Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SHEW_RS05865 Genome accession   NC_009092
Coordinates   1312270..1313307 (+) Length   345 a.a.
NCBI ID   WP_011864942.1    Uniprot ID   A3QC11
Organism   Shewanella loihica PV-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1307270..1318307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEW_RS05830 (Shew_1131) hemW 1307332..1308471 (-) 1140 WP_011864935.1 radical SAM family heme chaperone HemW -
  SHEW_RS05835 (Shew_1132) rdgB 1308471..1309070 (-) 600 WP_041407003.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SHEW_RS05840 (Shew_1133) - 1309212..1309646 (-) 435 WP_011864937.1 DUF4426 domain-containing protein -
  SHEW_RS05845 (Shew_1134) yggU 1309733..1310023 (-) 291 WP_011864938.1 DUF167 family protein YggU -
  SHEW_RS05850 (Shew_1135) - 1310024..1310572 (-) 549 WP_011864939.1 YggT family protein -
  SHEW_RS05855 (Shew_1136) proC 1310633..1311451 (-) 819 WP_011864940.1 pyrroline-5-carboxylate reductase -
  SHEW_RS05860 (Shew_1137) - 1311533..1312234 (-) 702 WP_011864941.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHEW_RS05865 (Shew_1138) pilT 1312270..1313307 (+) 1038 WP_011864942.1 type IV pilus twitching motility protein PilT Machinery gene
  SHEW_RS05870 (Shew_1139) pilU 1313320..1314432 (+) 1113 WP_011864943.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHEW_RS05875 (Shew_1140) hemH 1314613..1315626 (+) 1014 WP_041406501.1 ferrochelatase -
  SHEW_RS05880 (Shew_1141) - 1316043..1317056 (-) 1014 WP_011864733.1 IS110-like element ISSlo1 family transposase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38271.86 Da        Isoelectric Point: 6.9096

>NTDB_id=27709 SHEW_RS05865 WP_011864942.1 1312270..1313307(+) (pilT) [Shewanella loihica PV-4]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEKLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAGMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLITREDAMAKSSNKNASF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=27709 SHEW_RS05865 WP_011864942.1 1312270..1313307(+) (pilT) [Shewanella loihica PV-4]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAACGCGTCGGATCTACACCTTTCTGCGGGAGTCTCTCCCAT
GATACGTGTTGATGGTGAGGTCAGAAAGATTAATCTGCCGGCCCTGGATCATCAAGGCGTACACAGCCTCGTTTATGACA
TCATGAACGACAAACAGCGTAAAGATTATGAAGAGCATTTAGAGATAGATTTCTCGTTCGAAGTGCCCAACTTAGCCCGT
TTCCGTGTGAACGCCTTTAACCAGTCCCGCGGCGCCGCGGCGGTGTTCCGTACCATTCCCAGCGAGATCTTAAGCCTGGA
GAAATTGGGCGCCCCTGAGATTTTCAAGAAGATTGCCAGCTTTCCCCGTGGTCTGGTGTTGGTAACCGGCCCGACAGGTT
CGGGTAAGAGTACCACCCTGGCGGGCATGATAGATTATGTGAACGAGAACCGTCACGACCATATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCATCAGAACAAGCAGTGTCTTATCAACCAACGTGAGGTGCACAGACACACCCACAGCTTCAACGC
CGCGCTGCGTAGCGCACTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAGATGCGTGACCTCGAAACTATACGTCTGG
CGATGACGGCGGCGGAAACCGGTCACTTGGTATTTGGTACTTTGCACACCACCTCGGCGGCGAAAACCATCGACCGTGTG
GTCGACGTATTCCCAGAGGGCGAGAAGAGTATGGTGCGTACCATGTTGTCTGAGTCCCTGCAGGCGGTAATCTCCCAGAC
CCTGATCAAGAAGGTTGGCGGCGGTCGTGTGGCGGCCCACGAGATCATGATGGGTACCCCGGCGATTCGTAACCTTATTC
GCGAAGATAAGGTGGCACAGATGTACTCGGCCATTCAAACGGGTATGGCCCACGGCATGCAGACCCTAGATCAGTGTCTG
CAAAATCTGGTCAACCGTGGCCTGATCACCCGTGAAGATGCGATGGCCAAGAGTTCTAATAAAAACGCTAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3QC11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.138

96.812

0.786
  pilT Acinetobacter baumannii D1279779

81.138

96.812

0.786
  pilT Acinetobacter baumannii strain A118

81.138

96.812

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.402

97.971

0.768
  pilT Pseudomonas aeruginosa PAK

78.402

97.971

0.768
  pilT Acinetobacter baylyi ADP1

78.571

97.391

0.765
  pilT Legionella pneumophila strain Lp02

75.146

99.13

0.745
  pilT Legionella pneumophila strain ERS1305867

75.146

99.13

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

40.525

99.42

0.403
  pilU Acinetobacter baylyi ADP1

39.535

99.71

0.394

Multiple sequence alignment