Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C8I07_RS01370 Genome accession   NZ_CP028355
Coordinates   327625..328662 (-) Length   345 a.a.
NCBI ID   WP_006082517.1    Uniprot ID   A0AA50Q7P7
Organism   Shewanella baltica strain CW2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322625..333662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C8I07_RS01335 (C8I07_01335) - 322734..323051 (+) 318 WP_006082510.1 hypothetical protein -
  C8I07_RS01340 (C8I07_01340) yciH 323230..323559 (+) 330 WP_037392612.1 stress response translation initiation inhibitor YciH -
  C8I07_RS01345 (C8I07_01345) - 323626..324189 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  C8I07_RS01350 (C8I07_01350) ruvX 324219..324644 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  C8I07_RS01355 (C8I07_01355) hemH 324730..325743 (-) 1014 WP_107402320.1 ferrochelatase -
  C8I07_RS01360 (C8I07_01360) - 325776..326315 (-) 540 WP_037392614.1 glutathione peroxidase -
  C8I07_RS01365 (C8I07_01365) pilU 326503..327615 (-) 1113 WP_107402321.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C8I07_RS01370 (C8I07_01370) pilT 327625..328662 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  C8I07_RS01375 (C8I07_01375) - 328698..329396 (+) 699 WP_107404843.1 YggS family pyridoxal phosphate-dependent enzyme -
  C8I07_RS01380 (C8I07_01380) proC 329612..330430 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  C8I07_RS01385 (C8I07_01385) - 330479..331027 (+) 549 WP_011847368.1 YggT family protein -
  C8I07_RS01390 (C8I07_01390) yggU 331027..331326 (+) 300 WP_107402322.1 DUF167 family protein YggU -
  C8I07_RS01395 (C8I07_01395) - 331457..331891 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -
  C8I07_RS01400 (C8I07_01400) rdgB 332031..332648 (+) 618 WP_107402323.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.94 Da        Isoelectric Point: 6.9095

>NTDB_id=284315 C8I07_RS01370 WP_006082517.1 327625..328662(-) (pilT) [Shewanella baltica strain CW2]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=284315 C8I07_RS01370 WP_006082517.1 327625..328662(-) (pilT) [Shewanella baltica strain CW2]
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAGATTAACCTGCCCGCGCTCGATCACCAAGGCGTACATAGCCTAGTGTACGACA
TAATGAATGATAAGCAGCGCAAGGACTACGAAGAACATTTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCGCGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCTGCGGCCGTATTTCGTACCATTCCTAGCGACATCTTGTCACTTGA
GCAATTAGGTGCGCCGGAGATTTTTAAAAAAATCGCCAGTTTCCCTCGGGGATTAGTCCTAGTGACTGGGCCAACAGGTT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTTAACGAAAATCGACACGATCACATCTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAAAACAAGCAATGTTTGATTAACCAGCGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTGCGTGAAGATCCGGACGTGATTTTAGTTGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGATGACCGCCGCAGAAACAGGCCACTTAGTGTTTGGTACCTTGCATACCACCTCCGCCGCGAAAACCATTGACCGTGTG
GTCGACGTATTCCCCGCCGGTGAAAAAGACATGGTACGCACTATGTTGTCGGAATCGCTGCAAGCGGTTATTTCGCAAAC
CCTGATTAAAAAGGTTGGTGGAGGCCGTGTCGCCGCCCATGAAATCATGATGGGCACTCCCGCTATCCGTAACCTTATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCGATTCAAACCGGTATGGCCCACGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACCCGTGAAGATGCAATGGCGAAGAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment