGAAGTGGCTCGCGAGAAAGCCAAGATGCCGGAGAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.244

100

0.892
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.547

0.404
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment