Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BTJ40_RS01135 Genome accession   NZ_CP029064
Coordinates   231669..232820 (-) Length   383 a.a.
NCBI ID   WP_108731394.1    Uniprot ID   -
Organism   Microbulbifer sp. A4B17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 226669..237820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTJ40_RS01100 (BTJ40_01100) - 226857..227207 (-) 351 WP_108731388.1 lipoprotein -
  BTJ40_RS01105 (BTJ40_01105) cyaY 227315..227641 (+) 327 WP_108731389.1 iron donor protein CyaY -
  BTJ40_RS01110 (BTJ40_01110) argH 227638..229062 (-) 1425 WP_108731390.1 argininosuccinate lyase -
  BTJ40_RS01115 (BTJ40_01115) - 229152..230309 (+) 1158 WP_108731391.1 sensor histidine kinase -
  BTJ40_RS01120 (BTJ40_01120) - 230306..231052 (+) 747 WP_108731392.1 LytTR family DNA-binding domain-containing protein -
  BTJ40_RS01130 (BTJ40_01130) - 231124..231432 (-) 309 WP_108731393.1 SDR family NAD(P)-dependent oxidoreductase -
  BTJ40_RS01135 (BTJ40_01135) pilU 231669..232820 (-) 1152 WP_108731394.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BTJ40_RS01140 (BTJ40_01140) pilT 232881..233915 (-) 1035 WP_108731395.1 type IV pilus twitching motility protein PilT Machinery gene
  BTJ40_RS01145 (BTJ40_01145) - 233985..234689 (+) 705 WP_108731396.1 YggS family pyridoxal phosphate-dependent enzyme -
  BTJ40_RS01150 (BTJ40_01150) - 234727..235014 (+) 288 WP_157953851.1 hypothetical protein -
  BTJ40_RS01155 (BTJ40_01155) proC 235335..236210 (+) 876 WP_369974266.1 pyrroline-5-carboxylate reductase -
  BTJ40_RS01160 (BTJ40_01160) - 236276..236884 (+) 609 WP_108731398.1 YggT family protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42498.72 Da        Isoelectric Point: 5.9763

>NTDB_id=289782 BTJ40_RS01135 WP_108731394.1 231669..232820(-) (pilU) [Microbulbifer sp. A4B17]
MDIERLLQLVVEKGASDLFITAGVPPSIKLHGRVVPCSSTALTPEKARELVVGTMNEKQRREFAEQKELNFAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPDVLKDLAMTKRGLIIFVGATGTGKSTSLASMIGHRNENTKGHIITIED
PIEFIHQHRGCIVTQREVGLDTESFDVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHKQLFMDLSLNLKAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLIRKGEVDKIKELMKRSGEQGMQTFDQA
LYELYDGGEITYEDALSHADSANDLRLMIKLKSESDAEYLNSAAGELSLQNDSEYGDGGPQLY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=289782 BTJ40_RS01135 WP_108731394.1 231669..232820(-) (pilU) [Microbulbifer sp. A4B17]
ATGGATATTGAAAGACTTCTACAACTGGTTGTAGAGAAGGGCGCTTCGGATTTATTTATCACGGCGGGCGTCCCGCCCTC
CATCAAACTGCACGGCCGAGTGGTGCCCTGCAGCAGCACCGCGCTCACGCCAGAGAAGGCGCGCGAGCTGGTGGTGGGAA
CCATGAACGAGAAACAGCGGCGCGAGTTTGCCGAGCAAAAGGAATTGAACTTCGCGATTGCAGTGCGCAACGTGGGGCGT
TTCCGGGTATCGGCCTTCTTCCAGCGCAATCTGGTGGGTATGGTGCTGCGTCGCATCGAAACCAAAATCCCCACTATCGA
TGGACTCGGACTACCCGATGTACTCAAAGACCTGGCCATGACCAAACGCGGGCTGATTATTTTTGTCGGCGCCACCGGTA
CCGGTAAGTCCACATCCCTCGCCTCCATGATCGGCCACCGCAATGAGAACACCAAGGGCCATATCATCACCATTGAGGAC
CCGATCGAATTTATCCACCAGCATCGCGGCTGTATTGTGACCCAGCGCGAAGTGGGCCTGGATACCGAGTCTTTCGATGT
AGCCCTGAAAAACACTCTGCGTCAGGCGCCAGATGTTATTCTGATCGGTGAGGTACGCTCCCGCGAAACCATGGACCACG
CCATCGCTTTCGCCGAGACCGGCCATTTGTGTCTGTGCACCCTCCACGCCAACAATGCCAACCAGGCCCTCGACCGGATC
ATTCACTTTTTCCCGGCAGACCGCCACAAACAGTTGTTTATGGACCTGTCCCTCAACCTGAAGGCGATGGTGGCCCAGCA
GCTGGTACCGACCCCCGATGGCGATGGCCGCCGTGCCTGCCTGGAGATCATGATCAATACCCCACTGATGTCGGACCTGA
TCCGCAAGGGCGAAGTTGATAAGATCAAGGAGTTGATGAAGCGCTCTGGTGAGCAGGGAATGCAAACCTTCGATCAGGCA
CTCTACGAGCTCTACGACGGGGGAGAAATTACCTATGAGGATGCCCTCTCCCACGCCGACTCTGCCAACGATTTGCGCCT
GATGATTAAGCTCAAATCCGAATCCGATGCCGAGTACTTAAATAGTGCCGCTGGTGAGTTGAGTCTACAAAATGATTCAG
AGTACGGAGACGGTGGTCCTCAGCTCTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.135

96.606

0.726
  pilU Acinetobacter baylyi ADP1

67.787

93.211

0.632
  pilU Vibrio cholerae strain A1552

54.987

96.867

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.313

88.512

0.41
  pilT Acinetobacter nosocomialis M2

43.068

88.512

0.381
  pilT Acinetobacter baumannii D1279779

43.068

88.512

0.381
  pilT Acinetobacter baumannii strain A118

43.068

88.512

0.381
  pilT Pseudomonas aeruginosa PAK

41.888

88.512

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.84

87.99

0.368
  pilT Vibrio cholerae strain A1552

41.84

87.99

0.368
  pilT Acinetobacter baylyi ADP1

41.056

89.034

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.298

88.512

0.366
  pilT Legionella pneumophila strain Lp02

41.791

87.467

0.366
  pilT Legionella pneumophila strain ERS1305867

41.791

87.467

0.366

Multiple sequence alignment