Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C7R88_RS13755 Genome accession   NZ_CP027852
Coordinates   3055843..3056943 (+) Length   366 a.a.
NCBI ID   WP_010864554.1    Uniprot ID   R8AMI2
Organism   Plesiomonas shigelloides strain MS-17-188     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3050843..3061943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7R88_RS13725 (C7R88_13705) - 3051973..3052578 (-) 606 WP_106911844.1 XTP/dITP diphosphatase -
  C7R88_RS13730 (C7R88_13710) - 3052758..3053261 (-) 504 WP_106911845.1 DUF4426 domain-containing protein -
  C7R88_RS13735 (C7R88_13715) yggU 3053330..3053617 (-) 288 WP_010864558.1 DUF167 family protein YggU -
  C7R88_RS13740 (C7R88_13720) - 3053629..3054183 (-) 555 WP_010864557.1 YggT family protein -
  C7R88_RS13745 (C7R88_13725) proC 3054212..3055030 (-) 819 WP_106911846.1 pyrroline-5-carboxylate reductase -
  C7R88_RS13750 (C7R88_13730) - 3055113..3055820 (-) 708 WP_039045867.1 YggS family pyridoxal phosphate-dependent enzyme -
  C7R88_RS13755 (C7R88_13735) pilT 3055843..3056943 (+) 1101 WP_010864554.1 type IV pilus twitching motility protein PilT Machinery gene
  C7R88_RS13760 (C7R88_13740) pilU 3056927..3058036 (+) 1110 WP_010864553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C7R88_RS13765 (C7R88_13745) npr 3058269..3058544 (+) 276 WP_010864552.1 PTS phosphocarrier protein NPr -
  C7R88_RS13770 (C7R88_13750) ruvX 3058561..3058980 (-) 420 WP_010864551.1 Holliday junction resolvase RuvX -
  C7R88_RS13775 (C7R88_13755) - 3059005..3059568 (-) 564 WP_010864550.1 YqgE/AlgH family protein -
  C7R88_RS13785 (C7R88_13765) gshB 3059782..3060735 (-) 954 WP_047708158.1 glutathione synthase -
  C7R88_RS13790 (C7R88_13770) rsmE 3060745..3061479 (-) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40421.07 Da        Isoelectric Point: 6.7432

>NTDB_id=280956 C7R88_RS13755 WP_010864554.1 3055843..3056943(+) (pilT) [Plesiomonas shigelloides strain MS-17-188]
MDITELLAFSVKHNASDLHLSAGNPPLIRVDGEVRRVNMPVLDGREVRRLVYDIMNDVQRRDFETEMELDFSFELNGVGR
FRVNAYQQQRGISAALRTIPTDIPTLEQLNAPEVFRNIAMQPRGLVLVTGPTGSGKSTTLAAMLNYVNQHMHHHILTIED
PIEFIHTSNKCLINQREVHRHTHSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPGAEKDMVRSMLSESLKAVISQVLLRKEGGGRVAAHEIMLGTPAIRNLIREDKIHQIYSILQTSSRQGMQTLEQAL
NVLTSQGVVSYEEAQRKMNSLMDGGIQNGNTAGGTGAGAMGYGFAG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=280956 C7R88_RS13755 WP_010864554.1 3055843..3056943(+) (pilT) [Plesiomonas shigelloides strain MS-17-188]
ATGGACATAACAGAACTGTTGGCTTTTAGTGTAAAACATAATGCCTCGGATCTGCACCTATCTGCCGGTAACCCACCGCT
GATCCGCGTCGACGGGGAAGTGCGTCGGGTCAATATGCCGGTCCTGGATGGCCGTGAAGTGCGCCGTCTGGTGTATGACA
TTATGAACGATGTTCAGCGCCGTGACTTTGAAACTGAGATGGAACTGGACTTTTCGTTCGAACTGAACGGGGTTGGCCGT
TTCCGGGTTAATGCTTATCAGCAGCAACGGGGAATTTCGGCGGCACTGCGTACCATACCTACAGATATACCAACACTGGA
GCAGTTAAACGCACCGGAAGTGTTTCGGAATATTGCCATGCAGCCGCGTGGACTGGTATTGGTGACCGGCCCGACCGGCT
CGGGGAAGTCGACCACCTTGGCGGCGATGCTCAATTATGTCAATCAGCACATGCATCACCACATTCTGACCATTGAAGAT
CCGATCGAATTTATCCACACCAGTAACAAGTGCCTGATTAACCAGCGTGAGGTGCATCGGCATACCCACAGTTTTAATAA
TGCACTGCGCTCGGCGCTGCGTGAAGACCCGGATGTGATTCTGGTGGGTGAGCTGCGCGATCTGGAAACCATTCGTCTGG
CACTGACTGCCGCCGAAACCGGCCACTTGGTGTTTGGCACCTTGCACACCAGCTCGGCGGCCAAAACCATTGACCGTATT
GTGGATGTGTTCCCCGGCGCGGAAAAAGACATGGTGCGCTCGATGCTGTCTGAATCGCTCAAAGCGGTTATCTCGCAGGT
TTTGCTGCGTAAAGAAGGTGGCGGACGTGTGGCGGCGCACGAGATCATGCTGGGAACGCCAGCGATTCGTAACTTGATCC
GCGAAGATAAAATTCACCAGATCTATTCGATTTTGCAGACCAGCTCGCGCCAAGGCATGCAGACGCTGGAGCAGGCGCTG
AATGTGCTGACCAGTCAGGGCGTGGTCAGCTACGAAGAGGCGCAGCGCAAGATGAACTCGTTGATGGATGGCGGGATCCA
AAACGGCAATACCGCGGGTGGCACCGGTGCAGGAGCGATGGGCTATGGTTTCGCTGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8AMI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.373

92.35

0.678
  pilT Vibrio cholerae strain A1552

73.373

92.35

0.678
  pilT Pseudomonas stutzeri DSM 10701

71.217

92.077

0.656
  pilT Acinetobacter nosocomialis M2

71.343

91.53

0.653
  pilT Acinetobacter baumannii D1279779

71.045

91.53

0.65
  pilT Acinetobacter baumannii strain A118

71.045

91.53

0.65
  pilT Pseudomonas aeruginosa PAK

70.623

92.077

0.65
  pilT Acinetobacter baylyi ADP1

70.448

91.53

0.645
  pilT Neisseria meningitidis 8013

64.793

92.35

0.598
  pilT Neisseria gonorrhoeae MS11

64.497

92.35

0.596
  pilT Legionella pneumophila strain Lp02

65.269

91.257

0.596
  pilT Legionella pneumophila strain ERS1305867

65.269

91.257

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.025

98.087

0.481
  pilU Vibrio cholerae strain A1552

43.023

93.989

0.404
  pilU Pseudomonas stutzeri DSM 10701

43.284

91.53

0.396
  pilU Acinetobacter baylyi ADP1

42.274

93.716

0.396

Multiple sequence alignment