Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4F17_RS21895 Genome accession   NZ_CP027773
Coordinates   4698141..4699184 (-) Length   347 a.a.
NCBI ID   WP_081266042.1    Uniprot ID   -
Organism   Variovorax sp. PMC12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4693141..4704184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4F17_RS21875 (C4F17_21875) - 4693825..4694733 (-) 909 WP_106936637.1 NAD(P)-dependent oxidoreductase -
  C4F17_RS21880 (C4F17_21880) - 4694821..4695303 (+) 483 WP_106936638.1 MerR family transcriptional regulator -
  C4F17_RS21885 (C4F17_21885) - 4695300..4696850 (+) 1551 WP_106936639.1 MFS transporter -
  C4F17_RS21890 (C4F17_21890) pilU 4696868..4698004 (-) 1137 WP_081266043.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C4F17_RS21895 (C4F17_21895) pilT 4698141..4699184 (-) 1044 WP_081266042.1 type IV pilus twitching motility protein PilT Machinery gene
  C4F17_RS21900 (C4F17_21900) - 4699236..4699946 (+) 711 WP_106936640.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4F17_RS21905 (C4F17_21905) - 4699951..4700418 (-) 468 WP_081266040.1 Lrp/AsnC ligand binding domain-containing protein -
  C4F17_RS21910 (C4F17_21910) - 4700563..4700985 (-) 423 WP_106936641.1 hypothetical protein -
  C4F17_RS21915 (C4F17_21915) - 4701216..4702490 (+) 1275 WP_081266038.1 Glu/Leu/Phe/Val dehydrogenase -
  C4F17_RS21920 (C4F17_21920) - 4702534..4703055 (+) 522 WP_106936642.1 alpha-ketoglutarate-dependent dioxygenase AlkB -
  C4F17_RS21925 (C4F17_21925) - 4703081..4703734 (+) 654 WP_106936643.1 nucleoside/nucleotide kinase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38161.83 Da        Isoelectric Point: 6.8354

>NTDB_id=280493 C4F17_RS21895 WP_081266042.1 4698141..4699184(-) (pilT) [Variovorax sp. PMC12]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGQGMQTLDQN
LTDLVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=280493 C4F17_RS21895 WP_081266042.1 4698141..4699184(-) (pilT) [Variovorax sp. PMC12]
GTGGACATCACCCAACTGCTGGCATTCAGCGTCAAGAACAAAGCCTCCGACCTGCATCTTTCCGCGGGCCTGCCGCCCAT
GATCCGCGTGAACGGCGACGTGCGCCGCATCAACGTCGACGCGCTCGACCACAAGGGCGTGCATGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTCGAGGTCGACTTTTCGTTCGAGATCGACGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGGCACGCGGCGCGGCGGCGGTGTTCCGGACCATTCCCTCGAAGATCCTGACGCTGGA
GCAGCTGAACGCGCCCAAGATTTTCGGTGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGTCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAACTACCTCAATGAAAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGACCGATGACGCTGTCGTTCTCGAA
CGCGCTGCGCTCCGCACTGCGCGAAGACCCGGACGCGATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAAAAGGAAATGATCCGCGCGATGCTGTCGGAGTCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGAAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCCAGGGCATGCAGACGCTCGACCAGAAC
CTGACGGATCTGGTGCGGCGCAATACCATCTCGGCTGCGGAAGCCCGCGGCAAGGCCAAGATCCCCGAAAACTTCCCCGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

66.957

99.424

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment