Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C7R88_RS13760 Genome accession   NZ_CP027852
Coordinates   3056927..3058036 (+) Length   369 a.a.
NCBI ID   WP_010864553.1    Uniprot ID   R8AMH9
Organism   Plesiomonas shigelloides strain MS-17-188     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3051927..3063036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7R88_RS13725 (C7R88_13705) - 3051973..3052578 (-) 606 WP_106911844.1 XTP/dITP diphosphatase -
  C7R88_RS13730 (C7R88_13710) - 3052758..3053261 (-) 504 WP_106911845.1 DUF4426 domain-containing protein -
  C7R88_RS13735 (C7R88_13715) yggU 3053330..3053617 (-) 288 WP_010864558.1 DUF167 family protein YggU -
  C7R88_RS13740 (C7R88_13720) - 3053629..3054183 (-) 555 WP_010864557.1 YggT family protein -
  C7R88_RS13745 (C7R88_13725) proC 3054212..3055030 (-) 819 WP_106911846.1 pyrroline-5-carboxylate reductase -
  C7R88_RS13750 (C7R88_13730) - 3055113..3055820 (-) 708 WP_039045867.1 YggS family pyridoxal phosphate-dependent enzyme -
  C7R88_RS13755 (C7R88_13735) pilT 3055843..3056943 (+) 1101 WP_010864554.1 type IV pilus twitching motility protein PilT Machinery gene
  C7R88_RS13760 (C7R88_13740) pilU 3056927..3058036 (+) 1110 WP_010864553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C7R88_RS13765 (C7R88_13745) npr 3058269..3058544 (+) 276 WP_010864552.1 PTS phosphocarrier protein NPr -
  C7R88_RS13770 (C7R88_13750) ruvX 3058561..3058980 (-) 420 WP_010864551.1 Holliday junction resolvase RuvX -
  C7R88_RS13775 (C7R88_13755) - 3059005..3059568 (-) 564 WP_010864550.1 YqgE/AlgH family protein -
  C7R88_RS13785 (C7R88_13765) gshB 3059782..3060735 (-) 954 WP_047708158.1 glutathione synthase -
  C7R88_RS13790 (C7R88_13770) rsmE 3060745..3061479 (-) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C7R88_RS13795 (C7R88_13775) - 3061661..3062173 (+) 513 WP_106911848.1 MarR family winged helix-turn-helix transcriptional regulator -
  C7R88_RS13800 (C7R88_13780) - 3062170..3062379 (+) 210 WP_010864545.1 DUF1656 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41485.52 Da        Isoelectric Point: 5.7325

>NTDB_id=280957 C7R88_RS13760 WP_010864553.1 3056927..3058036(+) (pilU) [Plesiomonas shigelloides strain MS-17-188]
MVSLDELLQEMLRQQASDLYVSVDVPAQLRINHELHPLGEALSIETVTELVAQAMGAERFLQFCQQKEGNMALVRPFARF
RVSGFWQRELPGMVIRHIRTDIPTIEELGLPMLTGELAMTRKGLILVVGATGSGKTTTLAAMLGYRNEFDHSHILTVEDP
IEFIHPHKRCLITQREVGMDTESYEVALRNALRQAPDVIQVGEIRTRDTLQHAIGFSETGHLCIATLHANNASQALERMV
HMIPEGSREAFLFDLSNNLRAIIGQQLVPGITPGRRLLVHEILINTPRVTDLLRKNQLYELRDVMASNSEIGMCTFDQSL
YGLYRRGLISYTDALHYAESANDLRLRIRSSGDEPFSPNTFDDVTVDLS

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=280957 C7R88_RS13760 WP_010864553.1 3056927..3058036(+) (pilU) [Plesiomonas shigelloides strain MS-17-188]
ATGGTTTCGCTGGATGAGTTATTGCAGGAGATGCTGCGCCAGCAGGCGTCGGATCTGTATGTTTCGGTCGATGTACCCGC
GCAGTTGCGCATCAATCATGAGCTGCACCCACTAGGTGAGGCGCTGTCGATTGAAACGGTCACCGAGCTGGTGGCGCAGG
CGATGGGCGCCGAGCGCTTCTTACAGTTTTGTCAGCAAAAAGAGGGCAATATGGCGCTGGTGCGTCCGTTTGCCCGTTTT
CGGGTCTCCGGTTTTTGGCAGCGTGAATTGCCGGGCATGGTGATCCGTCATATTCGTACCGATATTCCGACCATTGAAGA
GCTGGGCTTACCGATGTTAACCGGTGAGCTGGCGATGACCCGCAAAGGTTTGATTTTGGTGGTCGGCGCTACCGGCAGCG
GGAAAACCACCACGCTGGCAGCGATGCTCGGCTATCGCAACGAGTTTGACCATTCGCATATTCTGACGGTGGAAGACCCG
ATTGAATTTATCCATCCGCATAAACGCTGTTTGATCACTCAGCGTGAAGTGGGGATGGATACCGAAAGCTATGAAGTCGC
CCTGCGTAATGCGCTGCGCCAAGCACCGGATGTGATTCAGGTTGGCGAGATCCGCACCCGCGACACGCTGCAACATGCGA
TTGGCTTTTCCGAAACCGGCCATCTGTGTATCGCCACCTTGCACGCCAATAACGCCAGCCAAGCGCTGGAGCGGATGGTA
CACATGATCCCTGAAGGTAGCCGCGAAGCTTTCTTGTTTGATTTATCCAACAACTTGCGCGCCATTATCGGGCAGCAGCT
GGTGCCGGGGATCACCCCCGGGCGCCGTTTGCTGGTGCACGAGATTTTGATCAACACGCCGCGTGTGACCGATTTGCTGC
GTAAAAATCAGTTGTATGAGCTGCGTGACGTGATGGCCAGTAACAGCGAGATTGGCATGTGTACGTTTGACCAATCGCTG
TATGGCTTGTATCGGCGCGGGCTGATTAGCTATACCGATGCGCTGCACTATGCGGAGTCAGCTAACGATTTGCGTTTGCG
GATCCGCAGTAGCGGTGATGAGCCGTTCTCACCGAACACCTTCGATGATGTGACGGTGGATTTAAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8AMH9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

54.768

99.458

0.545
  pilU Pseudomonas stutzeri DSM 10701

51.275

95.664

0.491
  pilU Acinetobacter baylyi ADP1

46.742

95.664

0.447
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.344

99.187

0.39

Multiple sequence alignment