Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Tharo_RS02370 Genome accession   NZ_CP028339
Coordinates   487448..488581 (-) Length   377 a.a.
NCBI ID   WP_107219840.1    Uniprot ID   -
Organism   Thauera aromatica K172     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 482448..493581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Tharo_RS02350 (Tharo_0468) fdh3B 484181..484807 (+) 627 WP_107219837.1 formate dehydrogenase FDH3 subunit beta -
  Tharo_RS02355 (Tharo_0469) - 484804..485040 (+) 237 WP_107219838.1 hypothetical protein -
  Tharo_RS02360 (Tharo_0470) - 485051..486067 (+) 1017 WP_245880981.1 formate dehydrogenase subunit gamma -
  Tharo_RS02365 (Tharo_0471) - 486246..487415 (+) 1170 WP_107219839.1 THUMP domain-containing protein -
  Tharo_RS02370 (Tharo_0472) pilU 487448..488581 (-) 1134 WP_107219840.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Tharo_RS02375 (Tharo_0473) pilT 488604..489647 (-) 1044 WP_107219841.1 type IV pilus twitching motility protein PilT Machinery gene
  Tharo_RS02380 (Tharo_0474) - 489762..490469 (+) 708 WP_107219842.1 YggS family pyridoxal phosphate-dependent enzyme -
  Tharo_RS02385 (Tharo_0475) proC 490516..491340 (+) 825 WP_107219843.1 pyrroline-5-carboxylate reductase -
  Tharo_RS02390 (Tharo_0476) - 491344..491925 (+) 582 WP_107219844.1 YggT family protein -
  Tharo_RS02395 (Tharo_0477) - 491932..492522 (-) 591 WP_107219845.1 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42198.44 Da        Isoelectric Point: 6.6384

>NTDB_id=284214 Tharo_RS02370 WP_107219840.1 487448..488581(-) (pilU) [Thauera aromatica K172]
MERDQSLKFMHDLLRLMLQKNGSDLFITAGFPPAIKIDGRIVPQSNQLLQPQHTAELARAVMSDRQAAEFETHKECNFGI
SPAGIGRFRANAYIQQGKVGLVLRTIPQRIPGFDELGLPPVLRDIALAQRGLVIFVGGTGTGKTTSLASMVDFRNENTYG
HIITVEDPIEFVHAHKNCIVSQREVGIDTESWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAIVSQRLLPLKERKGRVPAVEILLNSPLIADLVFKGNIPEIKEIMKRSRELG
MQTFDQALFGLYEDGRISYEDALRNADSVNDLRLQIKLHSKHGDHDVSSGIHNLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=284214 Tharo_RS02370 WP_107219840.1 487448..488581(-) (pilU) [Thauera aromatica K172]
ATGGAACGCGACCAGTCCCTCAAGTTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCGGACCTGTTCAT
CACTGCGGGCTTTCCGCCGGCGATCAAGATCGACGGCCGCATCGTGCCGCAATCGAACCAGCTGCTGCAGCCCCAGCACA
CCGCCGAACTGGCGCGCGCAGTGATGAGCGACCGCCAGGCGGCCGAATTCGAAACGCACAAGGAATGCAATTTCGGCATC
TCGCCCGCCGGCATCGGCCGCTTCCGTGCCAACGCCTACATCCAGCAGGGCAAGGTCGGACTGGTGTTGCGCACGATCCC
GCAGCGCATTCCCGGTTTCGACGAGCTCGGCCTGCCGCCGGTGCTGCGCGACATCGCCCTGGCCCAGCGCGGGCTGGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACCACGTCACTGGCCTCGATGGTCGATTTCCGCAACGAGAACACCTACGGC
CACATCATCACGGTCGAAGACCCGATCGAGTTCGTCCATGCGCACAAGAACTGCATCGTCTCCCAGCGCGAAGTCGGTAT
CGACACCGAGTCCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTCATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGACTACGCCATCGCCTTCGCCGAGACCGGCCATCTGTGCCTGGCCACGCTGCATGCCAACAGCGCC
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCGATCGTCTCCCAGCGCCTGCTGCCGCTGAAGGAGCGCAAGGGCCGGGTTCCGGCGGTGGAGATCCTGCTCAACT
CGCCGCTGATCGCCGACCTCGTGTTCAAGGGCAACATTCCCGAGATCAAGGAAATCATGAAGCGCTCGCGTGAGCTGGGC
ATGCAGACGTTCGACCAGGCCCTGTTCGGGCTCTACGAGGACGGTCGGATCAGCTACGAAGACGCGCTGCGCAACGCCGA
CTCGGTCAACGATCTGCGCCTGCAGATCAAGCTCCACAGCAAGCACGGCGACCACGACGTCAGCAGCGGCATCCACAACC
TCGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.155

96.021

0.597
  pilU Acinetobacter baylyi ADP1

58.405

93.103

0.544
  pilU Vibrio cholerae strain A1552

54.722

95.491

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.859

0.401
  pilT Legionella pneumophila strain Lp02

42.598

87.798

0.374
  pilT Legionella pneumophila strain ERS1305867

42.598

87.798

0.374
  pilT Acinetobacter baylyi ADP1

41.246

89.39

0.369
  pilT Acinetobacter baumannii D1279779

41.088

87.798

0.361
  pilT Acinetobacter baumannii strain A118

41.088

87.798

0.361
  pilT Acinetobacter nosocomialis M2

41.088

87.798

0.361
  pilT Pseudomonas aeruginosa PAK

40.597

88.859

0.361
  pilT Pseudomonas stutzeri DSM 10701

40.597

88.859

0.361

Multiple sequence alignment