Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4901_RS14660 Genome accession   NZ_CP027663
Coordinates   2883235..2884305 (-) Length   356 a.a.
NCBI ID   WP_110137958.1    Uniprot ID   A0A2Z3R2Z3
Organism   Acidiferrobacter sp. SPIII_3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2878235..2889305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4901_RS18225 - 2878610..2879467 (+) 858 WP_205736042.1 hypothetical protein -
  C4901_RS18230 - 2879460..2879975 (+) 516 WP_205736043.1 zinc ribbon domain-containing protein -
  C4901_RS14645 (C4901_14585) - 2880108..2881184 (-) 1077 WP_110137956.1 hypothetical protein -
  C4901_RS14650 (C4901_14590) murI 2881198..2881998 (-) 801 WP_110138671.1 glutamate racemase -
  C4901_RS14655 (C4901_14595) pilU 2882121..2883233 (-) 1113 WP_110137957.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C4901_RS14660 (C4901_14600) pilT 2883235..2884305 (-) 1071 WP_110137958.1 type IV pilus twitching motility protein PilT Machinery gene
  C4901_RS14665 (C4901_14605) proC 2884409..2885230 (+) 822 WP_110137959.1 pyrroline-5-carboxylate reductase -
  C4901_RS14670 (C4901_14610) - 2885227..2885799 (+) 573 WP_110137960.1 YggT family protein -
  C4901_RS14675 (C4901_14615) - 2885796..2886281 (+) 486 WP_110137961.1 redoxin domain-containing protein -
  C4901_RS14680 (C4901_14620) - 2886287..2886466 (-) 180 WP_110137962.1 hypothetical protein -
  C4901_RS14685 (C4901_14625) trxA 2886581..2886994 (+) 414 WP_110137963.1 thioredoxin -
  C4901_RS14690 (C4901_14630) - 2887056..2888201 (+) 1146 WP_110137964.1 homoserine O-acetyltransferase -
  C4901_RS14695 (C4901_14635) metW 2888198..2888809 (+) 612 WP_110137965.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39430.05 Da        Isoelectric Point: 6.7724

>NTDB_id=278760 C4901_RS14660 WP_110137958.1 2883235..2884305(-) (pilT) [Acidiferrobacter sp. SPIII_3]
MDIAELLAFSYKQNASDLHLSAGLPPLIRVDGDVKRINVDPLDERAVHNMVYDIMNDKQQKEYEERLECDFSFELPNIAR
FRVNAFNQNRGPGAVFRTIPSKVLTLEQLNAPGIFKQIADQPRGIVLVTGPTGSGKSTTLAAMIDHVNESRREHILTIED
PIEFVHTSKNCLINQREVHRDTLGFENALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMIGTPAIRNLIRENKIAQMYSAIQTSQSVGMQTLDQCL
MELVRSRQVSPEEARGKAANKEAFTNNNGAAAAARR

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=278760 C4901_RS14660 WP_110137958.1 2883235..2884305(-) (pilT) [Acidiferrobacter sp. SPIII_3]
ATGGATATCGCGGAACTCCTGGCCTTTTCCTATAAACAGAACGCGTCGGATCTGCATTTGTCGGCCGGTCTGCCACCTCT
TATCCGCGTCGACGGCGACGTCAAGCGCATCAACGTAGACCCCTTGGATGAGCGCGCCGTGCACAATATGGTCTACGATA
TCATGAACGACAAGCAGCAAAAGGAGTATGAGGAGCGGCTGGAGTGCGATTTCTCGTTCGAGCTGCCGAACATCGCGCGT
TTTCGTGTCAATGCTTTTAATCAGAATCGTGGCCCGGGCGCCGTCTTCCGGACCATCCCCTCGAAGGTGTTGACCCTCGA
GCAATTGAACGCCCCCGGAATCTTCAAGCAGATCGCCGACCAGCCGCGCGGTATCGTACTGGTTACGGGCCCCACGGGGT
CCGGCAAATCCACCACCCTTGCGGCGATGATAGACCATGTCAACGAGAGCCGACGCGAGCACATACTGACCATAGAGGAC
CCGATCGAGTTCGTGCATACGAGCAAGAACTGCCTCATCAATCAGCGCGAGGTGCATCGGGATACCTTGGGGTTCGAGAA
TGCCCTGCGTTCGGCCTTACGTGAAGACCCGGATGTCATCCTGGTCGGCGAGTTGCGCGATCTGGAGACGATCCGCCTGG
CGCTTACCGCGGCCGAGACCGGTCATCTGGTGTTCGCCACCCTGCATACGAGTTCCGCGGCCAAGACCATAGACCGCGTG
GTGGACGTGTTCCCGGCGGCGGAGAAGGAGATGGTACGGTCCATGCTCTCGGAGTCGTTGCGCGCGGTGATCTCCCAGAC
GCTATTGAAGAAGGTCGGCGGCGGGCGCATCGCCGCCCACGAGATCATGATCGGCACGCCCGCCATCCGCAACCTGATCC
GTGAAAACAAGATCGCGCAGATGTATTCGGCCATTCAGACCAGCCAGTCCGTGGGCATGCAGACCCTCGATCAGTGCCTC
ATGGAACTCGTGCGGTCACGTCAGGTATCGCCCGAGGAGGCGCGCGGCAAGGCGGCGAACAAGGAGGCATTCACGAACAA
CAACGGGGCGGCCGCCGCCGCGCGGAGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z3R2Z3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.035

96.629

0.744
  pilT Acinetobacter baumannii D1279779

76.744

96.629

0.742
  pilT Acinetobacter baumannii strain A118

76.744

96.629

0.742
  pilT Acinetobacter nosocomialis M2

76.744

96.629

0.742
  pilT Pseudomonas stutzeri DSM 10701

76.163

96.629

0.736
  pilT Acinetobacter baylyi ADP1

73.547

96.629

0.711
  pilT Legionella pneumophila strain Lp02

70.93

96.629

0.685
  pilT Legionella pneumophila strain ERS1305867

70.93

96.629

0.685
  pilT Neisseria meningitidis 8013

68.786

97.191

0.669
  pilT Neisseria gonorrhoeae MS11

68.497

97.191

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

94.663

0.657
  pilT Vibrio cholerae strain A1552

69.436

94.663

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

96.91

0.489
  pilU Vibrio cholerae strain A1552

42.09

94.101

0.396
  pilU Acinetobacter baylyi ADP1

41.964

94.382

0.396
  pilU Pseudomonas stutzeri DSM 10701

41.194

94.101

0.388

Multiple sequence alignment