Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C4901_RS14655 Genome accession   NZ_CP027663
Coordinates   2882121..2883233 (-) Length   370 a.a.
NCBI ID   WP_110137957.1    Uniprot ID   A0A2Z3R2L2
Organism   Acidiferrobacter sp. SPIII_3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2877121..2888233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4901_RS18225 - 2878610..2879467 (+) 858 WP_205736042.1 hypothetical protein -
  C4901_RS18230 - 2879460..2879975 (+) 516 WP_205736043.1 zinc ribbon domain-containing protein -
  C4901_RS14645 (C4901_14585) - 2880108..2881184 (-) 1077 WP_110137956.1 hypothetical protein -
  C4901_RS14650 (C4901_14590) murI 2881198..2881998 (-) 801 WP_110138671.1 glutamate racemase -
  C4901_RS14655 (C4901_14595) pilU 2882121..2883233 (-) 1113 WP_110137957.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C4901_RS14660 (C4901_14600) pilT 2883235..2884305 (-) 1071 WP_110137958.1 type IV pilus twitching motility protein PilT Machinery gene
  C4901_RS14665 (C4901_14605) proC 2884409..2885230 (+) 822 WP_110137959.1 pyrroline-5-carboxylate reductase -
  C4901_RS14670 (C4901_14610) - 2885227..2885799 (+) 573 WP_110137960.1 YggT family protein -
  C4901_RS14675 (C4901_14615) - 2885796..2886281 (+) 486 WP_110137961.1 redoxin domain-containing protein -
  C4901_RS14680 (C4901_14620) - 2886287..2886466 (-) 180 WP_110137962.1 hypothetical protein -
  C4901_RS14685 (C4901_14625) trxA 2886581..2886994 (+) 414 WP_110137963.1 thioredoxin -
  C4901_RS14690 (C4901_14630) - 2887056..2888201 (+) 1146 WP_110137964.1 homoserine O-acetyltransferase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41183.26 Da        Isoelectric Point: 6.1164

>NTDB_id=278759 C4901_RS14655 WP_110137957.1 2882121..2883233(-) (pilU) [Acidiferrobacter sp. SPIII_3]
MGFLDLLRLMVEKGASDLYITAGVAPSLRVDGKLAPVTRQALTADQARQFVYSIMNEEQRSEFEETNECNFAINPQGVGR
FRVNVFRQQQRVGMVLRKIETRIPHFEELNLPPVLKDIALTKRGLVIFVGATGSGKSTSLAAMVGYRNQYSNGHIITIED
PIEFVHEHKNCIITQREVGVDTDSFETALKNTLRQAPDVILIGEVRARETMDYAIAFAETGHLCLTTLHANSANQALDRI
INFFPEDRRAQLLMDLSLNLKAVVSQRLIPKKDGAGRVAAVEIMINSPLIADLILKGEVHGIKELMAKSEEAGMQTFDQA
LFGLYEAGLIDYDEAMRNADSQNDLRLRIKLHGKDAKEAALGDSLRNITF

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=278759 C4901_RS14655 WP_110137957.1 2882121..2883233(-) (pilU) [Acidiferrobacter sp. SPIII_3]
ATGGGCTTCCTCGATCTCCTGCGACTCATGGTCGAAAAAGGCGCCTCGGACCTGTATATCACCGCCGGGGTGGCGCCCAG
CCTGCGCGTGGACGGGAAACTTGCGCCGGTGACACGCCAGGCACTCACCGCAGACCAGGCCCGCCAGTTCGTGTACAGCA
TCATGAACGAGGAGCAGCGTAGCGAGTTCGAGGAGACGAACGAGTGCAACTTCGCGATCAATCCGCAGGGTGTCGGACGC
TTTCGTGTCAACGTTTTCCGGCAGCAGCAGCGGGTTGGCATGGTGTTGCGCAAGATCGAGACCCGCATTCCGCATTTCGA
GGAATTGAATCTTCCGCCGGTATTGAAAGACATTGCCCTTACGAAGCGTGGCCTCGTGATATTCGTGGGCGCCACGGGTT
CCGGAAAGTCGACCTCGCTTGCCGCCATGGTGGGCTACCGGAATCAGTATTCGAATGGGCATATCATCACCATCGAGGAC
CCGATCGAGTTCGTGCACGAGCACAAGAACTGCATCATCACCCAGCGTGAGGTGGGCGTCGACACGGATTCGTTTGAGAC
GGCCCTGAAGAACACCCTGCGTCAGGCACCCGATGTGATCCTCATAGGCGAGGTGCGCGCGCGCGAGACGATGGACTACG
CGATCGCCTTTGCCGAGACCGGACACCTGTGCCTTACCACGCTCCATGCCAATAGCGCGAACCAGGCCCTCGATCGCATC
ATCAATTTCTTCCCGGAGGATCGGCGCGCGCAGCTCCTGATGGATCTCTCCTTGAACCTGAAGGCCGTGGTCTCGCAGCG
CCTCATTCCAAAAAAGGATGGTGCCGGACGCGTGGCGGCGGTCGAGATCATGATCAACAGTCCCTTGATCGCCGATCTCA
TCCTGAAAGGTGAGGTTCACGGCATCAAGGAGCTCATGGCCAAGTCCGAGGAGGCGGGCATGCAGACCTTCGATCAGGCG
CTGTTCGGCCTGTATGAGGCGGGCCTCATAGATTATGACGAGGCCATGCGCAACGCCGATTCGCAAAACGACCTGCGGCT
GCGCATCAAGCTGCACGGCAAGGATGCGAAAGAGGCGGCGCTCGGGGACTCCCTGCGCAACATCACCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z3R2L2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.661

94.865

0.651
  pilU Acinetobacter baylyi ADP1

63.92

95.135

0.608
  pilU Vibrio cholerae strain A1552

57.771

92.162

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.269

90.541

0.419
  pilT Pseudomonas aeruginosa PAK

45.428

91.622

0.416
  pilT Acinetobacter nosocomialis M2

45.029

92.432

0.416
  pilT Acinetobacter baumannii D1279779

45.029

92.432

0.416
  pilT Acinetobacter baumannii strain A118

45.029

92.432

0.416
  pilT Acinetobacter baylyi ADP1

44.868

92.162

0.414
  pilT Pseudomonas stutzeri DSM 10701

44.838

91.622

0.411
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.21

90.27

0.408
  pilT Vibrio cholerae strain A1552

45.21

90.27

0.408
  pilT Legionella pneumophila strain Lp02

43.582

90.541

0.395
  pilT Legionella pneumophila strain ERS1305867

43.582

90.541

0.395
  pilT Neisseria gonorrhoeae MS11

40.58

93.243

0.378
  pilT Neisseria meningitidis 8013

40.58

93.243

0.378

Multiple sequence alignment