Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Tharo_RS02375 Genome accession   NZ_CP028339
Coordinates   488604..489647 (-) Length   347 a.a.
NCBI ID   WP_107219841.1    Uniprot ID   -
Organism   Thauera aromatica K172     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483604..494647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Tharo_RS02350 (Tharo_0468) fdh3B 484181..484807 (+) 627 WP_107219837.1 formate dehydrogenase FDH3 subunit beta -
  Tharo_RS02355 (Tharo_0469) - 484804..485040 (+) 237 WP_107219838.1 hypothetical protein -
  Tharo_RS02360 (Tharo_0470) - 485051..486067 (+) 1017 WP_245880981.1 formate dehydrogenase subunit gamma -
  Tharo_RS02365 (Tharo_0471) - 486246..487415 (+) 1170 WP_107219839.1 THUMP domain-containing protein -
  Tharo_RS02370 (Tharo_0472) pilU 487448..488581 (-) 1134 WP_107219840.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Tharo_RS02375 (Tharo_0473) pilT 488604..489647 (-) 1044 WP_107219841.1 type IV pilus twitching motility protein PilT Machinery gene
  Tharo_RS02380 (Tharo_0474) - 489762..490469 (+) 708 WP_107219842.1 YggS family pyridoxal phosphate-dependent enzyme -
  Tharo_RS02385 (Tharo_0475) proC 490516..491340 (+) 825 WP_107219843.1 pyrroline-5-carboxylate reductase -
  Tharo_RS02390 (Tharo_0476) - 491344..491925 (+) 582 WP_107219844.1 YggT family protein -
  Tharo_RS02395 (Tharo_0477) - 491932..492522 (-) 591 WP_107219845.1 Uma2 family endonuclease -
  Tharo_RS02400 (Tharo_0478) - 492616..493893 (-) 1278 WP_107219846.1 dihydroorotase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38489.09 Da        Isoelectric Point: 7.0222

>NTDB_id=284215 Tharo_RS02375 WP_107219841.1 488604..489647(-) (pilT) [Thauera aromatica K172]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKEVHDMVYDIMNDAQRKQFEESWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRTIPTKVLSLEELNSPKIFKDIASQPRGIVLVTGPTGSGKSTTLAAMVDYVNENNYGHILTVED
PIEFVHESKRCLINQREVHRDTMSFNNALRAALREDPDVVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGAGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNVGMQTLDQC
LADLVRRNIVSAAEARVKAQNKDNFAG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=284215 Tharo_RS02375 WP_107219841.1 488604..489647(-) (pilT) [Thauera aromatica K172]
ATGGACATCACCGAACTGCTCGCTTTCGCCGTCAAGAACAAGGCCTCCGACCTCCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCCATGGCGACGTGCGCCGCATCAACCTGCCGCCGATGGAGCACAAGGAAGTGCACGACATGGTGTATGACA
TCATGAACGACGCCCAGCGCAAGCAGTTCGAGGAGAGCTGGGAGTGCGACTTCTCCTTCGCCGTGCCCAACCTCGCCCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGGCGCGGTGTTCCGCACCATCCCGACGAAAGTGCTGTCCCTCGA
AGAGCTCAACAGCCCGAAGATCTTCAAGGACATCGCCAGCCAGCCGCGCGGCATCGTGCTCGTCACCGGGCCGACCGGCT
CGGGCAAATCGACTACGCTCGCGGCGATGGTCGACTACGTGAACGAAAACAACTACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCATGAATCGAAGCGCTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGATGTCGTTCAACAA
CGCCCTGCGCGCGGCGCTGCGCGAAGACCCCGACGTCGTCCTGGTGGGCGAGATGCGCGACCTCGAGACCATCCGCCTCG
CCCTCACCGCCGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACTTCGTCGGCGGCGAAGACCATCGACCGCATC
GTCGACGTCTTTCCCGCCGCCGAAAAGGACATGGTCCGCTCGATGCTTTCGGAATCGTTGCGGGCGGTAATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGTGCCGGCCGGGTCGCGGCGCACGAGATCATGATCGGCACTCCGGCGATCCGCAACCTGA
TCCGCGAAAACAAGATCGCCCAGATGTATTCGTCGATCCAGACCGGCCAGAACGTCGGCATGCAGACGCTGGACCAGTGC
CTGGCCGACCTTGTCCGGCGCAACATCGTGTCGGCGGCCGAGGCCCGGGTGAAGGCGCAGAACAAGGATAACTTCGCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.551

99.424

0.781
  pilT Acinetobacter baumannii D1279779

78.551

99.424

0.781
  pilT Acinetobacter baumannii strain A118

78.551

99.424

0.781
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Legionella pneumophila strain Lp02

73.333

99.424

0.729
  pilT Legionella pneumophila strain ERS1305867

73.333

99.424

0.729
  pilT Neisseria meningitidis 8013

70.52

99.712

0.703
  pilT Neisseria gonorrhoeae MS11

70.231

99.712

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

97.983

0.671
  pilT Vibrio cholerae strain A1552

68.529

97.983

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.601

99.712

0.524
  pilU Acinetobacter baylyi ADP1

41.954

100

0.421
  pilU Pseudomonas stutzeri DSM 10701

40.87

99.424

0.406
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406

Multiple sequence alignment