Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DBO93_RS05325 Genome accession   NZ_CP028924
Coordinates   1210173..1211300 (+) Length   375 a.a.
NCBI ID   WP_108455395.1    Uniprot ID   A0A7W2YMF8
Organism   Colwellia sp. Arc7-D     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1205173..1216300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBO93_RS05295 (DBO93_05295) - 1205254..1206081 (+) 828 WP_239059123.1 EamA family transporter -
  DBO93_RS05300 (DBO93_05300) - 1206258..1206698 (-) 441 WP_108455390.1 DUF4426 domain-containing protein -
  DBO93_RS05305 (DBO93_05305) - 1206770..1207318 (-) 549 WP_108455391.1 YggT family protein -
  DBO93_RS05310 (DBO93_05310) proC 1207411..1208223 (-) 813 WP_108455392.1 pyrroline-5-carboxylate reductase -
  DBO93_RS05315 (DBO93_05315) - 1208360..1209034 (-) 675 WP_108455393.1 YggS family pyridoxal phosphate-dependent enzyme -
  DBO93_RS05320 (DBO93_05320) pilT 1209104..1210147 (+) 1044 WP_108455394.1 type IV pilus twitching motility protein PilT Machinery gene
  DBO93_RS05325 (DBO93_05325) pilU 1210173..1211300 (+) 1128 WP_108455395.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DBO93_RS05330 (DBO93_05330) - 1211643..1211915 (+) 273 WP_162533730.1 EAL domain-containing protein -
  DBO93_RS05335 (DBO93_05335) - 1212066..1212611 (+) 546 WP_108455397.1 DsrE family protein -
  DBO93_RS05340 (DBO93_05340) - 1212628..1213560 (-) 933 WP_108455398.1 AEC family transporter -
  DBO93_RS05345 (DBO93_05345) ruvX 1213648..1214085 (-) 438 WP_108455399.1 Holliday junction resolvase RuvX -
  DBO93_RS05350 (DBO93_05350) - 1214085..1214639 (-) 555 WP_108455400.1 YqgE/AlgH family protein -
  DBO93_RS05355 (DBO93_05355) gshB 1214714..1215667 (-) 954 WP_108455401.1 glutathione synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41047.91 Da        Isoelectric Point: 5.7093

>NTDB_id=288586 DBO93_RS05325 WP_108455395.1 1210173..1211300(+) (pilU) [Colwellia sp. Arc7-D]
MSFHDLLQKMVQHGASDMFVTAKLAVSAKVNGELVPIDDHILSASESLGLVHDAMSEKQKTAFDEEKECNFAISIDDIGR
FRISAFWQRDMAGMVIRRIVTEIPDADELGLPSVLKDVVMSKRGLVLFVGGTGTGKSTSMAALIGYRNKNSRGHILTIED
PVEFVHEHRKCMVTQREVGLDTESFGAALKSSLRQAPDVILIGEIRNQETMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPAEQHGKLLFDLALNLRGIIAQQLIPTRDGNGRLAAIEVLLNSPYVAELIKKGDVGSIKEVMEKSTDQGMQTFDQA
LFNLYQQGLINYADALHHADSPNDLRLMIKLRSNDQGGTGSLSGVTIDGLAPKED

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=288586 DBO93_RS05325 WP_108455395.1 1210173..1211300(+) (pilU) [Colwellia sp. Arc7-D]
ATGAGTTTTCATGATTTATTACAAAAAATGGTGCAGCACGGCGCATCAGATATGTTTGTTACCGCTAAATTAGCTGTAAG
CGCAAAAGTTAATGGGGAACTTGTTCCTATTGACGATCATATTTTATCGGCCTCTGAATCTTTAGGTTTGGTTCATGACG
CCATGTCAGAAAAGCAAAAAACTGCATTTGATGAAGAAAAAGAATGTAACTTTGCAATTTCAATTGACGATATAGGACGA
TTTCGTATATCGGCGTTTTGGCAACGAGACATGGCTGGTATGGTTATTCGCCGCATTGTTACCGAAATTCCAGATGCCGA
TGAATTAGGCTTACCTTCAGTATTGAAAGACGTGGTTATGTCAAAGCGTGGTTTAGTGCTATTTGTGGGTGGTACGGGTA
CGGGTAAGTCTACTTCGATGGCTGCGTTAATTGGTTATCGAAATAAAAACTCTCGTGGTCATATTTTAACTATAGAAGAT
CCGGTTGAGTTTGTTCATGAACACCGAAAATGTATGGTTACACAACGTGAAGTTGGCCTTGATACAGAAAGCTTTGGCGC
GGCACTTAAAAGCTCTTTACGCCAAGCACCAGACGTTATTCTAATTGGTGAAATTCGTAATCAAGAAACCATGGAGCACG
CGTTAAGTTTCGCTGAAACGGGTCATCTATGTATTGCGACCTTGCATGCTAACAATGCAAACCAAGCAATTGATCGTATT
ATGCATTTAGTACCTGCTGAACAGCATGGTAAGTTATTGTTTGACCTTGCGTTGAACCTGCGCGGCATTATTGCCCAACA
GCTTATTCCAACCCGCGATGGTAATGGTCGTTTAGCTGCTATTGAAGTGTTATTAAACTCTCCATATGTTGCCGAATTAA
TTAAAAAAGGTGATGTGGGTAGTATTAAAGAAGTGATGGAAAAATCTACCGATCAAGGTATGCAAACATTTGACCAAGCA
CTCTTTAATTTATATCAACAAGGGCTAATTAACTACGCTGATGCACTGCATCATGCCGACTCACCAAACGATTTACGCTT
AATGATTAAATTACGCAGTAATGATCAAGGTGGAACCGGTTCATTGTCAGGTGTCACTATTGATGGTTTAGCGCCGAAAG
AAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7W2YMF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.185

95.2

0.592
  pilU Vibrio cholerae strain A1552

55.978

98.133

0.549
  pilU Acinetobacter baylyi ADP1

57.67

93.867

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.304

98.133

0.405
  pilT Pseudomonas aeruginosa PAK

40.29

92

0.371
  pilT Acinetobacter baylyi ADP1

40.351

91.2

0.368

Multiple sequence alignment