Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C6571_RS09135 Genome accession   NZ_CP027669
Coordinates   1966188..1967231 (-) Length   347 a.a.
NCBI ID   WP_106446411.1    Uniprot ID   A0A2S0MZW1
Organism   Simplicispira suum strain SC1-8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1961188..1972231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6571_RS09105 (C6571_09105) - 1961519..1961914 (+) 396 WP_106446405.1 YraN family protein -
  C6571_RS09110 (C6571_09110) - 1962001..1962594 (+) 594 WP_106446406.1 SIS domain-containing protein -
  C6571_RS09115 (C6571_09115) - 1962591..1963247 (+) 657 WP_106446407.1 BON domain-containing protein -
  C6571_RS09120 (C6571_09120) - 1963301..1964215 (-) 915 WP_106446408.1 NAD(P)-dependent oxidoreductase -
  C6571_RS09125 (C6571_09125) pilU 1964268..1965404 (-) 1137 WP_106446409.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C6571_RS09130 (C6571_09130) - 1965502..1966134 (-) 633 WP_106446410.1 cyclic nucleotide-binding domain-containing protein -
  C6571_RS09135 (C6571_09135) pilT 1966188..1967231 (-) 1044 WP_106446411.1 type IV pilus twitching motility protein PilT Machinery gene
  C6571_RS09140 (C6571_09140) - 1967270..1967986 (+) 717 WP_106446412.1 YggS family pyridoxal phosphate-dependent enzyme -
  C6571_RS09145 (C6571_09145) - 1967993..1968556 (-) 564 WP_106446413.1 DUF3617 domain-containing protein -
  C6571_RS09150 (C6571_09150) - 1968801..1969940 (+) 1140 WP_106446414.1 aminotransferase class V-fold PLP-dependent enzyme -
  C6571_RS09155 (C6571_09155) - 1969999..1970421 (+) 423 WP_106446415.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  C6571_RS09160 (C6571_09160) - 1970431..1971714 (+) 1284 WP_106446416.1 DUF445 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38166.90 Da        Isoelectric Point: 6.8354

>NTDB_id=278858 C6571_RS09135 WP_106446411.1 1966188..1967231(-) (pilT) [Simplicispira suum strain SC1-8]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDSQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLSLKPRGLVLVTGPTGSGKSTTLAGMVNHLNESEYGHVLTIED
PIEFVHESKKCLINQREVGPMTLSFAAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=278858 C6571_RS09135 WP_106446411.1 1966188..1967231(-) (pilT) [Simplicispira suum strain SC1-8]
GTGGACATCACCCAGCTGCTCGCATTCAGCGTCAAAAACAAGGCGTCCGACCTGCACCTCTCTGCGGGCCTTCCGCCGAT
GATCCGGGTGCACGGCGACGTGCGGCGCATCAACGTCGATGCGCTGGACCACAAAACGGTTCACGCCATGGTGTACGACA
TCATGAGCGACTCGCAGCGCAAGACCTACGAAGAATTCCTGGAAGTCGACTTCTCGTTCGAGATCGAAGGTCTGGCCCGT
TTTCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGCCGCCGTGTTCCGAACGATTCCGAGCAAGATCCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATTTTTGCCGACCTCTCGCTCAAACCGCGCGGACTGGTACTGGTGACGGGCCCCACGGGCT
CGGGCAAATCGACCACGCTGGCCGGCATGGTCAACCACCTCAACGAGAGCGAATACGGCCACGTGCTCACTATCGAGGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTCGCCGC
CGCCCTGCGCTCAGCGCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAAATGCGCGATCTGGAAACGATTCGGCTGG
CGATGACTGCGGCAGAAACCGGGCACCTGGTGTTCGGCACGCTGCACACCTCGAGCGCTGCAAAGACCATCGACCGGATC
ATCGACGTGTTCCCAGCCGAAGAAAAGGAAATGGTCCGGGCCATGCTGTCCGAGTCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGATCGGGCCGAGTTGCGGCGCACGAGATCATGCTGGGCACCAGTGCCATCCGCAACCTCA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATTCAGACCAGCAATAGCGTGGGTATGCAGACGCTGGACCAGAAT
CTGACCGACTTGGTACGCCGCAACATCATCAGCCCCGCCGAGGCACGCAGCAAAGCCAAGATTCCAGACAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S0MZW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

72.861

97.695

0.712
  pilT Legionella pneumophila strain ERS1305867

72.861

97.695

0.712
  pilT Neisseria meningitidis 8013

67.647

97.983

0.663
  pilT Neisseria gonorrhoeae MS11

67.353

97.983

0.66
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.862

98.271

0.657
  pilT Vibrio cholerae strain A1552

66.862

98.271

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389

Multiple sequence alignment