Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C8I07_RS01365 Genome accession   NZ_CP028355
Coordinates   326503..327615 (-) Length   370 a.a.
NCBI ID   WP_107402321.1    Uniprot ID   -
Organism   Shewanella baltica strain CW2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 321503..332615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C8I07_RS01330 (C8I07_01330) - 321995..322555 (-) 561 WP_006082509.1 DUF4136 domain-containing protein -
  C8I07_RS01335 (C8I07_01335) - 322734..323051 (+) 318 WP_006082510.1 hypothetical protein -
  C8I07_RS01340 (C8I07_01340) yciH 323230..323559 (+) 330 WP_037392612.1 stress response translation initiation inhibitor YciH -
  C8I07_RS01345 (C8I07_01345) - 323626..324189 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  C8I07_RS01350 (C8I07_01350) ruvX 324219..324644 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  C8I07_RS01355 (C8I07_01355) hemH 324730..325743 (-) 1014 WP_107402320.1 ferrochelatase -
  C8I07_RS01360 (C8I07_01360) - 325776..326315 (-) 540 WP_037392614.1 glutathione peroxidase -
  C8I07_RS01365 (C8I07_01365) pilU 326503..327615 (-) 1113 WP_107402321.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C8I07_RS01370 (C8I07_01370) pilT 327625..328662 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  C8I07_RS01375 (C8I07_01375) - 328698..329396 (+) 699 WP_107404843.1 YggS family pyridoxal phosphate-dependent enzyme -
  C8I07_RS01380 (C8I07_01380) proC 329612..330430 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  C8I07_RS01385 (C8I07_01385) - 330479..331027 (+) 549 WP_011847368.1 YggT family protein -
  C8I07_RS01390 (C8I07_01390) yggU 331027..331326 (+) 300 WP_107402322.1 DUF167 family protein YggU -
  C8I07_RS01395 (C8I07_01395) - 331457..331891 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41034.22 Da        Isoelectric Point: 6.3715

>NTDB_id=284314 C8I07_RS01365 WP_107402321.1 326503..327615(-) (pilU) [Shewanella baltica strain CW2]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDYLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=284314 C8I07_RS01365 WP_107402321.1 326503..327615(-) (pilU) [Shewanella baltica strain CW2]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTGTTTATCACAGCTGGCTTTCCGCCGAG
TGCCAAGATTGACGGTGAGTTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCACTGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCGACTCGTGAGTGTAACTTTGCTTTTGCGGCAAAGGATTTAGGTCGT
TTTCGGGTGAGTGCATTTTGGCAGCGTGAATCGCCAGGCTGCGTGATGCGCCGTATTGAGACTAAAATCCCTGACGTCGA
TTATTTAAAACTGCCCCCCATTCTGAAAGATCTGGTGATGAGTAAACGTGGTCTCATTATTATGGTGGGCGGAACGGGTA
CGGGTAAGTCGACATCCCTAGCGGCCTTGGTGGGGTATCGAAATGGTCATGCTCGTGGTCATATTCTCACGATTGAAGAT
CCGGTCGAATTTGTTCACGACCACAGAAAAAGCATTATCACCCAGCGTGAAGTGGGCATAGATACTGAGTCTTTCGATGC
AGCGCTAAAAAGCTCCCTTCGCCAAGCACCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CTCTGTCGTTTGCGGAAACAGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAGCAGTTATTGTTCGATTTGTCATTGAATTTACGCGGGATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGCACTGGGCGCCGCGCCGCGATTGAAGTATTGATTAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTACTCAAAGAAACCATGGCAAAATCCAACGAGCAGGGCATGCAGACGTTCGATCAAGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCGAACGATTTACGTTT
GATGATTAAATTACAGAATAAAGAATCCACGGGCTCAGGCTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.677

100

0.6
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378

Multiple sequence alignment