Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C4F17_RS21890 Genome accession   NZ_CP027773
Coordinates   4696868..4698004 (-) Length   378 a.a.
NCBI ID   WP_081266043.1    Uniprot ID   -
Organism   Variovorax sp. PMC12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4691868..4703004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4F17_RS21860 (C4F17_21860) - 4691879..4692247 (+) 369 WP_081266049.1 YraN family protein -
  C4F17_RS21865 (C4F17_21865) - 4692334..4692978 (+) 645 WP_081266048.1 SIS domain-containing protein -
  C4F17_RS21870 (C4F17_21870) - 4692975..4693781 (+) 807 WP_106936636.1 BON domain-containing protein -
  C4F17_RS21875 (C4F17_21875) - 4693825..4694733 (-) 909 WP_106936637.1 NAD(P)-dependent oxidoreductase -
  C4F17_RS21880 (C4F17_21880) - 4694821..4695303 (+) 483 WP_106936638.1 MerR family transcriptional regulator -
  C4F17_RS21885 (C4F17_21885) - 4695300..4696850 (+) 1551 WP_106936639.1 MFS transporter -
  C4F17_RS21890 (C4F17_21890) pilU 4696868..4698004 (-) 1137 WP_081266043.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C4F17_RS21895 (C4F17_21895) pilT 4698141..4699184 (-) 1044 WP_081266042.1 type IV pilus twitching motility protein PilT Machinery gene
  C4F17_RS21900 (C4F17_21900) - 4699236..4699946 (+) 711 WP_106936640.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4F17_RS21905 (C4F17_21905) - 4699951..4700418 (-) 468 WP_081266040.1 Lrp/AsnC ligand binding domain-containing protein -
  C4F17_RS21910 (C4F17_21910) - 4700563..4700985 (-) 423 WP_106936641.1 hypothetical protein -
  C4F17_RS21915 (C4F17_21915) - 4701216..4702490 (+) 1275 WP_081266038.1 Glu/Leu/Phe/Val dehydrogenase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41812.98 Da        Isoelectric Point: 6.4178

>NTDB_id=280492 C4F17_RS21890 WP_081266043.1 4696868..4698004(-) (pilU) [Variovorax sp. PMC12]
MERDQASQFINDLLKLMVSRAGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDSLGMPQVLKEVTMSKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=280492 C4F17_RS21890 WP_081266043.1 4696868..4698004(-) (pilU) [Variovorax sp. PMC12]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCGCCGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCTGCCATCAAGGTCGACGGCAAGGTGACCAAGGTGTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCCACCATCGACAGCCTGGGCATGCCCCAGGTGCTGAAGGAAGTGACGATGAGCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAATCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAACTCGTTCGGC
CACATCGTGACGGTGGAGGACCCGGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAACACGCGGTGGCCTTCGCCGAGACCGGCCACCTTTGCATGGCGACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAATTTCTTCCCCGAAGAGCGCCGCGCGCAGTTGCTGATGGACCTGTCGCTGAACCT
GCGTTCGCTGGTGTCGCAGCGGCTGGTGCCCACCGAGGACGGCCTGGGCCGCGTGGCGGCGGTCGAGGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACCTTCGAGGACGCGATCCGCAACGCCGA
CTCCGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCCGCCGGCACGGAGC
ATTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain ERS1305867

43.769

87.037

0.381
  pilT Legionella pneumophila strain Lp02

43.769

87.037

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

44.201

84.392

0.373
  pilT Acinetobacter baumannii D1279779

44.201

84.392

0.373
  pilT Acinetobacter baylyi ADP1

42.724

85.45

0.365

Multiple sequence alignment