Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C7H73_RS00400 Genome accession   NZ_CP027792
Coordinates   94690..95733 (+) Length   347 a.a.
NCBI ID   WP_106844836.1    Uniprot ID   A0A2P1NGX8
Organism   Pulveribacter suum strain SC2-7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 89690..100733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7H73_RS00385 (C7H73_00385) - 91551..93158 (-) 1608 Protein_77 AMP-binding protein -
  C7H73_RS00390 (C7H73_00390) - 93234..93935 (+) 702 WP_227001375.1 TetR/AcrR family transcriptional regulator -
  C7H73_RS00395 (C7H73_00395) - 93920..94645 (-) 726 WP_106844835.1 YggS family pyridoxal phosphate-dependent enzyme -
  C7H73_RS00400 (C7H73_00400) pilT 94690..95733 (+) 1044 WP_106844836.1 type IV pilus twitching motility protein PilT Machinery gene
  C7H73_RS00405 (C7H73_00405) pilU 95786..96922 (+) 1137 WP_106847472.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C7H73_RS00410 (C7H73_00410) - 97012..97947 (+) 936 WP_106844837.1 NAD(P)-dependent oxidoreductase -
  C7H73_RS00415 (C7H73_00415) - 97960..98628 (-) 669 WP_106844838.1 BON domain-containing protein -
  C7H73_RS00420 (C7H73_00420) - 98625..99221 (-) 597 WP_106844839.1 SIS domain-containing protein -
  C7H73_RS00425 (C7H73_00425) - 99251..99652 (-) 402 WP_106844840.1 YraN family protein -
  C7H73_RS00430 (C7H73_00430) rsmI 99652..100569 (+) 918 WP_106844841.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38134.84 Da        Isoelectric Point: 6.8925

>NTDB_id=280779 C7H73_RS00400 WP_106844836.1 94690..95733(+) (pilT) [Pulveribacter suum strain SC2-7]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRLNVDALDHKTVHAMVYDIMTDSQRKVYEEFLEVDFSFEIDGLAR
FRVNAFNHYRGAGAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNESEYGHILTVED
PIEFVHESKKSLINQREVGPMTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTGNSVGMQTLDQN
LADLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=280779 C7H73_RS00400 WP_106844836.1 94690..95733(+) (pilT) [Pulveribacter suum strain SC2-7]
GTGGACATCACCCAGCTGCTTGCCTTCAGTGTCAAGAACAAGGCCTCGGACCTGCACCTGTCCGCCGGGCTGCCGCCCAT
GATCCGCGTGCATGGCGACGTGCGCCGCCTGAACGTCGATGCGCTGGACCACAAGACGGTGCACGCCATGGTGTACGACA
TCATGACGGACTCGCAGCGCAAGGTGTACGAGGAGTTCCTGGAGGTGGACTTCTCCTTCGAGATCGACGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCACTACCGCGGCGCAGGCGCGGTGTTCCGGACCATTCCCAGCAAGATCCTCACGCTGGA
GCAGCTGAATGCGCCCAAGATCTTCGCCGACCTGGCGCTCAAGCCCCGGGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGGTCAACCACCTGAACGAGAGCGAGTACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCGTTCTCCGC
CGCGCTGCGCTCGGCCCTGCGCGAGGACCCGGACGCCATCCTGGTGGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CCATGACGGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACGTCGAGTGCCGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAGATGGTGCGGGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
ATTGTGCAAGACCAAGGACGGCTCGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAC
CTGGCCGACCTGGTGCGGCGCAACATCATCAGCCCGGCCGAGGCCCGCAGCAAGGCCAAGATCCCGGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P1NGX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

72.566

97.695

0.709
  pilT Legionella pneumophila strain ERS1305867

72.566

97.695

0.709
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389

Multiple sequence alignment