Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6Y50_RS11595 Genome accession   NZ_CP027664
Coordinates   2453074..2454777 (+) Length   567 a.a.
NCBI ID   WP_106442874.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain 1W1-1A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2448074..2459777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Y50_RS11575 (C6Y50_11580) - 2448279..2449037 (+) 759 WP_106442871.1 Nif3-like dinuclear metal center hexameric protein -
  C6Y50_RS11580 (C6Y50_11585) cysD 2449226..2450143 (+) 918 WP_011912238.1 sulfate adenylyltransferase subunit CysD -
  C6Y50_RS11585 (C6Y50_11590) cysN 2450155..2452053 (+) 1899 WP_106442872.1 sulfate adenylyltransferase subunit CysN -
  C6Y50_RS21505 (C6Y50_11595) pilA/pilA1 2452317..2452754 (-) 438 WP_106442873.1 pilin Machinery gene
  C6Y50_RS11595 (C6Y50_11600) pilB 2453074..2454777 (+) 1704 WP_106442874.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6Y50_RS11600 (C6Y50_11605) pilC 2454780..2455997 (+) 1218 WP_106442875.1 type II secretion system F family protein Machinery gene
  C6Y50_RS11605 (C6Y50_11610) pilD 2456000..2456869 (+) 870 WP_106442876.1 prepilin peptidase Machinery gene
  C6Y50_RS11610 (C6Y50_11615) coaE 2457001..2457609 (+) 609 WP_106442877.1 dephospho-CoA kinase -
  C6Y50_RS11615 (C6Y50_11620) yacG 2457606..2457803 (+) 198 WP_095460669.1 DNA gyrase inhibitor YacG -
  C6Y50_RS11620 (C6Y50_11625) - 2457816..2458496 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  C6Y50_RS11625 (C6Y50_11630) - 2458553..2459593 (-) 1041 WP_106442878.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62085.04 Da        Isoelectric Point: 5.4725

>NTDB_id=278779 C6Y50_RS11595 WP_106442874.1 2453074..2454777(+) (pilB) [Stutzerimonas stutzeri strain 1W1-1A]
MSENISLSGLARQMVLAGVLDERTAQQAQVQALRNKTSFVTYVVQNKLAKGRAVAEVASEQFGVALLDLAALDKEVPPKD
VVSEKLARQHRLLPLAKRGNKLYVAVSDPANHQAISDIQFSTGLTTEAILVEDDKLGDAIDKFFDNGSTGLEDLGDVDLD
GVDVETVSDDIKGDDGGAEADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEVDVPRDVLLREGFP
EQKLGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPSLQRIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=278779 C6Y50_RS11595 WP_106442874.1 2453074..2454777(+) (pilB) [Stutzerimonas stutzeri strain 1W1-1A]
ATGAGCGAAAATATTTCTCTGTCCGGGTTGGCTCGGCAGATGGTGTTGGCTGGTGTACTGGATGAGCGGACAGCACAGCA
GGCGCAGGTTCAGGCGCTGCGAAACAAAACATCCTTCGTCACCTACGTAGTCCAGAACAAATTGGCAAAGGGGCGTGCCG
TCGCTGAAGTTGCCTCGGAGCAGTTCGGCGTTGCGCTCTTGGATCTGGCGGCTCTGGACAAGGAGGTGCCGCCCAAGGAT
GTCGTCAGCGAGAAGCTGGCTCGCCAGCATCGCTTGCTCCCGCTGGCTAAGCGCGGCAATAAGCTTTACGTGGCCGTTTC
AGATCCGGCGAACCATCAGGCGATTTCCGATATTCAGTTCAGTACTGGGTTAACCACCGAGGCCATCCTCGTCGAGGACG
ACAAGCTCGGGGACGCCATCGATAAATTTTTCGATAATGGTTCAACCGGTCTGGAGGATCTGGGCGACGTGGATCTCGAC
GGAGTGGATGTCGAGACCGTTTCGGATGACATAAAAGGAGACGATGGCGGTGCGGAGGCTGACGATGCGCCGGTCGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATTCGTGGGGGCTCATCAGACCTCCATTTCGAGCCCTATGAAAAGGCCTATC
GAGTTCGGTTGCGTACTGACGGAATTCTGCATGAGGTGGCGCGTCCGCCCATTCAACTGGCGCCTCGAATTTCGGCGCGT
CTAAAGGTCATGGCAGGGCTGGATATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGCATCAAGATGAAACTCTCCAAGAG
CAAGGCCATCGATTTCCGGGTCAACACGCTACCGACCCTGTGGGGCGAAAAGATCGTGATGCGGATTCTTGATCCCTCAA
GTGCTCAAATGGGCATCGATGCGCTGGGGTATGAGGAGGATCAAAAAGAGCTGTATATGAATGCTCTGCGGCAACCGCAG
GGTATGATTCTCGTGACTGGTCCGACGGGCTCCGGTAAGACGGTATCCCTCTACACCGGGTTGAACATCCTCAACACCGT
CGATGTGAACATTTCGACGGCGGAGGATCCGGTAGAGATCAACCTCGAGGGTATCAACCAGGTCAACGTCAATCCTAAAC
AGGGCATGGACTTTTCTCAGGCCCTCCGTGCCTTTCTACGTCAGGACCCGGACATCATCATGGTAGGGGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCTGCGCAGACGGGCCATATGGTGATGTCGACACTTCATACCAATAGCGCTGC
GGAAACCCTGACGCGTCTGCGCAACATGGGGGTGCCTGCATTCAACATCGCGACCTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCACGAAAGCTCTGCAATAGCTGCAAGAAAGAGGTTGATGTCCCCCGTGACGTTTTGCTGCGCGAAGGGTTCCCT
GAGCAGAAGCTTGGAACCTTCAAGATTTATGGTCCGGTAGGTTGCGACAATTGCAAGGGTGGCTACAAGGGGCGCGTCGG
TATTTATGAAGTGGTTAAAAACACGCCATCGTTGCAGCGGATTATCATGGAAGACGGAAACTCCATCGATATATCAACCC
AGATGCGCAAAGACGGCTTCAACGACCTGCGGACCTCGGCTTTGCTGAAAGCCATGCAGGGCGTGACCAGCCTCGAAGAA
GTCAATCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.638

99.471

0.603
  pilB Acinetobacter baylyi ADP1

59.574

99.471

0.593
  pilB Legionella pneumophila strain ERS1305867

55.769

100

0.563
  pilB Vibrio cholerae strain A1552

51.287

95.944

0.492
  pilB Vibrio campbellii strain DS40M4

48.246

100

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

51.923

91.711

0.476
  pilF Neisseria gonorrhoeae MS11

51.731

91.711

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

35.91

100

0.393

Multiple sequence alignment