Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C7H73_RS00405 Genome accession   NZ_CP027792
Coordinates   95786..96922 (+) Length   378 a.a.
NCBI ID   WP_106847472.1    Uniprot ID   A0A2P1NPD5
Organism   Pulveribacter suum strain SC2-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 90786..101922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7H73_RS00385 (C7H73_00385) - 91551..93158 (-) 1608 Protein_77 AMP-binding protein -
  C7H73_RS00390 (C7H73_00390) - 93234..93935 (+) 702 WP_227001375.1 TetR/AcrR family transcriptional regulator -
  C7H73_RS00395 (C7H73_00395) - 93920..94645 (-) 726 WP_106844835.1 YggS family pyridoxal phosphate-dependent enzyme -
  C7H73_RS00400 (C7H73_00400) pilT 94690..95733 (+) 1044 WP_106844836.1 type IV pilus twitching motility protein PilT Machinery gene
  C7H73_RS00405 (C7H73_00405) pilU 95786..96922 (+) 1137 WP_106847472.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C7H73_RS00410 (C7H73_00410) - 97012..97947 (+) 936 WP_106844837.1 NAD(P)-dependent oxidoreductase -
  C7H73_RS00415 (C7H73_00415) - 97960..98628 (-) 669 WP_106844838.1 BON domain-containing protein -
  C7H73_RS00420 (C7H73_00420) - 98625..99221 (-) 597 WP_106844839.1 SIS domain-containing protein -
  C7H73_RS00425 (C7H73_00425) - 99251..99652 (-) 402 WP_106844840.1 YraN family protein -
  C7H73_RS00430 (C7H73_00430) rsmI 99652..100569 (+) 918 WP_106844841.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  C7H73_RS00440 (C7H73_00440) rplQ 100755..101156 (-) 402 WP_106844842.1 50S ribosomal protein L17 -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42053.47 Da        Isoelectric Point: 6.9079

>NTDB_id=280780 C7H73_RS00405 WP_106847472.1 95786..96922(+) (pilU) [Pulveribacter suum strain SC2-7]
MERDQASKFINDLLRLMVNRGGSDLFITAEFPPAIKVDGKITKVSPQPLTPAHTLTLARSIMSDRQIADFERTKECNFAI
SPAGIGRFRVNAFIQQSRVGMVLRSIPTTPPTIDGLGMPQVLKEVVMTKRGLCILVGATGSGKSTTLAAMLDWRNENTFG
HIITVEDPVEFVHPHKNCVVTQREIGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFSETGHLCLATLHANSA
NQALDRIINFFPEERRTQLLMDLSLNLRAMVSQRLLPRQDSKGRAAAVEIMLNTPLIADLIFKGDVTEIKEIMKKSRNLG
MQTFDQSLFDLFEANVITYEDALRNADSVNDLRLQIKLSSQRAKTTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=280780 C7H73_RS00405 WP_106847472.1 95786..96922(+) (pilU) [Pulveribacter suum strain SC2-7]
ATGGAACGCGATCAGGCGAGCAAATTCATCAACGACCTGCTCCGGCTGATGGTCAACCGCGGCGGCAGCGACCTGTTCAT
CACGGCGGAGTTTCCGCCCGCCATCAAGGTGGACGGCAAGATCACCAAGGTCTCGCCCCAGCCGCTCACGCCCGCGCACA
CGCTGACACTGGCGCGCTCCATCATGAGCGACCGCCAGATTGCCGACTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCGCCGGCCGGCATCGGGCGCTTCCGGGTCAACGCCTTCATCCAGCAAAGCCGCGTGGGCATGGTGCTGCGCTCCATCCC
CACCACGCCGCCGACCATCGACGGGCTGGGCATGCCGCAGGTGCTCAAGGAGGTCGTCATGACCAAGCGCGGGCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCCACCACGCTGGCGGCCATGCTGGACTGGCGCAACGAGAACACCTTCGGC
CACATCATCACCGTGGAGGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCGTGGTCACCCAGCGCGAGATCGGGCT
GGACACGGACAGCTGGGAAGCGGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGAGCACGCGGTGGCCTTCTCCGAGACCGGCCACCTGTGCCTGGCCACACTGCACGCCAACAGCGCC
AACCAGGCGTTGGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCACCCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCGCAGCGCTTGCTGCCGCGCCAGGACAGCAAGGGCCGCGCCGCCGCCGTGGAGATCATGCTCAACA
CTCCCTTGATCGCCGACCTCATCTTCAAGGGCGACGTCACCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGTCACTGTTCGACCTGTTCGAGGCAAACGTCATCACCTACGAGGACGCGCTGCGCAATGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAGCAGCCAGCGCGCCAAGACCACCGACCTGGCCGCGGGCACCGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P1NPD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

56.145

94.709

0.532
  pilU Vibrio cholerae strain A1552

52.874

92.063

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Acinetobacter baylyi ADP1

41.768

86.772

0.362

Multiple sequence alignment