Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   C6Y50_RS21505 Genome accession   NZ_CP027664
Coordinates   2452317..2452754 (-) Length   145 a.a.
NCBI ID   WP_106442873.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain 1W1-1A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2447317..2457754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Y50_RS11575 (C6Y50_11580) - 2448279..2449037 (+) 759 WP_106442871.1 Nif3-like dinuclear metal center hexameric protein -
  C6Y50_RS11580 (C6Y50_11585) cysD 2449226..2450143 (+) 918 WP_011912238.1 sulfate adenylyltransferase subunit CysD -
  C6Y50_RS11585 (C6Y50_11590) cysN 2450155..2452053 (+) 1899 WP_106442872.1 sulfate adenylyltransferase subunit CysN -
  C6Y50_RS21505 (C6Y50_11595) pilA/pilA1 2452317..2452754 (-) 438 WP_106442873.1 pilin Machinery gene
  C6Y50_RS11595 (C6Y50_11600) pilB 2453074..2454777 (+) 1704 WP_106442874.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6Y50_RS11600 (C6Y50_11605) pilC 2454780..2455997 (+) 1218 WP_106442875.1 type II secretion system F family protein Machinery gene
  C6Y50_RS11605 (C6Y50_11610) pilD 2456000..2456869 (+) 870 WP_106442876.1 prepilin peptidase Machinery gene
  C6Y50_RS11610 (C6Y50_11615) coaE 2457001..2457609 (+) 609 WP_106442877.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14849.83 Da        Isoelectric Point: 4.1403

>NTDB_id=278793 C6Y50_RS21505 WP_106442873.1 2452317..2452754(-) (pilA/pilA1) [Stutzerimonas stutzeri strain 1W1-1A]
MKSQMQKGFTLIELMIVVAIIGILAAIALPAYQDYTGRAQASEALSATAGLRADIGVWLSEEGSLPATGSQVDTDAQLLE
GKYFGAGDVEVGSAGDGVIGITFASGVHSGSTMTLTPNLSAGNNQIADWQCDGLAAKFLPSACRP

Nucleotide


Download         Length: 438 bp        

>NTDB_id=278793 C6Y50_RS21505 WP_106442873.1 2452317..2452754(-) (pilA/pilA1) [Stutzerimonas stutzeri strain 1W1-1A]
ATGAAATCGCAGATGCAGAAAGGCTTTACCCTTATCGAACTGATGATCGTCGTTGCGATCATCGGCATTCTGGCCGCCAT
CGCACTGCCGGCTTATCAGGACTACACCGGCCGCGCCCAAGCCTCCGAAGCCCTGAGCGCAACCGCAGGCCTGCGCGCCG
ACATTGGCGTGTGGCTTTCTGAAGAAGGTAGCCTACCCGCTACTGGAAGCCAGGTAGATACCGATGCTCAGTTGCTGGAA
GGCAAATACTTCGGTGCAGGTGACGTAGAAGTTGGTAGCGCTGGCGACGGCGTTATCGGTATCACTTTTGCTAGCGGCGT
ACATAGCGGCTCCACAATGACTCTGACACCTAACCTGAGCGCCGGCAACAATCAAATTGCCGATTGGCAATGTGATGGCC
TGGCCGCGAAATTCCTTCCGTCGGCTTGCCGTCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)

Pilin (bacterial filament) (Pilin)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

50

100

0.531
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.667

100

0.462
  pilA2 Legionella pneumophila str. Paris

44.828

100

0.448
  pilA2 Legionella pneumophila strain ERS1305867

44.828

100

0.448
  pilE Neisseria gonorrhoeae MS11

40.123

100

0.448
  pilA Ralstonia pseudosolanacearum GMI1000

36.095

100

0.421
  pilE Neisseria gonorrhoeae strain FA1090

37.821

100

0.407
  pilA Acinetobacter nosocomialis M2

52.252

76.552

0.4
  pilA Pseudomonas aeruginosa PAK

36.364

100

0.386
  comP Acinetobacter baylyi ADP1

38.356

100

0.386
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36

100

0.372
  pilA Vibrio cholerae strain A1552

36

100

0.372
  pilA Vibrio cholerae C6706

36

100

0.372
  pilA Glaesserella parasuis strain SC1401

35.811

100

0.366

Multiple sequence alignment