Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DBO93_RS05320 Genome accession   NZ_CP028924
Coordinates   1209104..1210147 (+) Length   347 a.a.
NCBI ID   WP_108455394.1    Uniprot ID   A0A7W2YMX3
Organism   Colwellia sp. Arc7-D     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1204104..1215147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBO93_RS05290 (DBO93_05290) - 1204476..1205078 (-) 603 WP_204100650.1 XTP/dITP diphosphatase -
  DBO93_RS05295 (DBO93_05295) - 1205254..1206081 (+) 828 WP_239059123.1 EamA family transporter -
  DBO93_RS05300 (DBO93_05300) - 1206258..1206698 (-) 441 WP_108455390.1 DUF4426 domain-containing protein -
  DBO93_RS05305 (DBO93_05305) - 1206770..1207318 (-) 549 WP_108455391.1 YggT family protein -
  DBO93_RS05310 (DBO93_05310) proC 1207411..1208223 (-) 813 WP_108455392.1 pyrroline-5-carboxylate reductase -
  DBO93_RS05315 (DBO93_05315) - 1208360..1209034 (-) 675 WP_108455393.1 YggS family pyridoxal phosphate-dependent enzyme -
  DBO93_RS05320 (DBO93_05320) pilT 1209104..1210147 (+) 1044 WP_108455394.1 type IV pilus twitching motility protein PilT Machinery gene
  DBO93_RS05325 (DBO93_05325) pilU 1210173..1211300 (+) 1128 WP_108455395.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DBO93_RS05330 (DBO93_05330) - 1211643..1211915 (+) 273 WP_162533730.1 EAL domain-containing protein -
  DBO93_RS05335 (DBO93_05335) - 1212066..1212611 (+) 546 WP_108455397.1 DsrE family protein -
  DBO93_RS05340 (DBO93_05340) - 1212628..1213560 (-) 933 WP_108455398.1 AEC family transporter -
  DBO93_RS05345 (DBO93_05345) ruvX 1213648..1214085 (-) 438 WP_108455399.1 Holliday junction resolvase RuvX -
  DBO93_RS05350 (DBO93_05350) - 1214085..1214639 (-) 555 WP_108455400.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38687.22 Da        Isoelectric Point: 5.9618

>NTDB_id=288585 DBO93_RS05320 WP_108455394.1 1209104..1210147(+) (pilT) [Colwellia sp. Arc7-D]
MDITELLAFSVENNASDLHLSTGTPPSIRVDGDVRKLNIPAFDEKDVNALVYDIMNDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDDLGCPDIFRDISMLPRGLVLVTGPTGSGKSTTLAAMVDHINKTKHDHILTIED
PIEFVHQNHSCLINQREVHRDTLSFSAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLVKKVGGGRVASHEIMMGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QNLINRGMITRQDAMDKAQDKNQFTGY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=288585 DBO93_RS05320 WP_108455394.1 1209104..1210147(+) (pilT) [Colwellia sp. Arc7-D]
ATGGATATTACCGAATTACTCGCATTTAGTGTTGAAAATAATGCTTCAGATTTACATTTATCAACCGGCACACCCCCGTC
AATTCGCGTTGATGGTGACGTACGTAAGCTAAATATACCAGCATTTGACGAAAAAGATGTCAACGCTTTGGTCTATGATA
TTATGAATGATCGCCAACGCAAAGAATATGAAGAAAATTTAGAGGTCGATTTCTCTTTTGAAGTGCCAAATTTAGCACGT
TTCAGGGTAAATGCATTTAATCAAAACCGTGGCCCTGCAGCCGTATTTCGTACCATACCAAGTAAAATTTTATCATTAGA
CGATTTAGGTTGCCCTGATATTTTTAGAGATATATCTATGCTTCCTCGTGGGTTAGTGCTTGTTACTGGACCAACGGGTT
CAGGTAAGTCAACCACACTCGCGGCTATGGTTGATCATATAAATAAAACCAAACATGATCACATTTTAACCATTGAAGAT
CCTATCGAGTTTGTTCATCAAAACCATTCATGTTTGATTAACCAACGTGAAGTTCATCGCGATACCTTAAGTTTTAGCGC
GGCATTACGCTCAGCATTACGTGAAGATCCCGATGTAATTTTAGTTGGTGAAATGCGTGATTTAGAAACCATACGTTTAG
CGATGACCGCAGCTGAAACCGGCCATTTAGTGTTTGGCACCTTACATACAACTTCTGCACCTAAAACCATTGACCGTATT
ATTGATGTGTTTCCGGGTGAAGAAAAAGCTATGGTGCGTTCAATGCTTTCTGAATCACTACGCGCGGTAATCTCACAAAC
GCTAGTTAAAAAAGTGGGTGGCGGTCGTGTTGCTTCACACGAAATAATGATGGGTGTACCGGCAATTCGTAACCTTATTC
GTGAAGATAAAATTGCGCAAATGTATTCAGCGATTCAAACCGGTATGTCTCATGGCATGCAAACAATGGATCAATGTTTA
CAAAACCTGATCAACCGCGGCATGATCACCAGACAAGATGCCATGGATAAAGCACAAGACAAAAACCAATTTACTGGTTA
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7W2YMX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.872

99.135

0.752
  pilT Acinetobacter nosocomialis M2

75.581

99.135

0.749
  pilT Acinetobacter baumannii D1279779

75.581

99.135

0.749
  pilT Acinetobacter baumannii strain A118

75.581

99.135

0.749
  pilT Pseudomonas aeruginosa PAK

74.709

99.135

0.741
  pilT Acinetobacter baylyi ADP1

74.709

99.135

0.741
  pilT Legionella pneumophila strain Lp02

72.384

99.135

0.718
  pilT Legionella pneumophila strain ERS1305867

72.384

99.135

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.695

0.697
  pilT Vibrio cholerae strain A1552

71.386

97.695

0.697
  pilT Neisseria meningitidis 8013

67.052

99.712

0.669
  pilT Neisseria gonorrhoeae MS11

66.763

99.712

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.424

0.522
  pilU Pseudomonas stutzeri DSM 10701

41.399

98.847

0.409
  pilU Vibrio cholerae strain A1552

40.525

98.847

0.401
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395

Multiple sequence alignment