Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C6571_RS09125 Genome accession   NZ_CP027669
Coordinates   1964268..1965404 (-) Length   378 a.a.
NCBI ID   WP_106446409.1    Uniprot ID   A0A2S0MZV2
Organism   Simplicispira suum strain SC1-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1959268..1970404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6571_RS09100 (C6571_09100) rsmI 1960596..1961519 (-) 924 WP_106446404.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  C6571_RS09105 (C6571_09105) - 1961519..1961914 (+) 396 WP_106446405.1 YraN family protein -
  C6571_RS09110 (C6571_09110) - 1962001..1962594 (+) 594 WP_106446406.1 SIS domain-containing protein -
  C6571_RS09115 (C6571_09115) - 1962591..1963247 (+) 657 WP_106446407.1 BON domain-containing protein -
  C6571_RS09120 (C6571_09120) - 1963301..1964215 (-) 915 WP_106446408.1 NAD(P)-dependent oxidoreductase -
  C6571_RS09125 (C6571_09125) pilU 1964268..1965404 (-) 1137 WP_106446409.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C6571_RS09130 (C6571_09130) - 1965502..1966134 (-) 633 WP_106446410.1 cyclic nucleotide-binding domain-containing protein -
  C6571_RS09135 (C6571_09135) pilT 1966188..1967231 (-) 1044 WP_106446411.1 type IV pilus twitching motility protein PilT Machinery gene
  C6571_RS09140 (C6571_09140) - 1967270..1967986 (+) 717 WP_106446412.1 YggS family pyridoxal phosphate-dependent enzyme -
  C6571_RS09145 (C6571_09145) - 1967993..1968556 (-) 564 WP_106446413.1 DUF3617 domain-containing protein -
  C6571_RS09150 (C6571_09150) - 1968801..1969940 (+) 1140 WP_106446414.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41650.05 Da        Isoelectric Point: 6.9660

>NTDB_id=278857 C6571_RS09125 WP_106446409.1 1964268..1965404(-) (pilU) [Simplicispira suum strain SC1-8]
MERDQASKFINDLLKLLVSRGGSDLFITAEFPPAIKVDGSIGKVSPQALTPSHTLTLARSIMSDKQAADFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRVIPLTLPTIDGLGVPQVLKEVTMSKRGLCILVGATGSGKSTTLAAMVDWRNEHSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDTWGAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLLPKQDGKGRAAAVEILLNTPLISDLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANVITYEDALRNADSLNDLRLQIKLSSQRAKTTDLAAGTEHFVIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=278857 C6571_RS09125 WP_106446409.1 1964268..1965404(-) (pilU) [Simplicispira suum strain SC1-8]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACCTGCTGAAGCTGCTTGTCAGCCGTGGCGGCAGTGACCTGTTCAT
CACGGCGGAGTTCCCTCCGGCCATCAAGGTGGACGGCTCGATTGGCAAGGTCTCGCCGCAGGCCCTGACTCCCAGCCACA
CCTTGACGCTGGCGCGTTCGATCATGAGCGACAAGCAAGCAGCCGATTTCGAGCGCACCAAAGAGTGCAACTTCGCCATT
TCGCCTGCCGGAATCGGGCGCTTTCGCGTTAACGCTTTCATTCAGCAAGGGCGCGTTGGCATGGTGCTGCGGGTGATTCC
GCTCACCCTGCCCACCATCGATGGCCTGGGCGTTCCGCAGGTGCTCAAGGAAGTGACCATGTCCAAACGCGGCCTGTGCA
TCCTGGTGGGCGCCACAGGCTCGGGCAAGTCGACCACCCTGGCCGCCATGGTGGATTGGCGCAATGAGCACTCGTTTGGG
CACATCATCACGGTGGAAGACCCGATCGAATTCGTGCACCCCCACAAAAACTGCGTGGTGACGCAGCGCGAAGTGGGACT
CGACACCGACACCTGGGGTGCAGCGCTGAAAAACACTCTGCGCCAGGCGCCCGACGTGATTCTGATGGGTGAAATTCGCG
ATCGGGAGACCATGGAGCACGCCATTGCGTTCTCGGAAACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGTATCATCAACTTCTTCCCGGAAGAGCGGCGCGCGCAGTTGCTGATGGATTTGTCACTCAACCT
TCGGTCGCTGGTTTCGCAGCGCCTTCTGCCCAAGCAGGACGGCAAGGGCCGGGCTGCGGCCGTCGAGATCTTGCTCAACA
CGCCGCTCATTTCCGATCTGATCTTCAAGGGCGAAGTCGCTGAGATCAAAGAGATCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTTGATCTGTTCGAAGCCAACGTCATTACTTACGAAGACGCGCTGCGCAACGCCGA
CTCCCTCAACGACCTGCGGCTGCAGATCAAGCTCAGCAGCCAGCGCGCCAAGACAACCGACTTGGCTGCCGGCACGGAGC
ACTTCGTTATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S0MZV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.464

92.328

0.577
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

53.448

92.063

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368

Multiple sequence alignment